RESUMO
Mitochondrial deoxyribonucleic acid (DNA) from 201 unrelated Mongolian individuals in the three different regions was analyzed. The Mongolians took the dominant East Asian-specific haplogroups, and some European-prevalent haplogroups were detected. The East Asians-specific haplogroups distributed from east to west in decreasing frequencies, and the European-specific haplogroups distributed conversely. These genetic data suggest that the Mongolian empire played an important role in the maternal genetic admixture across Mongolians and even Central Asian populations, whereas the Silk Road might have contributed little in the admixture between the East Asians and the Europeans.
Assuntos
DNA Mitocondrial/genética , Genética Populacional , Impressão Genômica/genética , Haplótipos/genética , Polimorfismo Genético/genética , Etnicidade/genética , Variação Genética , Humanos , Mongólia , FilogeniaRESUMO
BACKGROUND: Although many arguments have been raised on the role of heredity in the etiology of acne, the relevant genetic elements in the pathogenesis of the disease are not well established. OBJECTIVE: The aim of our study was to evaluate the association between a genetic polymorphism in the promoter region of the CYP17 gene and the development of acne. METHODS: 206 acne patients and 200 controls were included in the study. A polymerase chain reaction (PCR) sequencing technique was used to reveal a CYP17 gene polymorphism in its promoter region. A chi2 test was used for data analysis. RESULTS: CYP17 -34T/C polymorphism was found and the frequency distribution of the C/C homozygotes and C allele in the male patients with severe acne (33.3 and 60.9%, respectively) were statistically significantly different from those of the control samples (18.2 and 46.6%; p < 0.05). No significant difference was observed between the female patients, mild + moderate male patients and their controls, respectively. CONCLUSION: The CYP17 -34C/C homozygote Chinese men are at a significantly increased risk of developing severe acne.
Assuntos
Acne Vulgar/genética , Polimorfismo Genético , Análise de Sequência de DNA , Esteroide 17-alfa-Hidroxilase/genética , Acne Vulgar/enzimologia , Algoritmos , Estudos de Casos e Controles , China , Feminino , Frequência do Gene , Humanos , Masculino , Razão de Chances , Reação em Cadeia da Polimerase , Análise de Sequência de DNA/métodosRESUMO
Amplification of short tandem repeat(STR) loci has become a useful tool for human identification applications. To improve throughput and efficiency for the forensic materials and gain foure and six STR locis multiplex methods with silver staining, CSF1PO,TPOX,THO1 and vWA(referred to as multiplex A), D18S51, D7S820, D13S317, D5S818, D3S1358 and Amelogenin(referred to as multiplex B) have been evaluated for use in a rape case. The products of multiplex amplication were separated in a denaturing polyacrylamide gel and analyzed with silver staining. Two multiplex amplications used in this case could provide a power of discrimination of approximately 2.43 x 10(-19). Silver staining was shown to be a validation methods for analysing the products of four and six multiplex amplications.
