How do sphingosine-1-phosphate affect immune cells to resolve inflammation?

Inflammation is an important immune response of the body. It is a physiological process of self-repair and defense against pathogens taken up by biological tissues when stimulated by damage factors such as trauma and infection. Inflammation is the main cause of high morbidity and mortality in most diseases and is the physiological basis of the disease. Targeted therapeutic strategies can achieve efficient toxicity clearance at the inflammatory site, reduce complications, and reduce mortality. Sphingosine-1-phosphate (S1P), a lipid signaling molecule, is involved in immune cell transport by binding to S1P receptors (S1PRs). It plays a key role in innate and adaptive immune responses and is closely related to inflammation. In homeostasis, lymphocytes follow an S1P concentration gradient from the tissues into circulation. One widely accepted mechanism is that during the inflammatory immune response, the S1P gradient is altered, and lymphocytes are blocked from entering the circulation and are, therefore, unable to reach the inflammatory site. However, the full mechanism of its involvement in inflammation is not fully understood. This review focuses on bacterial and viral infections, autoimmune diseases, and immunological aspects of the Sphks/S1P/S1PRs signaling pathway, highlighting their role in promoting intradial-adaptive immune interactions. How S1P signaling is regulated in inflammation and how S1P shapes immune responses through immune cells are explained in detail. We teased apart the immune cell composition of S1P signaling and the critical role of S1P pathway modulators in the host inflammatory immune system. By understanding the role of S1P in the pathogenesis of inflammatory diseases, we linked the genomic studies of S1P-targeted drugs in inflammatory diseases to provide a basis for targeted drug development.

Biodiversity and degradation potential of oil-degrading bacteria isolated from sediments of hydrothermal and non-hydrothermal areas of the Southwest Mid-Indian Ocean Ridge.

In this study, sediments from eight sites were collected from hydrothermal areas (e.g., the Tiancheng, Tianzuo, and Longqi hydrothermal areas) and non-hydrothermal area on the Southwest Mid-Indian Ocean Ridge. Using crude oil as the only carbon and energy source, 162 strains of culturable oil-degrading bacteria were isolated and obtained. The rate of oil degradation of the consortia was 39.48-46.00% in hydrothermal and non-hydrothermal areas. High-throughput sequencing found that the alpha diversity indices (e.g., Shannon and Simpson) of the communities in hydrothermal areas were higher than those in non-hydrothermal area. The species diversities of the oil-degrading bacteria were different among different hydrothermal areas. The composition of the oil-degrading bacterial species in the Tianzuo hydrothermal area tended to be more similar to that in the non-hydrothermal area. This similarity is attributed to the changes in the bacterial community that followed the cessation of hydrothermal vent eruptions at this site. The Alphaproteobacteria abundance of the oil-degrading bacteria was significantly different in oil-degrading bacteria between the hydrothermal and non-hydrothermal areas.

New IT driven rapid manufacturing for emergency response.

COVID-19, which is rampant around the world, has seriously disrupted people's normal work and living. To respond to public urgent needs such as COVID-19, emergency supplies are essential. However, due to the special requirements of supplies, when an emergency occurs, the supply reserve mostly cannot cope with the high demand. Given the importance of emergency supplies in public emergencies, rapid response manufacturing of emergency supplies is a necessity. The faster emergency supplies and facilities are manufactured, the more likely the pandemic can be controlled and the more human lives are saved. Besides, new generation information technology represented by cloud computing, IoT, big data, AI, etc. is rapidly developing and can be widely used to address such situations. Therefore, rapid response manufacturing enabled by New IT is presented to quickly meet emergency demands. And some policy suggestions are presented.

An endophytic bacterial strain, Enterobacter cloacae TMX-6, enhances the degradation of thiamethoxam in rice plants.

Thiamethoxam (TMX) has been widely used over the last two decades. TMX residue in the environment has drawn great public attention. An endophytic bacterial strain, TMX-6, capable of degrading TMX was isolated from wild Ophiopogon japonicus and was identified as Enterobacter cloacae by morphology and 16S ribosomal DNA sequence analysis. After being marked with green fluorescent protein plasmid, TMX-6 was successfully inoculated in the rice plants (Oryza sativa L.). The numbers of TMX-6 in non-TMX treated rice plants ranged from 3.9 to 4.6 log CFU g-1 in the roots, and from 2.7 to 4.0 log CFU g-1 in the shoots; while ranged from 3.9 to 5.3 log CFU g-1 in roots and from 2.7 to 4.1 log CFU g-1 in shoots of TMX treated rice plants. Nearly 28%, 33%, 77% and 99% of TMX was removed from the hydroponic medium (HM), HM with strain TMX-6, HM with uninoculated rice and HM with inoculated rice, respectively, at the end of a 21-day (d) experiment period, and the correspondent half-lives of TMX were 46.2, 38.5, 9.9 and 4.7 d, respectively. Eleven TMX metabolites were identified in both inoculated and uninoculated rice plants through metabolomics data analysis. The intensity of TMX- NH, TMX-urea and clothianidin increased more than 3 times in inoculated rice plants on day 6. This demonstrates the usefulness of the strain TMX-6 to enhance the degradation of TMX-contaminated substrates and reduce levels of toxic insecticides in crop plants.

Uptake and translocation of imidacloprid, thiamethoxam and difenoconazole in rice plants.

Uptake and translocation of imidacloprid (IMI), thiamethoxam (THX) and difenoconazole (DFZ) in rice plants (Oryza sativa L.) were investigated with a soil-treated experiment at two application rates: field rate (FR) and 10*FR under laboratory conditions. The dissipation of the three compounds in soil followed the first-order kinetics and DFZ showed greater half-lives than IMI and THX. Detection of the three compounds in rice tissues indicated that rice plants could take up and accumulate these pesticides. The concentrations of IMI and THX detected in leaves (IMI, 10.0 and 410 mg/kg dw; THX, 23.0 and 265 mg/kg dw) were much greater than those in roots (IMI, 1.37 and 69.3 mg/kg dw; THX, 3.19 and 30.6 mg/kg dw), which differed from DFZ. The DFZ concentrations in roots (15.6 and 79.1 mg/kg dw) were much greater than those in leaves (0.23 and 3.4 mg/kg dw). The bioconcentration factor (BCF), representing the capability of rice to accumulate contaminants from soil into plant tissues, ranged from 1.9 to 224.3 for IMI, from 2.0 to 72.3 for THX, and from 0.4 to 3.2 for DFZ at different treated concentrations. Much higher BCFs were found for IMI and THX at 10*FR treatment than those at FR treatment, however, the BCFs of DFZ at both treatments were similar. The translocation factors (TFs), evaluating the capability of rice to translocate contaminants from the roots to the aboveground parts, ranged from 0.02 to 0.2 for stems and from 0.02 to 9.0 for leaves. The tested compounds were poorly translocated from roots to stems, with a TF below 1. However, IMI and THX were well translocated from roots to leaves. Clothianidin (CLO), the main metabolite of THX, was detected at the concentrations from 0.02 to 0.5 mg kg-1 in soil and from 0.07 to 7.0 mg kg-1 in plants. Concentrations of CLO in leaves were almost 14 times greater than those in roots at 10*FR treatment.

Understanding molecular mechanism of higher plant plasticity under abiotic stress.

Higher plants play the most important role in keeping a stable environment on the earth, which regulate global circumstances in many ways in terms of different levels (molecular, individual, community, and so on), but the nature of the mechanism is gene expression and control temporally and spatially at the molecular level. In persistently changing environment, there are many adverse stress conditions such as cold, drought, salinity and UV-B (280-320 mm), which influence plant growth and crop production greatly. Plants differ from animals in many aspects, but the important may be that plants are more easily influenced by environment than animals. Plants have a series of fine mechanisms for responding to environmental changes, which has been established during their long-period evolution and artificial domestication. These mechanisms are involved in many aspects of anatomy, physiology, biochemistry, genetics, development, evolution and molecular biology, in which the adaptive machinery related to molecular biology is the most important. The elucidation of it will extremely and purposefully promote the sustainable utilization of plant resources and make the best use of its current potential under different scales. This molecular mechanism at least include environmental signal recognition (input), signal transduction (many cascade biochemical reactions are involved in this process), signal output, signal responses and phenotype realization, which is a multi-dimensional network system and contain many levels of gene expression and regulation. We will focus on the molecular adaptive machinery of higher plant plasticity under abiotic stresses.