RESUMO
The influence of mitochondria-targeted antioxidant 10-(6-plastoquinonyl)decyltriphenylphosphonium (SkQ,) different concentrations on respiratory parameters and lipid peroxidation process intensity in the rat liver after hypothermic storage was investigated. It was shown that cold storage of isolated liver during 18 h results in accumulation of TBA-active products, increase of intensity of Fe(2+)-ascorbate induced lipid peroxidation and depression of mitochondria functional activity. SkQ1 inhibits activation of free radical processes in a dose-dependent manner and it has a positive influence on energetic coupling in mitochondria.
Assuntos
Antioxidantes/farmacologia , Temperatura Baixa , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Mitocôndrias Hepáticas/efeitos dos fármacos , Plastoquinona/análogos & derivados , Animais , Respiração Celular/efeitos dos fármacos , Metabolismo Energético/efeitos dos fármacos , Feminino , Fígado/metabolismo , Mitocôndrias Hepáticas/metabolismo , Técnicas de Cultura de Órgãos/métodos , Consumo de Oxigênio/efeitos dos fármacos , Plastoquinona/farmacologia , Ratos , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismoRESUMO
The influence of oxidative phosphorylation uncoupler 2,4-dinitrophenol (DNP) presence in preserving solution on the rat liver respiratory activity and ATP content after 18 h of hypothermic storage (HS) and following normothermic reperfusion (NR) was investigated. DNP presence on the HS stage led to decrease of ATP level as compared with the control. After DNP removal during NR the gradual recovery of oxidative phosphorylation coupling occurred. This fact resulted in improvement of mitochondrial functional state (V4 respiration rate decrease, respiratory control and ATP level increase).
