Application of TRUST, TP-ELISA and TPPA in the serologic diagnosis of syphilis / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the application value of toluidine red unheated serum test (TRUST), treponema pallidum enzyme-linked immunosorbent assay (TP-ELISA) and treponema pallidum particle agglutination assay (TPPA) in the serologic diagnosis of syphilis.</p><p><b>METHODS</b>We collected serum samples from 12 622 inpatients, 2 253 outpatients and 1 500 subjects in the medical examination center, and screened them by TRUST and TP-ELISA, followed by quantitative tests using TRUST and TPPA.</p><p><b>RESULTS</b>Among the 16 375 cases, clinically diagnosed syphilis was confirmed in 384, with a positive rate of 100% either by TRUST (1:2 -1 :16) or TPPA (1:80 - 1:2 560), and 92.2% by TP-ELISA.</p><p><b>CONCLUSION</b>TP-ELISA and TRUST should be simultaneously used for syphilis screening, but not TP-ELISA alone, and the results should be confirmed by TPPA. The combination of the three methods plays a valuable role in reducing the misdiagnosis and missed diagnosis, and contributes to the early and accurate diagnosis of syphilis.</p>

Correlation between mutation of GyrA and ParC genes and quinolone resistance in Neisseria gonorrhoeae / 中华男科学杂志

<p><b>OBJECTIVES</b>To investigate the correlation between mutation of GyrA and ParC genes and quinolone resistance in Neisseria Gonorrhoeae.</p><p><b>METHODS</b>The gene fragments of quinolone resistance-determining region (QRDR) in GyrA and ParC genes in 20 Neisseria gonorrhoeae strains clinically isolated in Wuxi, China, were sequenced, and the susceptibility of the 20 strains to quinolone was examined by agar diffusion method.</p><p><b>RESULTS</b>The mutations at the Asp95 point in GyrA gene were found in 20 strains. Of the 19 stains examined, 16 had mutations at the 86, 87, 88, 91 points in ParC genes.</p><p><b>CONCLUSIONS</b>The mutations of Asp95 in GyrA gene and Asp86, Ser87, Ser88, Glu91 in ParC gene contribute to quinolone resistance in Neisseria Gonorrhoeae.</p>