Anti-tumor Effect and Mechanism of Effective Components in Hedysari Radix： A Review / 中国实验方剂学杂志

Malignant tumors are one of the main causes of human death worldwide and pose a serious threat to human health. The current treatment methods are mainly the combination of chemotherapeutics， surgery， radiotherapy， or hormone therapy. The treatment process has limitations such as multidrug resistance， non-selective targeting of cancer cells， and drug toxicity. With the development and application of traditional Chinese medicine （TCM）， Chinese medicine has the characteristics of multi-angle and multi-mechanism coordination and slight toxic and side effects. It can effectively inhibit tumor proliferation， differentiation， and metastasis， and avoid drug resistance， serving as the focus of current tumor treatment research. Hedysari Radix， one of the genuine medicinal materials in Gansu province， is a tonic Chinese medicine with a wide range of pharmacological effects such as anti-inflammation， immune regulation， anti-oxidation， prevention and treatment of diabetic complications. In the majority of the ancient works on herbs of the past dynasties， Hedysari Radix was included under the item of Astragali Radix and used as Astragali Radix. Hedysari Radix is superior to Astragali Radix in enhancing immunity， scavenging free radicals， and resisting liver fibrosis. Studies have found that the effective components of Hedysari Radix have a prominent anti-tumor effect and a significant inhibitory effect on various malignant tumors such as liver cancer， bladder cancer， gastric cancer， breast cancer， and colorectal cancer. They can also combine with clinical anti-cancer drugs to reduce the toxic and side effects of chemotherapy drugs and improve the tolerance of patients during chemotherapy. On the basis of current research， this study summarized the mechanism of Hedysari Radix active components in inducing cell apoptosis， blocking cell cycle， inhibiting tumor cell proliferation， migration， and invasion， regulating micro mRNA （miRNA）， inducing cell autophagy， enhancing immune regulation， as well as reducing toxicity and enhancing efficiency and sensitization with clinical chemotherapeutics， and systematically explained the anti-tumor mechanism of Hedysari Radix active components， aiming to provide a basic reference for the further exploration of the anti-tumor mechanism of Hedysari Radix and the further development and utilization of its effective components.

Optimization of the Extraction Technology of Total Flavonoids from the Roots and Stems of Angelica sinensis / 中国药房

OBJECTIVE: To optimize reflux extraction technology of total flavonoids from the roots and stems of Angelica sinensis. METHODS: The reflux extraction technology of total flavonoids from the roots and stems of A. sinensis was optimized by Box-Behnken design-response methodology based on single factor test using volume fraction of extraction solvent ethanol, solide-liquid ration, extraction time, extraction times as investigation factors, the content of total flavonoids in extract as evaluation index. RESULTS: The optimal extraction technology of total flavonoids from the roots and stems of A. sinensis was that volume fractions of ethanol were 70％ and 50％; solid-liquid ratios were 1: 40 and 1: 30; extraction time were 1. 3 h and 1. 7 h; The number of extraction times is two times. In verification test, the contents of total flavonoids were 7. 253 6, 25. 144 1 mg/g (RSD= 1. 57％, 1. 49％, n = 3); relative errors of those to predicted value (6. 942 8, 25. 703 5 mg/g) were 4. 28％, 2. 24％. CONCLUSIONS: Optimized extraction technology for total flavonoids from the roots and stems of A. sinensis is simple, reproducible and predictable.

Inhibition on proliferation of HeLa cells in vitro of nano sllicon equipped with 4'-demethylepi-podophyllotoxin / 药物评价研究

Objective To study the inhibitory effect on proliferation of Hela cells of podophyllotoxin,4'-demethylepi-podophyllotoxin and drug combination of different proportion of nano-sillca (SiO2) and 4'-demethylepi-podophyllotoxin in vitro,and discuss the mechanism.Methods Used ethyl silicate hydrolysis method to prepare 25 nm SiO2 sample,next carried 4'-demethylepi-podophyllotoxin after the surface modification,and measure cell campatibility by MTT method and Hoechst 33342.The inhibitory effect of podophyllotoxin,4'-demethylepi-podophyllotoxin and drug combination on proliferation of Hela cells was measured by MTT assay.Hoechst 33342 staining method was used to detect cell apoptosis.The effect ofdmg combination treatment on cell morphology was observed by inverted microscope.Western blotting technique was used to detected effect of 4'-demethylepi-podophyllotoxin and drug combination on expression of apoptosis related protein.Results Inhibitory effect onproliferation of Hela cells of 4'-demethylepi-podophyllotoxin is superior to podophyllotoxin,inhibitory effect of drug combination is superior to the single 4'-demethylepi-podophyllotoxin,the inhibition of drug combination with 0.125 μg/mL nano SiO2 and 6.25 μg/mL 4'-demethylepi-podophyllotoxin is the most obvious.MTT and Hoechst 33342 experimental results showed that the 25 nm SiO2 have good cell compatibility.Podophyllotoxin,4'-demethylepi-podophyllotoxin and drug combination can induce apoptosis.Western blotting results showed that 4'-demethylepi-podophyllotoxin and drug combination can up-regulate the ratio of Bax/Bcl-2 and the expression level of Caspase-3、P53 and P38.Conclusion In vitro experimental performance of drug combination is superior to single 4'-demethylepi-podophyllotoxin,it is may by effecting the expression of Bcl-2,Bax,Caspase-3,P53,and P38 and others apoptosis related protein to induce Hela cell apoptosis.

Analysis on Quality of Rheum Palmatum L. from Gansu Province Based on Multicriteria Method / 中国中医药信息杂志

Objective To compare the quality of Rheum palmatum L. in different areas and ages in Gansu Province by comparing five components in Rheum palmatum L. through fingerprints and QAMS (quantitative analysis of multi-components) methods; To determine the most suitable growing areas and the best development areas in Gansu Province. Methods The water content, total ashes, and water-soluble extract content in 15 batches of Rheum palmatum L. from different areas and ages in Gansu Province were determined according to the approaches listed in the Chinese Pharmacopeia. The contents of five index components were determined through HPLC and the quality of 15 batches of Rheum palmatum L were evaluated by fingerprint and QAMS method. Results The results from 15 batches of Rheum palmatum L. showed that the water content was less than 15％, total ashes less than 10％, and water-soluble extract content less than 25％, conforming to Pharmacopoeia. There were 23 common peaks in the fingerprints of 15 batches of Rheum palmatum L.. Five of them were identified as aloe-emodin, rhein, emodin, chrysophanol and physcion, which showed good linear relationship in the range of 0.0122–0.7344 μg (r=0.9999), 0.00714–5.7120 μg (r=0.9999), 0.0088–0.7040 μg (r=1.0000), 0.1224–3.6720 μg (r=0.9999) and 0.0148–5.9200 μg (r=0.9997), respectively. There was no significant difference between the calculated value and the measured value by using the relative correction factor (RCF), and the reproducibility of RCF was good. The quality of Rheum palmatum L. from different areas and ages in Gansu Province significantly differed (P<0.05). Two year old Rheum palmatum L. from Pingxiang village of Li county and Rushu village of Tanchang county had the best quality, and that of three years old was better than two years old from Tanchang county. Conclusion The established fingerprints and QAMS method is accurate, feasible, and can be used for the quality comparison of Rheum palmatum L. from different areas and ages in Gansu Province. Li County and Tanchang County areas in Gansu Province can be used as suitable planting areas and development industry. Therefore, it is recommended to select more than two yeas old Rheum palmatum L. from Tanchang County and Li County in Gansu Province for medicinal application.

Seasonal decomposition and ARIMA methods in prediction of tuberculosis incidence in Urumqi,China / 军事医学

Objective To compare the accuracy of the seasonal time series decomposition method and autoregressive integrated moving average (ARIMA) in the prediction of incidence of tuberculosis(TB) in order to facilitate early-warning.Methods The seasonal decomposition model and ARIMA model were constructed by SPSS20.0 software based on time series of monthly TB incidence between January 2005 and December 2014 in Urumqi,China.The obtained models were used to forecast the monthly incidence in 2015 and compared with the actual incidence respectively.Results Between 2005 and 2014,the incidence of TB was higher during March,April and May in Urumqi.A linear fitting model and a cubic curve fitting model were constructed by the time series seasonal decomposition method.The mean absolute percentage error (MAPE) of each predicted monthly incidence in 2015 was 18.75% and 92.25%,respectively.The predicted values of the linear model were lower than actual values and the predicted values of the cubic curve model were higher than actual values.An ARIMA (2,1,1) (1,1,0)12 fitting model was established by ARIMA method.The MAPE of each predicted monthly incidence in 2015 was 9.46% and there were no significant differences between the predicted and actual values.Conclusion The ARIMA method is better than the seasonal decomposition method for predicting the monthly incidence of TB in Urumqi.

Development of a new breathing control system of tumor target for precision radiotherapy / 中国医学装备

Objective:It is more difficult to conduct precision radiotherapy for organs of the human body with the respiratory movement. It is necessary to compensate a certain degree of deviation which is produced by many thoracic and abdominal organs with breathing exercises. To develop a new breathing control system of tumor target for precision radiotherapy is the practical demand in hospital.Methods: According to the current active breathing control system, there are gasbag, control box, handle switch for the patients, computer, automatic gasbag controller, respiratory sensor and communication tools. The Pneumotach PowerCube pulmonary function respiratory sensor and C++ high level programming language were selected to program the breathing control system.Results: The system could make patients conduct respiratory control better, reduce the deviation of pulmonary tumor caused by respiratory movement and improve the accuracy of treatment.Conclusion: The system had a lot of functions, such as dispersion, oscillation, ventilation and so on. It is refitted on the current instruments and successful to clinical application.

Study on Quality Evaluation of Hedysari Radix from Different Producing Areas in Gansu Province Based on the Contents of 12 Trace Elements / 中国中医药信息杂志

Objective To evaluate the quality of Hedysari Radix from different producing areas in Gansu Province by setting Hedysari Radix from different producing areas in Gansu Province as study subjects and trace elements in Hedysari Radix as evaluation indexes; To provide the references for establishment of the quality standards of Hedysari Radix.Methods The content of Fe, Cu, Ca, Mn, Zn, Mg, Cr, Co, Na, Li, Ni and K in Hedysari Radix were detected by using flame atomic absorbption method. Data were analyzed by the factor analysis conducted by SPSS21.0 software. The common factors were extracted by principal component and analytic function for quality comprehensive ranking was established.ResultsThree common factors were extracted, namely F1, F2, and F3. The quality function was Y=0.657F1+0.119F2+0.089F3. Cr, Li, Co, Cu, Zn, Fe and Mg were the characteristic trace elements of Hedysari Radix that could affect the quality of Hedysari Radix. When the quality of Hedysari Radix was evaluated by the contents of trace elements, the quality of wild Hedysari Radix were better than cultivated Hedysari Radix and the quality rank was consistent with the idea that Longnan is the main producing area of Hedysari Radix. The quality of samples from Dingxi area was better.Conclusion The quality evaluation of Hedysari Radix based on trace elements shows the differences in Hedysari Radix from different producing areas in Gansu Province and the results are relatively reasonable.

Comprehensive Quality Evaluation of Gansu Baitiao Codonopsis Radix with Different Commercial Grades / 中国中医药信息杂志

ObjectiveTo compare the quality of Baitiao Codonopsis Radix with different commercial grades; To provide the basis for the rational utilization of Codonopsis Radix.MethodsTotally 54 batchs of Baitiao Codonopsis Radix with different commercial grades from main producing areas in Gansu were collected. The contents of lobetyolin and atractylenoideⅢ in Codonopsis Radix were detected by HPLC. Colorimetric method was used to detect the content of polysaccharide in the Codonopsis Radix. Chinese Pharmacopoeia 2010 Edition was referred to detect the content of extractum. One way ANOVA and factor analysis method were used to comprehensively evaluate the data.ResultsLobetyolin and atractylenoideⅢ were all negatively correlated with commercial grades. The results of the principal component factor ranking showed that the samples were not sorted by the order of commercial grades, with commercial grade two ranking in the forefront.Conclusion The quality of Baitiao Codonopsis Radix with different commercial grades is different, and the quality of commercial grade two is better than other commercial grades.

Appliation of oncoplastic breast-conserving reconstruction for patients with early-stage breast cancer / 国际肿瘤学杂志

Objective To analyze the cosmetic effects and postoperative complications of patients with early breast cancer who underwent oncoplastic breast-conserving reconstruction and traditional breast-conserving surgery.Methods From January 201 2 to October 201 5,we collected a total of 67 patients with early-stage breast cancer who underwent breast-conserving surgery in Affiliated Mindong Hospital of Fujian Medical Univer-sity.Thirty patients who underwent oncoplastic breast-conserving reconstruction were in observation group,and 37 patients who underwent traditional breast-conserving surgery were in the control group.The postoperative complications,cosmetic effects and survival situations in the two groups were compared.Results In the aes-thetic effects evaluation,22 patients (73.33%)and 1 6 patients (43.24%)had good or excellent cosmetic effects,6 patients (20.00%)and 1 4 patients (37.84%)had general cosmetic effects,2 patients (6.67%) and 7 patients (1 8.92%)had poor cosmetic effects in the observation group and control group,with a signifi-cant difference (Z =-2.51 3,P =0.01 2).Four patients (1 3.33%)in the observation group had postopera-tive complications,including that 1 patients had incisional dehiscence followed by incisional wound infection and skin necrosis,and 3 patients had subcutaneous exudates.However,2 patients (5.41 %)in the control group had postoperative complications,including 1 patients with incisional dehiscence,and 1 patients with inci-sional wound infection.There were no significant difference in the occurrence rates of postoperative complica-tions in the two groups (χ2 =0.490,P =0.484).The mean follow-up period was 28 months.We found 1 patients of local recurrence,and 2 patients of metastases in the control group.However,all patients were free of recurrence and metastases in the observation group,with no significant difference (P =0.1 40).Conclusion Oncoplastic surgical technique in the breast-conserving surgery for patients with early-stage breast cancer is a safe and effective procedure,with highly satisfactory cosmetic effects for the majority of patients,which is worth to recommend.

Study on Characteristics of Trace Elements of Hedysari Radix in Different Growth Ages / 中国中医药信息杂志

Objective To discuss the correlativity of the characteristics of trace elements and the quality in Hedysari Radix in different growth ages by taking the Hedysari Radix with different growth ages in Gansu as the research objects. Methods Hedysari Radix in different growth ages was collected. The contents of 12 trace elements in Hedysari Radix were detected by using flame atomic absorptions pectrophotometer.The multiple comparisons, correlation analysis, discriminant analysis and factor analysis were performed by SPSS21.0. Results The contents of Ni, Mg, and Mn were significant in Hedysari Radix with different growth ages. The growth ages had significantly negative correlation with Zn contents and had significantly positive correlation with Li contents. The contents of Cu, Fe, Ca, K, Zn, Na, and Mn and growth ages existed Fisher linear. The quality of Hedysari Radix with different growth ages had difference;the quality of Hedysari Radix with 2-year-old growth ages also had difference; the quality of 3 and 4-year-old Hedysari Radix was better than 6 and 8-year-old. Conclusion There are closely relationships between growth and the contents of certain trace elements. The quality of Hedysari Radix has some relationships with the contents of trace elements.

Characteristic Analysis on Trace Elements from Hedysari Radix in Different Months / 中国中医药信息杂志

Objective To analyze the characteristic changes of trace elements from Hedysari Radix in different months;To provide references for the choice of the best harvest periods. Methods Trace elements of Hedysari Radix from different months (June, July, August, September, October and November) were determined by atomic absorption spectroscopy and analyzed by single factors analysis of variance, correlation analysis, cluster analysis and factors analysis. Results There was obvious significance between the contents of Cu and Co (P<0.05), and there was obvious significance among the contents of Fe, Ca, Mn, Zn, Mg, Cr, Na, Li, Ni and K (P<0.01). The results of correlation analysis showed that months had positive correlation with Cu, had a negative correlation with Fe, Na, Mn, Cr and Li contents, and had significantly negative correlation with Ca, Mg contents. Mn, Cr, Ni, K and Na were the characteristics of trace elements from Hedysari Radix in different months. The different months samples could be divided into two categories of June, July, August, September samples (Ⅰ) and October, November samples (Ⅱ). Conclusion There is close relationship between the contents of trace elements from Hedysari Radix and months. The information and metabolic patterns of trace elements from Hedysari Radix in June, July, August, September (immature) samples are close or similar;the information of trace elements in October, November (nearly mature or maturing) samples are close or similar. Study on harvest times of Hedysari Radix by trace element as an indicator is with certain rationality.

Study on the Correlation between Contents of Active Ingredients in Hedysarum polybotrys from Gansu and Ecological Factors / 中国药房

ABSTRCT OBJECTIVE:To analyze the correlation between contents of active ingredients in Hedysarum polybotrys and ecologi-cal factors. METHODS:HPLC was used for the simultaneous determination of calycosin and formononetin in H. polybotrys;UV spectrophotometry was used to determine the content of hedysari polysaccharide;and hot-dip method was used to determine the con-tent of extracts in H. polybotrys. Factor analysis method was used to analyze the correlation between contents of active ingredients and ecological factors. RESULTS:4 common factors,which were heat,geography,illumination and precipitation,were extracted. The heat was an ecological dominant factor influencing the quality of H. polybotrys. And ≥10 ℃ accurnulated temperature was the most significant variate influencing hedysari polysaccharide,latitude was the most significant variate influencing the contents of ca-lycosin and formononetin H. polybotrys;and annual averge amount of radiation was the most significant variate influencing content of extracts in H. polybotrys. CONCLUSIONS:Different ecological factors have different effects on the contents of active ingredi-ents in H. polybotrys,and it is the result of the combined effect of various of ecological factors.

Epidemiological investigation and analysis of an outbreak caused by Mycoplasma pneumoniae / 军事医学

Objective To investigate the epidemiological characterization , effect of prevention and control measures during an outbreak in a military unit caused by Mycoplasma pneumoniae.Methods All the cases were investigated to learn about their basic characteristics , clinical symptoms and aggregation activities using epidemiological cross-sectional study . Results From May 16 to June 11,2013, forty-nine confirmed cases and thirty-five suspected cases who were all males were found at an attack rate of 7.8%.The epidemic peak period was from May 27 to Jun 2(seven days), and the number of total cases was 66(75.9%).During the outbreak, the attack rate of the 10th cadet team, other cadet teams and a non-student population was 47.5%, 4.5% and 1.2% respectively.The difference was significant (P<0.01).The age of forty-nine confirmed cases ranged from 17 to 26 years old , the attack rate did not differ significantly between different age groups, and no aggregation was found in the place of birth and soldier source .The most frequent clinical symptoms were fever and cough for the confirmed cases , 62.5% of whom showed unilateral or bilateral lung inflammation change , while few positive lung symptoms were present .All the confirmed cases were hospitalized and no severe or critically ill cases were present.The outbreak was controlled effectively after implementation of case surveillance , contact precaution , termination of aggregation activities , dispersed habitation and prescription of azithromycin for disease prevention .Conclusion The confined and crowed environment for learning and training can increase the risk of M.pneumoniae outbreak , making it nec-essary to enhance disease prevention awareness , improve case management , live dispersed , reduce close contact and imple-ment personal hygienic measures .

Quality Standard of Radix Hedysari / 中国药房

OBJECTIVE: To study the quality standard of Radix Hedysari from Gansu province. METHODS: Radix Hedysari was analyzed in respects of its origin, property, microscopic characteristics, physicochemical property, TLC identification, moisture, determination of ash and determination of ethanol-soluble extractives etc. RESULTS: The results showed that the Radix Hedysari from Guansu was of high quality and in which, few counterfeit products was noted. CONCLUSION: In this study, a systematic evaluation for the Radix Hedysari from Gansu province was achieved, and it provides a scientific evidence for the quality control Radix Hedysari from Gansu province and guarantee of its genuine property.

The Hormone Ingredient in the Egg-Oil of Chinese Woodfrog (Rana temporaria chensinensis) and the effect of Egg-Oil on Platelet Aggregation and blood Lipid / 中草药

Hormone ingredient in the egg oil, egg and Oviductus Ranae of Rana temporaria chensinensl is David were studied as well as the effect of egg-oil on platelet aggregation and blood lipid.Results proved that the estradiol content in the egg-oil is very high.The egg-oil can obviously inhibit blood platelet aggregation and showed an hypolipemia activity. It is expeetedthat the egg-oil of R. temporaria chensinensis can be developed as an efficient medicine for the prevention and cure of atherosclerosis and hyperlipemia in elderly.