[The mechanisms of stimulation of cellular proliferation under the effect of L-amino acids in the tissue culture in young and old rats].

The statistical method of range correlation was used to analyse the correlation of individual characteristics of L-amino acids, as well as of the dipole moments of the first pair of the nucleotides of their codons to the biological activity of the amino acids, i.e. to the stimulation of the cellular proliferation. The experimental material was obtained in the organotypic tissues of ecto- and mesodermal genesis in young and old rats. It was demonstrated, that each of stimulating amino acids acts in the explants from old rats to 1,5-6 times rarely, as compared to the explants from young rats. The weak correlation was detected between the stimulation of the cellular proliferation and physical-chemical characteristics - hydrophobicity, dipole moment and isoelectrical point of the amino acids, - in many tissues, but high and moderate correlation was detected in the brain subcortical explants, cerebella and skin. However, the moderate close correlation was detected between the dipole moment of the first pair of the nucleotides of their codons and the stimulating activity for the explants of the brain subcortical structures (young and old rats), of cerebella and skin (young rats), Spearman correlation coefficient rS =0,52; 0,45; 0,60, 0,71 accordingly. The moderate close correlation was detected also for the explants of testis and cartilage. It can be suggested, that some physical-chemicaland biochemical characteristics of amino acids should correlate to their biological activity, especially in relation to the ectodermal tissue.

[Blood group-specific variability of sialic acid contents in the chorionic gonadotrophin structure]. / Gruppospetsificheskie variatsii soderzhaniia sialovykh kislot v strukture khorionicheskogo gonadotropina.

Low-pressure ion-exchange chromatography was used for isolation of chorionic gonadotropin (hCG) from urine of pregnant women. The purified hCG-preparations from various blood group donors were shown to differ in their sialic acid content. The highly purified hCG-preparations after gel chromatography were analyzed in the reaction of hemagglutination and the preliminary results suggest their blood group specificity.

[Affinity chromatography of nucleoprotein complexes]. / Affinnaia khromatografiia nukleoproteinovykh kompleksov.

Affinity sorbent containing cellular membranes of the cerebral cortex was obtained by means of an inclusion into porous matrix of polyacrylonitrile. Sorption of model and natural nucleoprotein complexes on the obtained sorbent was studied by the method of affinity chromatography. Distribution coefficients were determined. Natural nucleoprotein complexes isolated from brain possess a greater selectivity of binding to the cellular membranes of the cerebral cortex than that from liver.

[Preparative isolation of gonadotropin and urokinase on carboxylic cation exchangers]. / Preparativnoe vydelenie gonadotropina i urokinazy na karboksil'nykh ka tionitakh.

Low-pressure ion-exchange chromatography was used for sequential isolation of chorionic gonadotropin and urokinase (EC 3.4.99.26) from urine of pregnant women. Theoretical analysis of electrostatic interactions of protein macromolecules with the sorptive surface of a carboxylic cation exchanger was performed. This analysis allowed us to optimize the pH values for selective sorption and reversible desorption of the target component on a cation exchanger with a selected acidity of functional groups. The use of carboxylic cation exchangers KM-2p and Biokarb-GM allowed us to obtain the preparations with purity not inferior to commercially available preparations.

[Measuring neuraminidase activity by the thiobarbiturate method]. / Izmerenie aktivnosti neiraminidazy tiobarbiturovym metodom.

A number of reducing agents used for removing the excess of periodate in the reaction of the neuraminidase (EC 3.2.1.18) activity measuring by the thiobarbituric acid technique were compared. The toxic reagent sodium arsenite may be replaced by a 20% solution of ascorbic acid. The modified technique of the neuraminidase activity determining can be used in case of both high-molecular weight substrate ovomucin and low-molecular weight substrate-glycomacropeptide from milk whey.

[The use of a glycomacropeptide of kappa-casein as a substrate for neuraminidase]. / Ispol'zovanie glikomakropeptida kappa-kazeina v kachestve substrata neiraminidazy.

Glycomacropeptide from milk was studied; its molecular mass and amino acid composition were evaluated. When the glycomacropeptide was used as a substrate of neuraminidase, it exhibited many advantages as compared with ovomucin (high molecular substrate of the enzyme) due to increased content of sialic acids in the glycomacropeptide composition and to high solubility.

[Structure and sorption properties of regenerated hemosorbent SCN]. / Struktura i sorbtsionnye svoistva regenerirovannogo gemosorbenta SKN.

Stepwise desorption of protein components from the hemosorbent SCN after hemoperfusion was studied, and their molecular weights were determined. It was found that hard alkaline regeneration and oxidation with nitric acid do not affect the hemosorbent structure, composition and potentiometric characteristics. The regenerated hemosorbent can be successfully used for adsorbtion of extraneous low- and medium-molecular weight components to clarify microbiological liquids.

[Method of applying colchicine to the rat vagus nerve for the purpose of selective action on axonal transport]. / Metod applikatsii kholkhitsina na bluzhdaiushchii nerv krysy s tsel'iu izbiratel'nogo vozdeistviia na aksonal'nyi transport.

A method of local application of colchicine deposited in a wax and paraffin alloy to the nerve is suggested. The doses of 25, 50, 75 and 100 micrograms were tested. Four days after application of 75 and 100 micrograms of colchicine, light microscopy demonstrated a non-uniform thickening of neurofibrils and the increase in their argyrophilic properties. Electron microscopy discovered the reduced number of microtubules, disordered arrangement of neurofilaments and accumulation of the latter ones beneath the neurolemma. The overall electric activity of the nerve was not disturbed. The method of colchicine application in vivo ensures the prolonged action of the drug without nerve compression or injury, thereby enabling one to assess consistently the sequels of the blockade of neurotrophic factors for metabolism of the innervated structures.