RESUMO
Plasma levels of meprin A, IL-6, and IL-18 were measured in 68 patients with acute decompensated heart failure at the time of admission to the hospital and after 1 year. The patients were assigned to groups depending on renal function disorder which was assessed by glomerular filtration rate (GFR). During hospital stay, the plasma levels of meprin A in patients with normal GFR (≥90 ml/min/1.73 m2) were considerably higher than in patients with reduced GFR (<90 ml/min/1.73 m2): 1.80 (0.86; 2.65) and 1.04 (0.56; 1.60) ng/ml, respectively. The levels of IL-6 and IL-18 did not differ significantly. After 1 year, plasma levels of meprin A and interleukins markedly decreased in patients with normal GFR (0.33 (0.20; 0.86) ng/ml) and remained high in patients with reduced GFR (0.92 (0.39; 1.33) ng/ml). Thus, the dynamics of meprin A levels in patients with acute decompensated heart failure depends on functional state of the kidneys, which may affect the course of heart failure.
Assuntos
Insuficiência Cardíaca , Rim , Metaloendopeptidases , Humanos , Insuficiência Cardíaca/sangue , Insuficiência Cardíaca/diagnóstico , Insuficiência Cardíaca/fisiopatologia , Interleucina-18/sangue , Interleucina-6/sangue , Metaloendopeptidases/sangue , Admissão do Paciente , Tempo de Internação , Taxa de Filtração Glomerular , Rim/fisiopatologiaRESUMO
We studied the effects of direct intratracheal and indirect intravenous administration of pathogen on the concentrations of types I, III, and IV collagens and fibronectin and expression of the corresponding genes at the early stages of LPS-induced lung injury. Direct and indirect administration of LPS increased the level of type III collagen and decreased the expression of genes encoding types I and III collagens. Expression of type IV collagen remained unchanged. Considerable decrease in fibronectin gene expression was observed after direct LPS administration. Thus, changes in the extracellular matrix of the lungs at the early stages of acute lung injury differ depending on the way of pathogen administration.
Assuntos
Lesão Pulmonar , Animais , Colágeno/metabolismo , Matriz Extracelular/metabolismo , Fibronectinas/metabolismo , Pulmão/metabolismo , Lesão Pulmonar/induzido quimicamente , Lesão Pulmonar/genética , Lesão Pulmonar/metabolismo , RatosRESUMO
We studied modulation of the expression of extracellular matrix proteins under conditions of meprin inhibition in rats with LPS-induced endotoxemia. Endotoxemia increased the expression of type I, III, IV collagens and fibronectin in the renal tissue and type III and IV collagens in the heart. Meprin inhibitor actinonin reduced expression of both meprins and genes of extracellular matrix proteins, but the intensity of this effect in the heart and kidney was different. Inhibition of meprins in endotoxemia can prevent pathological remodeling of the extracellular matrix in the heart and kidney.
Assuntos
Endotoxemia/metabolismo , Proteínas da Matriz Extracelular/metabolismo , Rim/metabolismo , Animais , Colágeno/metabolismo , Ácidos Hidroxâmicos/metabolismo , Metaloendopeptidases/metabolismo , Miocárdio/metabolismo , RatosRESUMO
Soluble receptor of IL-6 (sIL-6R) and antagonist of the receptor complex, soluble glycoprotein 130 (sgp130) mediate opposite effects during inflammation. We measured the levels of these cytokines and their ratio in rat blood on the model of acute lung injury. The injury was modeled by the intratracheal administration of LPS. The levels of sgp130 and sIL-6R increased during the inflammatory process in the injured lungs. The sgp130/sIL-6R ratio increased or decreased depending on the intensity of the inflammatory process. sgp130/sIL-6R ratio might reflect the intensity of inflammation during lung injury.
