Non-invasive and rapid diagnosis of type 2 diabetes mellitus based on the analysis of hair by front-face fluorescence spectroscopy.

Rapid and early detection of pathologies for screening purposes is not always easy with traditional methods. New low-cost optical techniques that are suitable for preventive detection and, more specifically, for developing countries must be developed. The objective of this work is to propose UV-VIS fluorescence of hair as a promising technique for a simple and rapid preliminary diagnosis of type II diabetes. In this work, we analyzed 130 hair samples taken from volunteers of different ages at the Habib Thameur Hospital in Tunis, Tunisia. These samples were analyzed clinically beforehand, and 50 were classified as healthy acting as a control, 24 were classified as low-level diabetics with a glycated hemoglobin A1C (HbA1c) <7%, and the other 56 were classified as high-level diabetics having an HbA1c >7%. The fluorescence of the patients' hair led to several interesting results. Indeed, a clear discrimination was obtained not only between the healthy and patients with a disease, but also a discrimination between diabetics with low levels and high levels or diabetics with and without complication. Also, a strong correlation between fluorescence spectra and glycated HbA1c for the diabetic population was clearly established. A linear discriminant analysis shows that it is possible to predict the status of test patients after having trained a fraction of the population. All these results show the ability of the front-face fluorescence (FFF) technique to detect disease and predict advanced states simply by capturing the fluorescence of the hair illuminated by a single LED. This work shows for the first time, to our knowledge, the capability of the FFF technique on hair samples for the diagnosis of diabetes.

Fast diagnostic of osteoporosis based on hair analysis using LIBS technique.

Currently, the reference method for the diagnose of the osteoporosis pathology is the Dual energy X-ray Absorptiometry (DXA) technique which assesses Bone Mineral Density (BMD). As a major public health problem, classified as such by the World Health Organization, it is necessary to develop new techniques that are more accessible, especially for developing countries. The objective of this work is to propose a new technique based on laser-induced breakdown spectroscopy (LIBS) using calcium and sodium in hair as a biomarker for the detection of osteoporosis pathology. The study involved 90 women aged 50 to 70 years divided in three groups, thirty women with osteoporosis, thirty women with osteopenia and thirty healthy women. The application of LIBS technique on their hair to correlate calcium and sodium lines with T-scores allows a quick and cheap screening of the disease. The results showed strong correlations between calcium lines and disease. We noted a negative correlation between the intensity of calcium and sodium lines. In addition to these results, we show that we were able to cluster the three groups using the emission lines of these two elements. All these results support the idea that the use of LIBS applied to hair to evaluate calcium and sodium elements as effective biomarkers for the detection of osteoporosis is a promising approach.

[The role of piezosurgery in the treatment of a rare case of superinfected cemento-osseous dysplasia caused by actinomyces: a case report]. / L´intérêt de la piézochirurgie dans le traitement d'une dysplasie cémento-osseuse surinfectée par actinomycose: à propos d'un cas.

Cemento-osseous dysplasia is a benign fibro-osseous lesion affecting the alveolar bone. It is classified into three forms: periapical, focal or florid dysplasia. It is often asymptomatic and fortuitously discovered during a routine radiological examination. However, it may become symptomatic after superinfection, after patient's exposure to oral bacterial flora. We here report a case of florid cemento-osseous dysplasia associated with actinomycosis of bone in a 53-year-old Tunisian woman. This superinfection has been rarely reported in the literature; Boolean Searching on PubMed for the keywords "cemento-osseous dysplasia AND actinomyces" displays a single article (Smith et al. 2011). The treatment of actinomycosis infection often requires long-term antibiotic therapy, sometimes associated with surgical debridement, as in the case of this patient who underwent piezosurgery to treat dysplasia and necrotic bone.

Simple and fast diagnosis of osteoporosis based on UV-visible hair fluorescence spectroscopy.

The aim of this work is to propose a new analytical technique based on UV-visible fluorescence as an alternative to x-ray absorptiometry for diagnosing osteoporosis, as well as its early stage by identifying osteopenia. The equipment used consists of very cheap equipment compared to x-ray scanners with an LED as the excitation source. In this work, we analyzed 90 hair samples taken from volunteers of different ages at Habib Thameur Hospital in Tunis. These samples were previously analyzed by bone mineral density (BMD) and correspond to people suffering from osteoporosis and osteopenia, as well as healthy people. The main idea of this study is to show the correlation between the deficiency of bone density given by BMD and the variations of the relative concentrations of molecules present in the hair causing the changes observed in the fluorescence spectra. The results extracted from the spectra show a clear discrimination between healthy and sick patients and a very good correlation, in a Spearman sense, between the spectral measurements and the BMD's data for the patients with osteoporosis. In addition, we have isolated the main spectral region responsible for this correlation, which facilitates the identification of the molecule concentrations related to the decrease in BMD.

Muscular hypotonia as an onset manifestation of Tuberculosis meningitis in an HIV-negative patient.

Muscular hypotonia is considered as one of the rarest forms of initial onset signs of TBM, in addition to aphasia and hyponatremia, the awareness of those rare onset signs, a well-conducted diagnostic approach and early treatment can improve the outcome.

Expression profile of biomarkers altered in papillary and anaplastic thyroid carcinoma: Contribution of Tunisian patients.

AIMS: The objective of this study was to compare the protein expression profile between well-differentiated (papillary) and undifferentiated (anaplastic) thyroid carcinoma in Tunisian patients. METHODS: This first Tunisian retrospective study concerned data of 38 thyroid cancer cases (19 papillary carcinoma PTC and 19 anaplastic carcinoma ATC) collected at Salah Azaiez Institute of Tunisia. Immunohistochemistry was used to evaluate tumor expression of different molecular markers (p53, Ki67, E-cadherin, cyclin D1, bcl2, S100 and Her-2). The molecular expression was correlated with the clinicopathological characteristics of the tumors. RESULTS: There were 6 differentially expressed markers when comparing anaplastic thyroid carcinoma ATC with papillary thyroid carcinoma PTC. Expression of p53 and Ki67 were significantly increased in 16 and 18 ATC cases respectively, the Ki67 expression was lost in PTC. Cyclin D1, E-cadherin, bcl2 and S100 were overexpressed in PTC tumors; however, they were significantly decreased in ATC. The last marker, Her-2 was expressed in one case of PTC only. CONCLUSION: Our results, similar with findings of other ethnic groups, showed alteration in expression of molecular markers associated with tumor dedifferentiation, indicating loss of cell cycle control with increased proliferative activity in ATC carcinoma. These data support the hypothesis that ATC may derive from dedifferentiation of preexisting PTC tumor.