RESUMO
Plasmids have been constructed which contain genes coding for fused proteins including beta-galactosidase or human leukocyte interferon alpha 2 and monomeric or pentameric form of the main antigenic determinant of the foot-and-mouth disease virus (FMDV) serotype 01K. Expression of the hybrid genes has been studied. It is shown that fused proteins, containing beta-galactosidase and the antigenic determinant (monomer or pentamer), interact specifically with anti-FMDV anti-sera and with antibodies against peptide 141-160 of FMDV VP1 coat protein.
Assuntos
Aphthovirus/genética , DNA Recombinante , Epitopos/genética , Plasmídeos , Sequência de Aminoácidos , Aphthovirus/imunologia , Sequência de Bases , Interferon Tipo I/genética , Dados de Sequência Molecular , Proteínas Recombinantes de Fusão/genética , beta-Galactosidase/genéticaRESUMO
RecA-independent recombinations accompanying the processes of plasmids preparation and cloning into Escherichia coli cells are induced within the short direct and inverted repeats of several types.
Assuntos
Clonagem Molecular , Recombinação Genética , Sequência de Bases , DNA Recombinante , Escherichia coli/genética , Dados de Sequência Molecular , PlasmídeosRESUMO
Multicopy plasmids of pMCR series have been constructed from pRRN2 (a pBR322 derivative) including plasmids carrying transcription terminators downstream the tet gene and those with opposite orientation of the tet and RNAI genes. The plasmids permit cloning and high expression of genes with strong promoters.
