FGF1ΔHBS ameliorates retinal inflammation via suppressing TSPO signal in a type 2 diabetes mouse model.

Translocator protein (18 kDa) (TSPO) plays an important role in retinal neuroinflammation in the early stage of diabetic retinopathy (DR). Studies have found that a FGF1 variant (FGF1ΔHBS) with reduced proliferative potency exerts excellent anti-inflammatory effects and potential therapeutic value for diabetic complications. In this study, intravitreal injection of FGF1ΔHBS was administrated every week for one month in db/db mice, which are genetically predisposed to develop type 2 diabetes mellitus and early retinopathy. Changes in retinal function and structure in the animal models were detected by electrophysiology (ERG) and optical tomography coherence (OCT). TSPO expression and retinal inflammation were analyzed by immunofluorescence, Western blot and real-time qPCR. In the retina of T2D (db/db) mice, FGF1 was significantly down-regulated while FGFR1 was up-regulated (both p < 0.05). TSPO and retinal inflammatory factors were all up-regulated. TSPO and FGFR1 were mainly co-stained in the inner retina. After FGF1ΔHBS treatment, ERG showed that the total amplitude of dark-adapted b-wave and oscillating potentials (Ops) was significantly improved, and OCT showed that the thickness of the retina around the optical nerve head was significantly preserved in T2D mice (all p < 0.05). The TSPO signal was significantly suppressed by FGF1ΔHBS. The activation of NF-κB p65 and the expression of inflammatory factors such as TNF-α, IL-1ß, IL-6, COX-2, MIP-1α, and iNOS were all significantly down-regulated (all p < 0.05). Collectively, our current data demonstrated that intravitreal FGF1ΔHBS treatment can effectively inhibit retinal inflammation via suppressing TSPO signal and to preserve retinal function and structure in a T2D mouse model.

Noncontact Intraocular Pressure Measurement over Bandage Contact Lens and the Effect of Pentacam and Corvis ST's IOP Correction System.

Purpose: To evaluate the influence of intraocular pressure (IOP) measurement while wearing bandage contact lens (BCL) and the effect of Pentacam and Corvis ST's correction systems. Methods: It was a prospective comparative study. Forty healthy subjects (40 eyes) were included in this study. Eyes were measured using noncontact tonometer (NCT), Corvis ST, and Pentacam before and after wearing BCL. Pentacam's five correction formulas (Ehlers formula, Shah formula, Dresden formula, Kohlhaas formula, Orssengo/Pye formula) and Corvis ST's correction formulas (Ehlers formula and biomechanical corrected formula) were used to correct the IOP values before and after BCL wearing. The IOP values were compared, and the correction effect of different systems were evaluated. Results: The mean age of the subjects was 24.4 ± 0.60 years. The mean IOP obtained by NCT was 14.8 ± 3.2 mmHg before BCL wearing and was 15.7 ± 3.4 mmHg after BCL wearing. The mean IOP was significantly increased after BCL wearing (0.9 ± 2.9 mmHg, P=0.05). Four of the five Pentacam's correction formulas (except Kohlhaas formula) showed no significant difference in the mean corrected IOP values before and after BCL wearing (all P > 0.05). The mean IOP obtained by Corvis ST was 13.7 ± 2.8 mmHg before BCL wearing and was 15.0 ± 4.0 mmHg after BCL wearing. The mean IOP was significantly increased after BCL wearing (1.3 ± 2.4 mmHg, P < 0.05). Corvis ST's correction formula (Ehlers formula other than biomechanical corrected formula) showed no significant difference in the mean corrected IOP values before and after BCL wearing (P > 0.05). Conclusion: The IOP measurements over BCL by NCT and Corvis ST was found to be increased. The correction systems of Pentacam (Ehlers formula, Shah formula, Dresden formula, and Orssengo/Pye formula) and Corvis ST (Ehlers formula) are useful in correcting the IOP measuring deviation induced by BCL wearing.