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1.
J Radiol Prot ; 33(1): R1-16, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23296029

RESUMO

The assessment and management of risks associated with exposures to ionising radiation are defined by the general radiological protection system, proposed by the International Commission on Radiological Protection (ICRP). This system is regarded by a large majority of users as a robust system although there are a number of dissenting voices, claiming that it is not suitable for estimating the risks resulting from internal exposures. One of the specific issues of internal exposure involves short-range radiations such as Auger and beta particles. Auger- and beta-emitting radionuclides can be distributed preferentially in certain tissue structures and even in certain cellular organelles, according to their chemical nature and the vector with which they are associated. Given the limited range of the low-energy electrons in biological matter, this heterogeneous distribution can generate highly localised energy depositions and exacerbate radiotoxic responses at cellular level. These particularities in energy distribution and cellular responses are not taken into account by the conventional methods for the assessment of risk.Alternative systems have been proposed, based on dosimetry conducted at the cellular or even molecular level, whose purpose is to determine the energy deposition occurring within the DNA molecule. However, calculation of absorbed doses at the molecular level is not sufficient to ensure a better assessment of the risks incurred. Favouring such a microdosimetric approach for the risk assessments would require a comprehensive knowledge of the biological targets of radiation, the dose-response relationships at the various levels of organisation, and the mechanisms leading from cellular energy deposition to the appearance of a health detriment. The required knowledge is not fully available today and it is not yet possible to link an intracellular energy deposition to a probability of occurrence of health effects or to use methods based on cellular dosimetry directly.The imperfections of the alternative approaches proposed so far should not discourage efforts. Protection against exposure to Auger and low-energy beta emitters would benefit from mechanistic studies, dedicated to the study of energy depositions of the radionuclides in various cellular structures, but also from radiotoxicological studies to define the relative biological effectiveness of the various Auger emitters used in medicine and of certain low-energy beta emitters, whose behaviour may depend greatly on their chemical form during intake. The scientific expertise, as well as the human and physical resources needed to conduct these studies, is available. They could be now mobilised into international low-dose research programmes, in order to ultimately improve the protection of people exposed to these specific radionuclides.


Assuntos
Exposição Ambiental/análise , Lesões por Radiação/etiologia , Lesões por Radiação/fisiopatologia , Monitoramento de Radiação/métodos , Proteção Radiológica/métodos , Radioisótopos/efeitos adversos , Medição de Risco/métodos , Animais , Partículas beta , Humanos , Lesões por Radiação/prevenção & controle , Projetos de Pesquisa/tendências , Medição de Risco/tendências
2.
Int J Radiat Biol ; 83(10): 687-97, 2007 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17729163

RESUMO

PURPOSE: To study the effect of hydration level and plasmid packing on strand break induction in DNA by ultrasoft X-ray. MATERIALS AND METHODS: Bluescript (pBS, tight packing) and pSP189 (pSP, loose packing) plasmids were irradiated by 250, 380, and 760 eV ultrasoft X-rays at the Laboratoire pour l'Utilisation du Rayonnement Electromagnétique synchrotron facility (Orsay, France). Single and double strand breaks (SSB and DSB) were quantified by gel electrophoresis. RESULTS: The number of DSB per Gray and per Dalton in pBS plasmids were (5.6 +/- 0.1), (6.3 +/- 0.1) and (8.5 +/- 0.4)x10(-12) at 250, 380 and 760 eV, respectively. They were respectively 1.4 +/- 0.1, 1.1 +/- 0.1 and 1.9 +/- 0.2 times larger for pSP plasmids. SSB/DSB ratios varied between 4.4 and 6.4. CONCLUSION: The observed dependency of strand break induction by ultrasoft X-rays on the hydration level of DNA in plasmids films may be associated with: (i) Damage transfer from the water shell to the DNA and/or (ii) change in packing. 760 eV photons which are more often absorbed in the hydration shell and yield longer range electrons than 250 and 380 eV photons, induce more DSB per Gray and per Dalton, especially for the looser plasmid (pSP).


Assuntos
Dano ao DNA/efeitos da radiação , DNA de Cadeia Simples/efeitos da radiação , Plasmídeos/efeitos da radiação , Água/química , Raios X , Relação Dose-Resposta à Radiação , Eletroforese em Gel Bidimensional/métodos , Fótons
3.
Radiat Res ; 163(2): 222-31, 2005 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-15658899

RESUMO

TILDA, a new Monte Carlo track structure code for ions in gaseous water that is valid for both high-LET (approximately 10(4) keV/microm) and low-LET ions, is presented. It is specially designed for a comparison of the patterns of energy deposited by a large range of ions. Low-LET ions are described in a perturbative frame, whereas heavy ions with a very high stopping power are treated using the Lindhard local density approximation and the Russek and Meli statistical method. Ionization cross sections singly differential with energy compare well with the experiment. As an illustration of the non-perturbative interaction of high-LET ions, a comparison between the ion tracks of light and heavy ions with the same specific energy is presented (1.4 MeV/nucleon helium and uranium ions). The mean energy for ejected electrons was found to be approximately four times larger for uranium than for helium, leading to a much larger track radius in the first case. For electrons, except for the excitation cross sections that are deduced from experimental fits, cross sections are derived analytically. For any orientation of the target molecule, the code calculates multiple differential cross sections as a function of the ejection and scattering angles and of the energy transfer. The corresponding singly differential and total ionization cross sections are in good agreement with experimental data. The angular distribution of secondary electrons is shown to depend strongly on the orientation of the water molecule.


Assuntos
Algoritmos , Íons Pesados , Transferência Linear de Energia , Modelos Químicos , Radiometria/métodos , Software , Água/química , Simulação por Computador , Modelos Estatísticos , Método de Monte Carlo , Doses de Radiação
4.
Int J Radiat Biol ; 80(2): 135-45, 2004 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-15164795

RESUMO

PURPOSE: To study the frequency of chromosome aberrations induced by soft X-rays. To see if the core ionization of DNA atoms is involved in this end-point as much as it appears to be in cell killing. MATERIALS AND METHODS: V79 hamster cells were irradiated by synchrotron radiation photons iso-attenuated in the cell (250, 350, 810eV). The morphological chromosome aberrations detected in the first post-irradiation cell division (dicentrics and centric rings) were studied by Giemsa staining. RESULTS: The chromosome aberrations at 350eV were, respectively, 2.6 +/- 0.8 and 2.1 +/- 0.8 times more numerous than at 250 and 810eV for the same average dose absorbed by the nucleus. These relative effectivenesses are comparable with the ones already measured for cell killing. Moreover, they roughly vary such as the relative numbers of core ionizations (including in the phosphorus L-shell) produced in DNA and its bound water (water being involved only at 810eV through the oxygen atoms). In particular, they reproduce the characteristic twofold enhancement at 350eV, above the carbon K threshold. CONCLUSIONS: Correlations suggest that the core ionization process is likely a common and essential mechanism initiating both chromosome aberration and cell killing end-points at these photon energies.


Assuntos
Aberrações Cromossômicas , DNA/efeitos da radiação , Raios X , Animais , Carbono/química , Divisão Celular , Linhagem Celular , Núcleo Celular/metabolismo , Cricetinae , Relação Dose-Resposta à Radiação , Raios gama , Íons , Oxigênio/metabolismo , Fótons , Radiação Ionizante , Radiometria
5.
Radiat Prot Dosimetry ; 99(1-4): 83-4, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12194367

RESUMO

In order to assess the lethal efficiency and other biological effects of inner shell ionisations of constituent atoms of DNA ('K' events), experiments were developed at the LURE synchrotron facility using ultrasoft X rays as a probe of K events. The lethal efficiency of ultrasoft X rays above the carbon K threshold was especially investigated using V79 cells and compared with their efficiency to induce double strand breaks in dry plasmid-DNA. A correlation between the K event efficiencies for these processes is shown. Beams at 340 eV were found to be twice as efficient at killing cells than were beams at 250 eV. In addition, a rough two-fold increase of the relative biological effectiveness for dicentric + ring induction has also been observed between 250 and 340 eV radiations.


Assuntos
DNA/efeitos da radiação , Animais , Linhagem Celular , Cricetinae , Dano ao DNA/efeitos da radiação , Relação Dose-Resposta à Radiação , Potássio , Raios X
6.
Radiat Res ; 157(2): 128-40, 2002 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11835676

RESUMO

The large RBE (approximately 7) measured for the killing of Chinese hamster V79 cells by 340 eV ultrasoft X rays, which preferentially ionize the K shell of carbon atoms (Hervé du Penhoat et al., Radiat. Res. 151, 649-658, 1999), was used to investigate the location of sensitive sites for cell inactivation and the physical modes of action of radiation. The enhancement of the RBE above the carbon K-shell edge either may indicate a high intrinsic efficiency of carbon K-shell ionizations (due, for example, to a specific physical or chemical effect) or may be related to the preferential localization of these ionizations on the DNA. The second interpretation would indicate a strong local (within 3 nm) action of K-shell ionizations and consequently the importance of a direct mechanism for radiation lethality (without excluding an action in conjunction with an indirect component). To distinguish between these two hypotheses, the efficiencies of core ionizations in DNA atoms (phosphorus L-shell, carbon K-shell, and oxygen K-shell ionizations) to induce damages were investigated by measuring their capacities to produce DNA double-strand breaks (DSBs). The effect of photoionizations in isolated DNA was studied using pBS plasmids in a partially hydrated state. No enhancement of the efficiency of DSB induction by carbon K-shell ionizations compared to oxygen K-shell ionizations was found, supporting the hypothesis that it is the localization of these carbon K-shell events on DNA which gives to the 340 eV photons their high killing efficiency. In agreement with this interpretation, cell inactivation and DSB induction, which do not appear to be correlated when expressed in terms of yields per unit dose in the sample, exhibit a rather good correlation when expressed in terms of efficiencies per core event in the DNA. These results suggest that core ionizations in DNA, through core-hole relaxation in conjunction with localized effects of spatially correlated secondary and Auger electrons, may be the major critical events for cell inactivation, and that the resulting DSBs (or a constant fraction of these DSBs) may be a major class of unrepairable lesions.


Assuntos
Dano ao DNA/efeitos da radiação , DNA/efeitos da radiação , Fibroblastos/efeitos da radiação , Raios X/efeitos adversos , Animais , Carbono/efeitos da radiação , Linhagem Celular/efeitos da radiação , Núcleo Celular/efeitos da radiação , Sobrevivência Celular/efeitos da radiação , Cricetinae , Cricetulus , DNA Bacteriano/efeitos da radiação , DNA Recombinante/efeitos da radiação , DNA de Cadeia Simples/efeitos da radiação , DNA Super-Helicoidal/efeitos da radiação , Relação Dose-Resposta à Radiação , Elétrons , Raios gama , Íons , Pulmão/citologia , Modelos Biológicos , Oxigênio/efeitos da radiação , Fósforo/efeitos da radiação , Fótons , Plasmídeos/efeitos da radiação , Eficiência Biológica Relativa
7.
Radiat Res ; 151(6): 649-58, 1999 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10360784

RESUMO

To test a possible specific effect of carbon K-shell ionizations in DNA, survival curves for Chinese hamster V79 cells were measured for X irradiations at energies below and above the carbon K-shell ionization threshold. Specific values of the X-ray energies (250 and 340 eV) were chosen to ensure isoattenuation of the two kinds of radiation within the cell. An enhancement of lethality by a factor of about 2 was found for X rays at 340 eV compared to below the threshold at 250 eV. This may be attributed to the production of highly efficient carbon K-shell ionizations located on DNA. A model of X-ray lethality (Goodhead et al., Radiat. Prot. Dosim. 52, 217-223, 1994) was extended to allow for a possible lethal effect from clusters of reactive species induced by K-shell photoionizations (K-shell clusters). Within this model, the increase in lethality above the carbon K-shell threshold may be explained by a value of 2% for the lethal efficiency of K-shell clusters overlapping the DNA. An extrapolation to the lethal effect of more complex ion-induced K-shell ionizations indicates that K-shell ionization may be a major process in the biological effectiveness of heavy ions.


Assuntos
Núcleo Celular/efeitos da radiação , DNA/efeitos da radiação , Íons Pesados , Animais , Carbono , Linhagem Celular , Sobrevivência Celular/efeitos da radiação , Cricetinae , Cricetulus , Eficiência Biológica Relativa , Raios X
8.
Adv Space Res ; 18(1-2): 15-29, 1996.
Artigo em Inglês | MEDLINE | ID: mdl-11538956

RESUMO

Experimental inactivation cross sections are compared to vacancy production in inner shell (K-shell) of so-called strategic C, N, O atoms of the DNA: in a trial calculation, atoms on the DNA backbone or just contiguous to it are considered as strategic. Various processes are considered: ionization and electron capture by the primary particle, ionization by secondary electrons. The last phenomenon is shown to be important for highly charged and medium velocity ions. A strong similarity is observed between variations versus LET of inactivation and K-vacancy cross sections, especially with regards to LET values for which they maximize: for K-ionization these maxima occur when the ion impact velocity roughly matches the K electron velocity. It is shown that the "K-hypothesis" allows a quantitative fit of experimental inactivation cross sections if a 4% mean lethal efficiency is attributed to ion-induced K-vacancies in strategic C, N, O atoms.


Assuntos
DNA/efeitos da radiação , Íons , Transferência Linear de Energia , Modelos Biológicos , Radiobiologia/métodos , Animais , Bacillus subtilis , Carbono , Morte Celular , Linhagem Celular , Cricetinae , Dano ao DNA , Nitrogênio , Física Nuclear , Oxigênio , Saccharomyces cerevisiae
9.
Int J Radiat Biol ; 65(5): 511-22, 1994 May.
Artigo em Inglês | MEDLINE | ID: mdl-7910190

RESUMO

The existence of a correlation between experimental probabilities of cell inactivation by charged particles and calculated probabilities for K-vacancy production in heavy atoms (C, N, O, P) of the DNA of cell nuclei is established. Both phenomena display a similar dependence upon the linear energy transfer (LET) of incident particles. In particular for low LET values, K cross sections for various incident ions have nearly the same functional dependence on ion-LET and for higher LET-values, K cross sections display maxima which look like those of inactivation cross sections. These characteristics are well-understood features of the K-ionization phenomenon, in particular the maxima of probability occur for projectile velocities near orbital velocities of the ejected electrons. The meaning of the observed correlation is discussed in terms of deposited energy and in the light of existing experimental results on cell inactivation by X-ray absorption at K-threshold. We consider a mechanism which has already been evoked to explain these photo-absorption experiments and which assumes that a K ionization triggers a double-strand break by Coulomb explosion and energy dissipation of Auger electrons. However, it is seen that K cross sections are important for C, N and O atoms but negligible for P atoms. Thus, the lesion considered here affects other atoms than those involved in the K-photoabsorption experiments. The lesion efficiency with respect to subsequent double-strand breakage and repair processes is not yet known, however one may suspect a direct link between DNA blunt ends possibly induced by such K ionizations and cell inactivation.


Assuntos
Morte Celular/efeitos da radiação , DNA/efeitos da radiação , Íons , Animais , Carbono/química , Células Cultivadas , Cricetinae , Cricetulus , DNA/química , Dano ao DNA , Reparo do DNA , Transferência de Energia , Computação Matemática , Oxigênio/química , Tamanho da Partícula
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