Histopathological classification and location of consecutively operated meningiomas at a single institution in China from 2001 to 2010 / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Meningioma is one of the most common primary tumors of the central nervous system, but there are not many detailed studies on the sex, age, subtypes and locations of large series. This study was a retrospective analysis of the characteristics of meningioma cases consecutively operated on at a single institution in China from 2001 to 2010.</p><p><b>METHODS</b>This study investigated the demographic background of 7084 meningioma cases, and the subtypes and locations of the tumors. Sex and age distributions were analyzed, and the pathological subtypes were classified according to the World Health Organization (WHO) classification. The location of the meningiomas was also categorized.</p><p><b>RESULTS</b>The female:male ratio of the 7084 cases was 2.34:1. The mean age was 51.4 years (range, 11 months-86 years). The mean age of cases of WHO grade I meningioma was significantly older than that of grade II or III meningiomas (P < 0.001, Fisher's Least Significant Digit test). There was a significantly higher female:male ratio in WHO grade I meningiomas than in grade II or grade III meningiomas (2.57, 1.03 and 0.76, respectively; P < 0.001, χ(2) test). Meningothelial (n = 2061) and fibrous meningiomas (n = 3556) were the most common subtypes, comprising 79.3% of all meningiomas. All meningioma cases were classified into 23 locations in this study, with the cerebral convexity the most common site (38.33%, n = 2722). Cases with uncommon locations such as extra-cranial and sylvian fissure meningiomas were also present in this series.</p><p><b>CONCLUSIONS</b>Female predominance was found for benign meningiomas, while malignant subtypes showed male predominance. The mean age of patients with WHO grade I meningiomas was older than that of patients with higher-grade tumors. Meningothelial and fibrous meningiomas were the most common subtypes. The cerebral convexity was the most common meningioma location.</p>

Bone marrow stromal cell transplantation for treatment of Parkinson's disease in rhesus monkeys / 中华神经医学杂志

Objective To evaluate the therapeutic effect of bone marrow stromal cell transplantation on Parkinson's disease (PD) in rhesus monkeys. Methods Experimental PD was induced in rhesus monkeys by stereotatic surgery to destroy the unilateral substantia nigra. Human bone marrow stromal cells were injected stereotaxically into the 9 target areas in the brain of the monkeys, after which behavioral test was performed and the alteration in the dopamine transporter (DAT) in the substantia nigra was examined using single-photon emission computerized tomography (SPECT). Immunofluorescence assay was employed to examine the TH-and BrdU-positive cells in the injured and contralateral substantia nigra. Results All the monkeys showed progressive deterioration of the PD symptoms after the cell transplantation. SPECT revealed no significant changes in DAT in the bilateral substantia nigra, but immunoflurescence assay showed that a proportion as high as 25% of the transplanted BMSCs differentiated into dopaminergic neurons. Conclusion Human BMSCs can not serve as the donor cells for treatment of PD in rhesus monkeys.

Analysis of cerebrospinal fluid protein expression in Parkinson's disease by two-dimensional difference gel electrophoresis / 中华神经医学杂志

Objective To identify differentially expressed proteins in cerebrospinal fluid (CSF) of Parkinson's disease (PD), so as to provide clues for investigating PD biomarkers. Methods Two-dimensional difference gel electrophoresis (2D DIGE) technique, in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), was used to determine the differentially expressed CSF proteins in PD patients in comparison with control subjects. Results The levels of 20 protein spots were significantly altered in PD CSF. Of them, 11 spots were up-regulated and 9 spots were down-regulated. Of the 8 proteins identified in the profile of differentially expressed protein spots between patients and controls, an isoform of apolipoprotein A-I, myosin phosphatase target subunit 1 (MYPT1), and 3 unknown proteins were down-regulated, whereas an apolipoprotein A-I isoform, proapolipoprotein, and lipoprotein were up-regulated. Conclusions MYPT1 is related with synapse function and proapolipoprotein, lipoprotein and apo A-I are associated with cholesterol metabolism. These proteins may have links with the pathogenesis of PD and may be identified as CSF biomarks in PD.

Diagnosis and treatment of dysembryoplastic neuroepithelial tumor / 中华外科杂志

<p><b>OBJECTIVE</b>To discuss the diagnosis and treatment of dysembryoplastic neuroepithelial tumor (DNT).</p><p><b>METHODS</b>From November 2001 to February 2005, 18 patients were admitted. The data of the 18 patients were reviewed.</p><p><b>RESULT</b>Epilepsy was the main complaint. There was no mass effect on MRI. Multinodular and specific glioneuronal element was typical in pathological examination, seizure could be controlled by operation.</p><p><b>CONCLUSIONS</b>DNT is benign tumor which could be treated by surgery, total removal of tumor and using intraoperative electrocorticography could improve the result of operation.</p>

Diagnosis of intracranial malignant melanoma / 中华外科杂志

<p><b>OBJECTIVE</b>To analyse the diagnosis points of intracranial malignant melanoma.</p><p><b>METHODS</b>There were ten patients with intracranial malignant melanoma (but without clear extracranial primary lesion), whose epidemiology, clinical symptom, physical sign and imaging data were analysed.</p><p><b>RESULTS</b>Most of patients were young men, The Clinical symptom or physical sign are similar to brain tumor or cerebral hemorrhage, but the course was shot, two cases of among having skin melanotic nevus. As for most of cases, CT were high density, MRI T1WI showed high signal and T2WI low signal, which often had hemorrhage. Histological examination showed all cases were malignant melanoma. Preoperative misdiagnosis was up to eight cases (80%).</p><p><b>CONCLUSIONS</b>For preoperative accurate diagnosis, this disease's epidemiology, clinical symptom, extracranial physical sign and imaging data points could be synthetical analysed.</p>

An allelotype study of primary and corresponding recurrent glioblastoma multiforme / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To investigate molecular genetic alterations associated with primary and corresponding recurrent glioblastoma multiforme(GBM) and to identify which chromosomal regions of the whole genome may be involved in the recurrence of primary GBM.</p><p><b>METHODS</b>A high-resolution allelotyping study of one patient's primary GBM and corresponding recurrent GBM was performed by PCR-based loss of heterozygosity(LOH) analysis with the use of 382 fluorescent dye-labeled polymorphic microsatellite markers covering all 22 autosomes. The mean genetic distance between two flanking markers is 10 cM.</p><p><b>RESULTS</b>LOH at locus D9S157 on 9p21 and at loci D10S537, D10S185, D10S192, D10S597, D10S587, D10S217 on 10q21.3-26.3 was observed in the primary GBM. As for corresponding recurrent tumor, LOH was observed not only in expanded regions on 9p21 and 10q21.3-26.3 but also on multiple other chromosomal arms, including 1q, 7p,7q, 21q, 20p, 20q, 10p, 19p, 19q.</p><p><b>CONCLUSION</b>Chromosome 9p and 10q may be involved in the development of this GBM. Although histopathological diagnoses of the primary and corresponding recurrent tumor are identical, the recurrence of GBM is characterized by an increased involvement of molecular genetic abnormalities and may be accompanied by inactivation of more tumor suppressor genes.</p>