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1.
Int J Med Sci ; 21(8): 1472-1490, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38903914

RESUMO

Synuclein family members (Snca, Sncb, and Scng) are expressed in the retina, but their precise locations and roles are poorly understood. We performed an extensive analysis of the single-cell transcriptome in healthy and injured retinas to investigate their expression patterns and roles. We observed the expression of all synuclein family members in retinal ganglion cells (RGCs), which remained consistent across species (human, mouse, and chicken). We unveiled differential expression of Snca across distinct clusters (highly expressed in most), while Sncb and Sncg displayed uniform expression across all clusters. Further, we observed a decreased expression in RGCs following traumatic axonal injury. However, the proportion of α-Syn-positive RGCs in all RGCs and α-Syn-positive intrinsically photosensitive retinal ganglion cells (ipRGCs) in all ipRGCs remained unaltered. Lastly, we identified changes in communication patterns preceding cell death, with particular significance in the pleiotrophin-nucleolin (Ptn-Ncl) and neural cell adhesion molecule signaling pathways, where communication differences were pronounced between cells with varying expression levels of Snca. Our study employs an innovative approach using scRNA-seq to characterize synuclein expression in health retinal cells, specifically focusing on RGC subtypes, advances our knowledge of retinal physiology and pathology.


Assuntos
Células Ganglionares da Retina , alfa-Sinucleína , gama-Sinucleína , Animais , Células Ganglionares da Retina/metabolismo , Humanos , Camundongos , alfa-Sinucleína/genética , alfa-Sinucleína/metabolismo , gama-Sinucleína/genética , gama-Sinucleína/metabolismo , beta-Sinucleína/genética , beta-Sinucleína/metabolismo , Galinhas/genética , Transcriptoma , Análise de Célula Única , Retina/metabolismo , Retina/citologia , Proteínas de Neoplasias
2.
Brain Struct Funct ; 229(5): 1279-1298, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38703218

RESUMO

ß-synuclein, a member of the synuclein family, is frequently co-expressed with α-synuclein in the neural system, where it serves to inhibit abnormal aggregation of α-synuclein in neurodegenerative diseases. Beyond its role in pathological conditions, ß-synuclein plays various functions independently of α-synuclein. In our investigation, we discovered a broader expression of ß-synuclein in the mouse retina compared to α-synuclein. This widespread pattern implies its potential significance in the retina. Through detailed examination via light- and electron-microscopic immunocytochemistry, we identified ß-synuclein expression from the inner segment (IS) and outer segment (OS) of photoreceptor cells to the ganglion cell layer (GCL). Our findings unveiled unique features, including ß-synuclein immunoreactive IS and OS of cones, higher expression in cone pedicles than in rod spherules, absence in horizontal cells, limited expression in cone bipolar dendrites and somas, higher expression in cone bipolar terminals, presence in most amacrine cells, and expression in almost majority of somas in GCL with an absence in intrinsically photosensitive retinal ganglion cell (ipRGCs) processes. Notably, all cholinergic amacrine cells express high ß- but not α-synuclein, while dopaminergic amacrine cells express α-synuclein exclusively. These distinctive expression patterns offer valuable insights for further exploration into the functions of ß-synuclein and its potential role in synuclein pathology within the retina.


Assuntos
Camundongos Endogâmicos C57BL , Retina , Células Ganglionares da Retina , alfa-Sinucleína , beta-Sinucleína , Animais , Masculino , Camundongos , alfa-Sinucleína/metabolismo , Células Amácrinas/metabolismo , beta-Sinucleína/metabolismo , Retina/metabolismo , Células Bipolares da Retina/metabolismo , Células Ganglionares da Retina/metabolismo
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