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1.
Intensive Care Med Exp ; 4(1): 24, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27456956

RESUMO

BACKGROUND: The development of lactic acidemia (LA) in septic shock (SS) is associated with an ominous prognosis. We previously showed that the mechanism of LA in SS may relate to impaired hepatic uptake of lactate, but the mechanism was not clear. Uptake of lactate by the liver occurs by a membrane-associated, pH-dependent, antiport system known as the monocarboxylate transporter. In the hepatocyte, lactate can then be metabolized by oxidative phosphorylation or converted to glucose in the cytosol. In the present study, we examined (1) whether hepatic mitochondrial dysfunction accounted for decreased uptake of lactate in a canine model of Pseudomonas aeruginosa SS, (2) whether norepinephrine (NE) treatment by increasing mean arterial pressure (MAP) could improve mitochondrial dysfunction and LA in this model, and (3) whether gentisic acid sodium salt (GSS), a novel phenolic compound, was superior to NE in these effects. METHODS: In anesthetized/ventilated dogs, we infused the bacteria over ~10 h and measured hemodynamics in various treatment groups (see below). We then euthanized the animal and isolated the hepatic mitochondria. We measured hepatic mitochondrial oxygen consumption rates using the novel Seahorse XF24 analyzer under conditions that included: basal respiration, after the addition of adenosine- diphosphate to produce coupled respiration, and after the addition of a protonophore to produce maximal respiration. RESULTS: We found that in the septic control group, mean arterial pressure decreased over the course of the study, and that mitochondrial dysfunction developed in which there was a reduction in maximal respiration. Whereas both NE and GSS treatments reversed the reduction in mean arterial pressure and increased maximal respiration to similar extents in respective groups, only in the GSS group was there a reduction in LA. CONCLUSIONS: Hepatic mitochondrial dysfunction occurs in SS, but does not appear to be required for the development of LA in SS, since NE improved mitochondrial dysfunction without reversing LA. GSS, a phenolic compound restored mean arterial pressure, mitochondrial dysfunction, and LA in SS. This reduction in LA may be independent of its effect on improving hepatic mitochondrial function.

2.
Shock ; 41(3): 256-65, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24296430

RESUMO

In septic shock (SS), dysfunction of many organ systems develops during the course of the illness, although the mechanisms are not clear. In earlier studies, we reported that lysozyme-c (Lzm-S), a protein that is released from leukocytes and macrophages, was a mediator of the myocardial depression and vasodilation that develop in a canine model of Pseudomonas aeruginosa SS. Whereas both of these effects of Lzm-S are dependent on its ability to intrinsically generate hydrogen peroxide, we subsequently showed that Lzm-S can also deposit within the vascular smooth muscle layer of the systemic arteries in this model. In the present study, we extend our previous findings. We used a canine carotid artery organ bath preparation to study the time course and dose dependence of Lzm-S deposition within the vascular smooth muscle layer. We used a human aortic vascular smooth muscle cell preparation to determine whether Lzm-S can persistently inhibit contraction in this preparation. We also used a canine P. aeruginosa model to determine whether Lzm-S deposition might occur in other organs such as the kidney, liver, and small intestine. The results showed that, in the carotid artery organ bath preparation, Lzm-S deposition occurred within minutes of instillation and there was a dose-response effect. In the human aortic vascular smooth muscle cell preparation, Lzm-S inhibited contraction during a 4-day period. In the in vivo model, Lzm-S accumulated in the kidney and the superior mesenteric artery. In a canine renal epithelial preparation, we further showed that Lzm-S can be taken up by the renal tubules to activate inflammatory pathways. We conclude that Lzm-S can deposit in the systemic vasculature and kidneys in SS, where this deposition could lead to acute organ dysfunction.


Assuntos
Túbulos Renais Distais/enzimologia , Macrófagos/enzimologia , Muramidase/metabolismo , Músculo Liso Vascular/enzimologia , Miócitos de Músculo Liso/enzimologia , Sepse/enzimologia , Animais , Aorta/enzimologia , Aorta/patologia , Artérias Carótidas/enzimologia , Artérias Carótidas/patologia , Células Cultivadas , Modelos Animais de Doenças , Cães , Humanos , Intestino Delgado/enzimologia , Intestino Delgado/patologia , Túbulos Renais Distais/patologia , Artérias Mesentéricas/enzimologia , Artérias Mesentéricas/patologia , Músculo Liso Vascular/patologia , Miócitos de Músculo Liso/patologia , Técnicas de Cultura de Órgãos , Infecções por Pseudomonas/enzimologia , Infecções por Pseudomonas/patologia , Pseudomonas aeruginosa , Sepse/patologia
3.
J Appl Physiol (1985) ; 112(4): 638-50, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22096116

RESUMO

In septic shock (SS), cardiovascular collapse is caused by the release of inflammatory mediators. We previously found that lysozyme-c (Lzm-S), released from leukocytes, contributed to systemic vasodilation in a canine model of SS. We then delineated the pathway by which this occurs in a canine carotid artery organ bath preparation (CAP). We showed that Lzm-S could intrinsically generate hydrogen peroxide (H(2)O(2)) and that H(2)O(2) subsequently reacted with endogenous catalase to form compound I, an oxidized form of catalase. In turn, compound I led to an increase in cyclic guanosine 3',5'-monophosphate to produce vasodilation. However, it was not clear from previous studies whether it is necessary for Lzm-S to bind to the vasculature to cause vasodilation or, alternatively, whether the generation of H(2)O(2) by Lzm-S in the surrounding medium is all that is required. We examined this question in the present study in which we used multiple preparations. In a partitioned CAP, we found that when we added Lzm-S to a partitioned space in which a semipermeable membrane prevented diffusion of Lzm-S to the carotid artery tissue, vasodilation still occurred because of diffusion of H(2)O(2). On the other hand, we found that Lzm-S could accumulate within the vascular smooth muscle layer (VSML) after 7 h of SS in a canine model. We also determined that when Lzm-S was located in close proximity to vascular smooth muscle cells, it could generate H(2)O(2) to produce lengthening in a human cell culture preparation. We conclude that there are two mechanisms by which Lzm-S can cause vasodilation in SS. In one instance, H(2)O(2) generated by Lzm-S in plasma diffuses to the VSML to cause vasodilation. In a second mechanism, Lzm-S directly binds to the VSML, where it generates H(2)O(2) to produce vasodilation.


Assuntos
Muramidase/fisiologia , Miócitos de Músculo Liso/fisiologia , Choque Séptico/fisiopatologia , Vasodilatação , Animais , Células Cultivadas , Cães , Ácido Gálico/análogos & derivados , Humanos , Técnicas In Vitro
4.
Crit Care Med ; 40(2): 560-72, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22020237

RESUMO

INTERVENTIONS: Vasopressor therapy is required in septic shock to maintain tissue perfusion in the face of hypotension. Unfortunately, there are significant side effects of current vasopressors, and newer agents need to be developed. We recently discovered that ethyl gallate, a nonflavonoid phenolic antioxidant found in food substances, could reverse low mean arterial pressure found in an experimental model of septic shock due to inhibition of hydrogen peroxide signaling. In the present study, we compared the hemodynamic and biochemical effects of ethyl gallate vs. those of the commonly used vasopressor, norepinephrine, in a bacteremic canine model of Pseudomonas aeruginosa sepsis in two protocols. MEASUREMENTS AND MAIN RESULTS: We performed these studies in anesthetized and mechanically ventilated dogs. In the early treatment protocol, we infused P. aeruginosa until mean arterial pressure first decreased to ∼60 mm Hg (about 2-3 hrs), after which we stopped the infusion and randomly administered ethyl gallate or norepinephrine in respective groups. In the late treatment protocol, we administered ethyl gallate or norepinephrine after a sustained ∼5-hr decrease in mean arterial pressure to 60 mm Hg and continued the infusion for the duration of the experiment. We followed parameters for over 10 hrs after the initiation of P. aeruginosa in both groups. We measured stroke work, urine output, serum creatinine, among other parameters, and used serum troponin T as an index of myocardial injury. We found that in both protocols, ethyl gallate and norepinephrine improved mean arterial pressure and stroke work to similar extents over the duration of the study. Particularly in the late treatment protocol, ethyl gallate resulted in a lower heart rate, a lower troponin T, and a greater urine output as compared with norepinephrine (p < .05). CONCLUSIONS: These results suggest that phenolic antioxidants, such as ethyl gallate, that inhibit hydrogen peroxide signaling, may represent an alternative class of vasopressors for use in septic shock.


Assuntos
Ácido Gálico/análogos & derivados , Norepinefrina/farmacologia , Infecções por Pseudomonas/tratamento farmacológico , Pseudomonas aeruginosa , Choque Cardiogênico/tratamento farmacológico , Choque Séptico/tratamento farmacológico , Análise de Variância , Animais , Modelos Animais de Doenças , Cães , Relação Dose-Resposta a Droga , Esquema de Medicação , Ácido Gálico/farmacologia , Infecções por Pseudomonas/mortalidade , Distribuição Aleatória , Medição de Risco , Choque Cardiogênico/diagnóstico , Choque Cardiogênico/mortalidade , Choque Séptico/microbiologia , Choque Séptico/mortalidade , Taxa de Sobrevida , Resultado do Tratamento
5.
J Appl Physiol (1985) ; 110(2): 359-74, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21071593

RESUMO

Although hydrogen peroxide (H2O2) is a well-described reactive oxygen species that is known for its cytotoxic effects and associated tissue injury, H2O2 has recently been established as an important signaling molecule. We previously demonstrated that lysozyme (Lzm-S), a mediator of sepsis that is released from leukocytes, could produce vasodilation in a phenylephrine-constricted carotid artery preparation by H2O2 signaling. We found that Lzm-S could intrinsically generate H2O2 and that this generation activated H2O2-dependent pathways. In the present study, we used this carotid artery preparation as a bioassay to define those antioxidants that could inhibit Lzm-S's vasodilatory effect. We then determined whether this antioxidant could reverse the hypotension that developed in an Escherichia coli bacteremic model. Of the many antioxidants tested, we found that ethyl gallate (EG), a nonflavonoid phenolic compound, was favorable in inhibiting Lzm-S-induced vasodilation. In our E. coli model, we found that EG reversed the hypotension that developed in this model and attenuated end-organ dysfunction. By fluorometric H2O2 assay and electrochemical probe techniques, we showed that EG could scavenge H2O2 and that it could reduce H2O2 production in model systems. These results show that EG, an antioxidant that was found to scavenge H2O2 in vitro, was able to attenuate cardiovascular dysfunction in a canine in vivo preparation. Antioxidants such as EG may be useful in the treatment of hemodynamic deterioration in septic shock.


Assuntos
Ácido Gálico/análogos & derivados , Peróxido de Hidrogênio/metabolismo , Hipotensão/prevenção & controle , Hipotensão/fisiopatologia , Muramidase/antagonistas & inibidores , Muramidase/farmacologia , Choque Séptico/fisiopatologia , Animais , Cães , Sequestradores de Radicais Livres/administração & dosagem , Ácido Gálico/administração & dosagem , Hipotensão/etiologia , Técnicas In Vitro , Choque Séptico/complicações , Choque Séptico/tratamento farmacológico , Transdução de Sinais/efeitos dos fármacos , Resultado do Tratamento , Vasodilatação/efeitos dos fármacos
6.
Am J Physiol Heart Circ Physiol ; 297(3): H930-48, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19542485

RESUMO

In septic shock, cardiovascular collapse is caused by the release of inflammatory mediators. We previously found that lysozyme (Lzm-S), released from leukocytes, contributed to the myocardial depression and arterial vasodilation that develop in canine models of septic shock. To cause vasodilation, Lzm-S generates hydrogen peroxide (H(2)O(2)) that activates the smooth muscle soluble guanylate cyclase (sGC) pathway, although the mechanism of H(2)O(2) generation is not known. To cause myocardial depression, Lzm-S binds to the endocardial endothelium, resulting in the formation of nitric oxide (NO) and subsequent activation of myocardial sGC, although the initial signaling event is not clear. In this study, we examined whether the myocardial depression produced by Lzm-S was also caused by the generation of H(2)O(2) and whether Lzm-S could intrinsically generate H(2)O(2) as has been described for other protein types. In a canine ventricular trabecular preparation, we found that the peroxidizing agent Aspergillus niger catalase, that would breakdown H(2)O(2), prevented Lzm-S- induced decrease in contraction. We also found that compound I, a species of catalase formed during H(2)O(2) metabolism, could contribute to the NO generation caused by Lzm-S. In tissue-free experiments, we used a fluorometric assay (Ultra Amplex red H(2)O(2) assay) and electrochemical sensor techniques, respectively, to measure H(2)O(2) generation. We found that Lzm-S could generate H(2)O(2) and, furthermore, that this generation could be attenuated by the singlet oxygen quencher sodium azide. This study shows that Lzm-S, a mediator of sepsis, is able to intrinsically generate H(2)O(2). Moreover, this generation may activate H(2)O(2)-dependent pathways leading to cardiovascular collapse in septic shock.


Assuntos
Doenças Cardiovasculares/metabolismo , Peróxido de Hidrogênio/metabolismo , Muramidase/metabolismo , Contração Miocárdica/fisiologia , Choque Séptico/metabolismo , Animais , Aspergillus niger/enzimologia , Doenças Cardiovasculares/imunologia , Catalase/farmacologia , Cães , Inibidores Enzimáticos/farmacologia , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase Tipo III/antagonistas & inibidores , Óxido Nítrico Sintase Tipo III/metabolismo , Consumo de Oxigênio/fisiologia , Espécies Reativas de Oxigênio/metabolismo , Choque Séptico/imunologia , ômega-N-Metilarginina/farmacologia
7.
Am J Physiol Heart Circ Physiol ; 294(4): H1724-35, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18263714

RESUMO

In septic shock, systemic vasodilation and myocardial depression contribute to the systemic hypotension observed. Both components can be attributed to the effects of mediators that are released as part of the inflammatory response. We previously found that lysozyme (Lzm-S), released from leukocytes, contributed to the myocardial depression that develops in a canine model of septic shock. Lzm-S binds to the endocardial endothelium, resulting in the production of nitric oxide (NO), which, in turn, activates the myocardial soluble guanylate cyclase (sGC) pathway. In the present study, we determined whether Lzm-S might also play a role in the systemic vasodilation that occurs in septic shock. In a phenylephrine-contracted canine carotid artery ring preparation, we found that both canine and human Lzm-S, at concentrations similar to those found in sepsis, produced vasorelaxation. This decrease in force could not be prevented by inhibitors of NO synthase, prostaglandin synthesis, or potassium channel inhibitors and was not dependent on the presence of the vascular endothelium. However, inhibitors of the sGC pathway prevented the vasodilatory activity of Lzm-S. In addition, Aspergillus niger catalase, which breaks down H(2)O(2), as well as hydroxyl radical scavengers, which included hydroquinone and mannitol, prevented the effect of Lzm-S. Electrochemical sensors corroborated that Lzm-S caused H(2)O(2) release from the carotid artery preparation. In conclusion, these results support the notion that when Lzm-S interacts with the arterial vasculature, this interaction results in the formation of H(2)O(2), which, in turn, activates the sGC pathway to cause relaxation. Lzm-S may contribute to the vasodilation that occurs in septic shock.


Assuntos
Artéria Carótida Interna/metabolismo , Peróxido de Hidrogênio/metabolismo , Artéria Mesentérica Superior/metabolismo , Muramidase/metabolismo , Óxido Nítrico/metabolismo , Sepse/metabolismo , Transdução de Sinais , Vasodilatação , Aminoquinolinas/farmacologia , Animais , Artéria Carótida Interna/efeitos dos fármacos , Artéria Carótida Interna/enzimologia , Catalase/metabolismo , GMP Cíclico/análogos & derivados , GMP Cíclico/metabolismo , GMP Cíclico/farmacologia , Proteínas Quinases Dependentes de GMP Cíclico/antagonistas & inibidores , Proteínas Quinases Dependentes de GMP Cíclico/metabolismo , Inibidores de Ciclo-Oxigenase/farmacologia , Cães , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/farmacologia , Etanol/farmacologia , Sequestradores de Radicais Livres/farmacologia , Guanilato Ciclase/antagonistas & inibidores , Guanilato Ciclase/metabolismo , Humanos , Hidroquinonas/farmacologia , Técnicas In Vitro , Indometacina/farmacologia , Manitol/farmacologia , Artéria Mesentérica Superior/efeitos dos fármacos , Artéria Mesentérica Superior/enzimologia , Azul de Metileno/farmacologia , Muramidase/antagonistas & inibidores , Óxido Nítrico Sintase/antagonistas & inibidores , Óxido Nítrico Sintase/metabolismo , Oxidiazóis/farmacologia , Fenilefrina/farmacologia , Prostaglandinas/metabolismo , Inibidores de Proteínas Quinases/farmacologia , Quinoxalinas/farmacologia , Receptores Citoplasmáticos e Nucleares/antagonistas & inibidores , Receptores Citoplasmáticos e Nucleares/metabolismo , Sepse/fisiopatologia , Transdução de Sinais/efeitos dos fármacos , Guanilil Ciclase Solúvel , Tionucleotídeos/farmacologia , Fatores de Tempo , Vasoconstritores/farmacologia , Vasodilatação/efeitos dos fármacos , ômega-N-Metilarginina/farmacologia
8.
Shock ; 29(6): 681-7, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-17885642

RESUMO

Cardiovascular dysfunction in septic shock (SS) is ascribed to the release of inflammatory mediators. Norepinephrine (NE) is often administered to treat low MAP in SS. We recently found that lysozyme c (Lzm-S) released from leukocytes was a mediator of myocardial depression in an Escherichia coil model of SS in dogs. This effect can be blocked in an in vitro preparation by chitobiose, a competitive inhibitor of Lzm-S. In the present study, we examined whether chitobiose treatment can reverse myocardial depression and obviate NE requirements in two respective canine E. coli preparations. In a 6-h study, we administered chitobiose after 3.5 h of E. coli bacteremia and compared stroke work (SW) and MAP at 6 h with a sepsis control group. In a 12-h study, we determined whether chitobiose treatment can reduce the need for NE requirements during 12 h of bacteremia. In the latter study, either chitobiose or NE was given when MAP decreased approximately 20% from the presepsis value in respective groups. In anesthetized, mechanically ventilated dogs, we monitored hemodynamic parameters during continuous E. coli infusion. In the 6-h study, chitobiose improved SW and MAP at the 6-h period as compared with the nontreated sepsis group. In the 12-h study, SW and MAP increased after chitobiose without the necessity of NE administration. These results suggest that inhibitors of Lzm-S such as chitobiose may improve myocardial depression and reduce the need for NE requirements in SS.


Assuntos
Cardiomiopatias/tratamento farmacológico , Dissacarídeos/farmacologia , Inibidores Enzimáticos/farmacologia , Infecções por Escherichia coli/tratamento farmacológico , Escherichia coli , Muramidase/antagonistas & inibidores , Norepinefrina/farmacologia , Choque Séptico/tratamento farmacológico , Vasoconstritores/farmacologia , Animais , Bacteriemia/tratamento farmacológico , Bacteriemia/enzimologia , Bacteriemia/fisiopatologia , Cardiomiopatias/enzimologia , Cardiomiopatias/fisiopatologia , Cães , Infecções por Escherichia coli/enzimologia , Infecções por Escherichia coli/fisiopatologia , Humanos , Mediadores da Inflamação/metabolismo , Masculino , Choque Séptico/enzimologia , Choque Séptico/fisiopatologia , Volume Sistólico/efeitos dos fármacos , Fatores de Tempo
9.
Am J Physiol Heart Circ Physiol ; 293(5): H3140-9, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17766478

RESUMO

We previously showed that lysozyme (Lzm-S), derived from leukocytes, caused myocardial depression in canine sepsis by binding to the endocardial endothelium to release nitric oxide (NO). NO then diffuses to adjacent myocytes to activate the cGMP pathway. In a canine right ventricular trabecular (RVT) preparation, Lzm-S also decreased the inotropic response to field stimulation (FSR) during which the sympathetic and parasympathetic nerves were simulated to measure the adrenergic response. In the present study, we determined whether the pathway by which Lzm-S decreased FSR was different from the pathway by which Lzm-S reduced steady-state (SS) contraction. Furthermore, we determined whether the decrease in FSR was due to a decrease in sympathetic stimulation or enhanced parasympathetic signaling. In the RVT preparation, we found that the inhibitory effect of Lzm-S on FSR was prevented by NO synthase (NOS) inhibitors. A cGMP inhibitor also blocked the depressant activity of Lzm-S. However, in contrast to the Lzm-S-induced decline in SS contraction, chemical removal of the endocardial endothelium by Triton X-100 to eliminate endothelial NO release did not prevent the decrease in FSR. An inhibitory G protein was involved in the effect of Lzm-S, since FSR could be restored by treatment with pertussis toxin. Atropine prevented the Lzm-S-induced decline in FSR, whereas beta(1)- and beta(2)-adrenoceptor function was not impaired by Lzm-S. These results indicate that the Lzm-S-induced decrease in FSR results from a nonendothelial release of NO. NO then acts through inhibitory G protein to enhance parasympathetic signaling.


Assuntos
Proteínas de Ligação ao GTP/metabolismo , Muramidase/metabolismo , Miocardite/metabolismo , Óxido Nítrico/metabolismo , Receptores Adrenérgicos beta/metabolismo , Sepse/metabolismo , Transdução de Sinais , Sistema Nervoso Simpático/fisiopatologia , Animais , Cães , Estimulação Elétrica , Endotélio Vascular/metabolismo
10.
J Mol Cell Cardiol ; 39(4): 615-25, 2005 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-16087190

RESUMO

Inflammatory mediators have been implicated as a cause of reversible myocardial depression in septic shock. We previously reported that the release of lysozyme-c (Lmz-S) from leukocytes from the spleen or other organs contributes to myocardial dysfunction in Escherichia coli septic shock in dogs by binding to a cardiac membrane glycoprotein. However, the mechanism by which Lzm-S causes this depression has not been elucidated. In the present study, we tested the hypothesis that the binding of Lzm-S to a membrane glycoprotein causes myocardial depression by the formation of nitric oxide (NO). NO generation then activates soluble guanylyl cyclase and increases cyclic guanosine monophosphate (cGMP), which in turn triggers contractile impairment via activation of cGMP-dependent protein kinase (PKG). We examined these possibilities in a right ventricular trabecular preparation in which isometric contraction was used to measure cardiac contractility. We found that Lzm-S's depressant effect could be prevented by the non-specific NO synthase (NOS) inhibitor N(G)-monomethyl-l-arginine (l-NMMA). A guanylyl cyclase inhibitor (ODQ) and a PKG inhibitor (Rp-8-Br-cGMP) also attenuated Lzm-S's depressant effect as did chemical denudation of the endocardial endothelium (EE) with Triton X-100 (0.5%). In EE tissue, we further showed that Lzm-S caused NO release with use of 4,5 diaminofluorescein, a fluorescent dye that binds to NO. The present study shows that the binding of Lzm-S to EE generates NO, and that NO then activates the myocardial guanosine 3',5' monophosphate pathway leading to cardiac depression in sepsis.


Assuntos
GMP Cíclico/metabolismo , Endocárdio/metabolismo , Muramidase/farmacologia , Contração Miocárdica , Óxido Nítrico/metabolismo , Sepse/metabolismo , Sepse/fisiopatologia , Animais , Membrana Celular/metabolismo , GMP Cíclico/análogos & derivados , GMP Cíclico/farmacologia , Cães , Endocárdio/citologia , Endocárdio/efeitos dos fármacos , Endotélio/efeitos dos fármacos , Endotélio/metabolismo , Técnicas In Vitro , Muramidase/metabolismo , Contração Miocárdica/efeitos dos fármacos , Miocárdio/metabolismo , Oxidiazóis/farmacologia , Quinoxalinas/farmacologia , ômega-N-Metilarginina/farmacologia
11.
Intensive Care Med ; 31(1): 129-37, 2005 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-15605233

RESUMO

PURPOSE: In sepsis, reversible myocardial depression has been ascribed to the release of mediators of inflammation. We previously found that lysozyme released from leukocytes from the spleen and other organs mediated myocardial depression in an Escherichia coli model of septic shock in dogs. We hypothesize that lysozyme binds to or cleaves a cardiac surface membrane N-glycoprotein to cause depression. The objectives of the present study were: 1) to determine whether the binding of lysozyme is reversible; 2) to assess the N-glycan structure to which lysozyme binds; 3) to examine whether nonenzymatic proteins, termed lectins, with a binding specificity similar to that of lysozyme could also cause depression; and 4) to assess whether the membrane to which lysozyme binds is affected by the enzymes protease type XIV and collagenase A, that are used to prepare single cell myocyte experiments. METHODS: We measured isometric contraction in a right ventricular trabecular preparation. RESULTS: We found that lysozyme binds in a reversible manner to the Man beta(1-4) GlcNAc beta(1-4)GlcNAc moiety in the tri-mannosyl core structure of high mannose/hybrid and tri-antennary carbohydrate classes where GlcNAc is N-acetylglucosamine and Man is mannose. Lectins with a specificity similar to that of lysozyme also caused depression, and lysozyme's depressant activity was eliminated by protease type XIV and collagenase A. CONCLUSIONS: These results indicate that lysozyme reversibly binds to a membrane glycoprotein to cause myocardial depression in sepsis. We further localize its binding site to a variant of the chitotriose structure in the tri-mannosyl core of the membrane glycoprotein.


Assuntos
Glicoproteínas de Membrana/metabolismo , Muramidase/metabolismo , Oligossacarídeos/metabolismo , Choque Séptico/metabolismo , Animais , Sítios de Ligação , Cardiomiopatias/etiologia , Cães , Lectinas/farmacologia , Contração Miocárdica/efeitos dos fármacos
12.
Crit Care Med ; 32(1): 184-93, 2004 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-14707578

RESUMO

OBJECTIVE: Reversible myocardial depression in sepsis has been ascribed to the release of inflammatory mediators. We recently found that lysozyme c (Lzm-S), consistent with that originating from the spleen, was a mediator of myocardial depression in an Escherichia coli model of septic shock in dogs. We further showed in a right ventricular trabecular (RVT) preparation that Lzm-S's depressant activity could be blocked by N,N',N" triacetylglucosamine (TAC), a competitive inhibitor of Lzm-S. We hypothesized that Lzm-S binds to or cleaves a cardiac membrane glycoprotein, thereby interfering with myocardial contraction in sepsis. In the present study, we examined whether TAC could prevent myocardial depression in an in vivo preparation and whether other related N-acetylglucosamine (NAG) structures could also inhibit Lzm-S's effect in RVT. DESIGN: Randomized experimental study. SETTING: University laboratory. SUBJECTS: Anesthetized, mechanically ventilated dogs. INTERVENTIONS: We produced sepsis by infusion of E. coli over an approximately 6-hr period. MEASUREMENTS AND MAIN RESULTS: We examined the effect of TAC on stroke work, our primary index of myocardial function, when treatment was administered before sepsis (pretreatment) and after 1.5 hrs (early treatment study) and 3.5 hrs of sepsis (late treatment study; LTS). In the pretreatment study and early treatment study, myocardial depression would have not yet occurred but would have already been present in the late treatment study. In RVT, we assessed the effect of other NAG oligosaccharides and variants to the NAG structure on Lzm-S's depressant activity. In pretreatment and the early treatment study, TAC prevented the reduction in stroke work observed in nontreated septic groups but did not reverse the reduction found in the late treatment study. In RVT, of the compounds tested, only N,N'-diacetylglucosamine showed an inhibitory effect. CONCLUSIONS: We found that TAC, a competitive inhibitor of Lzm-S, prevented myocardial depression in experimental sepsis. Only specific NAG structures are inhibitory to Lzm-S's depressant activity. TAC may be useful in attenuating cardiovascular collapse in sepsis.


Assuntos
Acetilglucosaminidase/farmacologia , Débito Cardíaco/efeitos dos fármacos , Infecções por Escherichia coli/tratamento farmacológico , Muramidase/metabolismo , Choque Séptico/tratamento farmacológico , Disfunção Ventricular Esquerda/prevenção & controle , Animais , Modelos Animais de Doenças , Cães , Feminino , Masculino , Muramidase/efeitos dos fármacos , Contração Miocárdica/efeitos dos fármacos , Fator Depressor Miocárdico/análise , Probabilidade , Distribuição Aleatória , Valores de Referência , Sensibilidade e Especificidade , Volume Sistólico/efeitos dos fármacos
13.
J Mol Cell Cardiol ; 35(3): 265-75, 2003 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-12676541

RESUMO

The objective of the present study was to identify the nature of a filterable cardiodepressant substance (FCS) that contributes to myocardial dysfunction in a canine model of Escherichia coli septic shock. In a previous study, it was found that FCS increased in plasma after 4 h of bacteremia (Am J Physiol 1993;264:H1402) in which FCS was identified by a bioassay that included a right ventricular trabecular (RVT) preparation. In that study, FCS was only partially identified by pore filtration techniques and was found to be a protein of molecular weight between 10 and 30 K. In the present study, FCS was further purified by size exclusion high-pressure liquid chromatography, until a single band was identified on one-dimensional gel electrophoresis. This band was then subjected to tandem mass spectrometry and protein-sequencing techniques and both techniques identified FCS as lysozyme c (Lzm-S), consistent with that originating from the canine spleen. Confirmatory tests showed that purified Lzm-S produced myocardial depression in the RVT preparation at concentrations achieved during sepsis in the in vivo preparation. In addition, Lzm-S inhibited the adrenergic response induced by field stimulation and the beta- agonist isoproterenol in in vitro preparations, these results suggesting that Lzm-S may inhibit the sympathetic response in sepsis. The present findings indicate that Lzm-S originating from disintegrating leukocytes from organs such as the spleen contributes to myocardial dysfunction in this model. The mechanism may relate to its binding or hydrolysis of a cardiac membrane glycoprotein thereby interfering with myocardial excitation-contraction coupling in sepsis.


Assuntos
Antagonistas Adrenérgicos/toxicidade , Infecções por Escherichia coli/etiologia , Coração/fisiopatologia , Muramidase/toxicidade , Choque Séptico/etiologia , Antagonistas Adrenérgicos/isolamento & purificação , Agonistas Adrenérgicos beta/farmacologia , Animais , Cães , Infecções por Escherichia coli/induzido quimicamente , Infecções por Escherichia coli/fisiopatologia , Contração Isométrica , Isoproterenol/farmacologia , Muramidase/isolamento & purificação , Contração Miocárdica/efeitos dos fármacos , Choque Séptico/induzido quimicamente , Choque Séptico/fisiopatologia , Baço/enzimologia , Trissacarídeos/farmacologia
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