Feasibility study of Shuyisha as hemostasis and repair material for liver wound / 中华创伤杂志

Objective To discuss the feasibility of Shuyisha as hemostasis and repair material for liver wound. Methods Hemolysis rate, acute toxicity and eytotoxicity of Shuyisha were measured. A hemorrhage model was established by making an open wound (5 mm× 3 nun ×2 mm) on the left liver lobe of mice. Hemostasis was performed with Shuyisha in experimental group and with Surgicel in control group, when the hemostatic time and total blood loss (TBL) were accurately recorded and regular macro-scopic and histological observation carried out. Results The hemolysis rate of Shuyisha was 2.33%, with maximum tolerance does of over 0.48 g/kg and the eytotoxicity at zero. The hemostatie time of Shuy-isha was (5.00 ±0.00) s, with total blood loss of (0.88±0.18) g/kg, better than Surgicel (P< 0.05). Shuyisha was degraded completely within 14 days, with the wound healed within 21 days in ex-perimental group, much better than Surgieel. Conclusions The hemolysis rate, acute toxicity and cy-totoxicity of Shuyisha are up to the requirement of biomedical materials. Shuyisha has effective hemosta-sis, which may be related to its molecular structure and adhesion.

The antioxidative and immunostimulating properties of D-glucosamine.

The objective of the present study was to investigate the antioxidant activity and immunostimulating property of glucosamine (GlcN) using various in vitro and in vivo tests. Results showed that GlcN possessed excellent antioxidant activities as manifested by strong chelating effect on ferrous ions and protection of macromolecules such as protein, lipid, and deoxyribose from oxidative damage induced by hydroxyl radicals. The immunostimulating effects of GlcN were further evaluated through various immunological tests. GlcN showed excellent activity of enhancing splenocyte proliferation. Neutral red pinocytosis and NO production in mouse peritoneal macrophages were significantly augmented. Oral administration of GlcN to mice for 20 days significantly enhanced the serum antibody level in mice in response to sheep red blood cells (SRBC), increased the relative organ weight of spleen and thymus tissue, and promoted the delayed-type hypersensitivity (DTH) against SRBC as compared with control group. In conclusion, the present investigation reveals GlcN is biologically functional in antioxidative activities and immunostimulating properties.

Protective effects of chitosan oligosaccharide and its derivatives against carbon tetrachloride-induced liver damage in mice.

The protective effects of chitosan oligosaccharide (COS), d-glucosamine (GlcNH(2)) and N-acetyl-d-glucosamine (GlcNAc) on carbon tetrachloride (CCl(4))-induced hepatotoxicity and the possible mechanisms that involved were investigated in male ICR mice. CCl(4) (20mg/kg body weight, i.p.) administration induced marked increase in serum AST and ALT activities, primed liver lipid peroxidation, depleted sulfhydryl content, impaired total antioxidant capabilities and induced genotoxicity 24h after administration. Pretreatment with COS, GlcNH(2), and GlcNAc (1.5g/kg body weight, i.g.) for 12 consecutive days prior to CCl(4) challenge significantly induced metallothionein (MT) expression. Thus, the antioxidant defensive system in the body was strengthened to counteract the oxidative damage induced by the succedent CCl(4) administration. Serum AST and ALT activities were effectively decreased. Hepatic malondialdehyde formation was inhibited and sulfhydryl contents, total antioxidant capabilities were markedly restored. Genotoxicity as reflected by DNA fragmentation, however, was not mitigated by pretreatment with COS, GlcNH(2), and GlcNAc. Histophathologic results of liver also confirmed their hepato-protective effects. Pretreatment with COS, GlcNH(2), and GlcNAc also could significantly decrease serum creatinine and uric acid levels and inhibit lipid peroxidation in kidney homogenate. Our results suggest that pretreatment with COS, GlcNH(2), and GlcNAc can efficiently protect mice against CCl(4)-induced toxicity.

Serum soluble E-cadherin level in patients with endometriosis / 中国医学科学杂志(英文版)

<p><b>OBJECTIVE</b>To investigate the serum sE-cadherin level in patients with endometriosis and the alterations of that level in healthy control during the menstrual cycle.</p><p><b>METHODS</b>Thirty-two patients with endometriosis and 30 healthy women were tested for serum sE-cadherin levels by enzyme-linked immunosorbent assay.</p><p><b>RESULTS</b>The serum sE-cadherin levels in healthy control did not vary throughout the menstrual cycle, which were lower than those in patients with endometriosis.</p><p><b>CONCLUSIONS</b>E-cadherin might be involved in endometrial shedding during menstruation in endometriosis patients. The serum sE-cadherin assay might be helpful as a serum marker for the diagnosis and management of endometriosis.</p>

EFFECTS OF GLUCOSAMINE ON IMMUNE FUNCTION IN MICE / 营养学报

Objective:To study the effects of glucosamine(GlcNH2) on immune function in mice.Method:The effects of GlcNH2 on murine proliferation of splenocytes were carried out in vitro.After feeding mice by GlcNH2,the phagocytotic functions of mononuclear macrophage,murine delayed type hypersensitivity(DTH) caused by sheep red blood cells(SRBC),the ability of antibody production(tested by HC50),and the index of immune organs(thymus and spleen) were deteimined in vivo.Results:GlcNH2 could promote the proliferation of splenocytes,phagocytotic functions of mononuclear macrophage,DTH,the ability of antibody production and the index of immune organs.Conclusion:Glucosamine can enhance immune function in mice such as cellular immunity,humoral immunity and non-specific immunity.