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1.
Int J Mol Sci ; 22(19)2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34638573

RESUMO

13-lipoxygenases (13-LOX) catalyze the dioxygenation of various polyunsaturated fatty acids (PUFAs), of which α-linolenic acid (LeA) is converted to 13-S-hydroperoxyoctadeca-9, 11, 15-trienoic acid (13-HPOT), the precursor for the prostaglandin-like plant hormones cis-(+)-12-oxophytodienoic acid (12-OPDA) and methyl jasmonate (MJ). This study aimed for characterizing the four annotated A. thaliana 13-LOX enzymes (LOX2, LOX3, LOX4, and LOX6) focusing on synthesis of 12-OPDA and 4Z,7Z,10Z)-12-[[-(1S,5S)-4-oxo-5-(2Z)-pent-2-en-1yl] cyclopent-2-en-1yl] dodeca-4,7,10-trienoic acid (OCPD). In addition, we performed interaction studies of 13-LOXs with ions and molecules to advance our understanding of 13-LOX. Cell imaging indicated plastid targeting of fluorescent proteins fused to 13-LOXs-N-terminal extensions, supporting the prediction of 13-LOX localization to plastids. The apparent maximal velocity (Vmax app) values for LOX-catalyzed LeA oxidation were highest for LOX4 (128 nmol·s-1·mg protein-1), with a Km value of 5.8 µM. A. thaliana 13-LOXs, in cascade with 12-OPDA pathway enzymes, synthesized 12-OPDA and OCPD from LeA and docosahexaenoic acid, previously shown only for LOX6. The activities of the four isoforms were differently affected by physiologically relevant chemicals, such as Mg2+, Ca2+, Cu2+ and Cd2+, and by 12-OPDA and MJ. As demonstrated for LOX4, 12-OPDA inhibited enzymatic LeA hydroperoxidation, with half-maximal enzyme inhibition at 48 µM. Biochemical interactions, such as the sensitivity of LOX toward thiol-reactive agents belonging to cyclopentenone prostaglandins, are suggested to occur in human LOX homologs. Furthermore, we conclude that 13-LOXs are isoforms with rather specific functional and regulatory enzymatic features.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Lipoxigenase/metabolismo , Acetatos/metabolismo , Sequência de Aminoácidos , Ciclopentanos/metabolismo , Ácidos Graxos Insaturados/metabolismo , Regulação da Expressão Gênica de Plantas/fisiologia , Ácidos Linoleicos/metabolismo , Oxilipinas/metabolismo
2.
FEBS Lett ; 595(21): 2715-2730, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34561866

RESUMO

Thioredoxins (TRXs), a large subclass of ubiquitous oxidoreductases, are involved in thiol redox regulation. Here, we performed a comprehensive analysis of TRXs in the Arabidopsis thaliana genome, revealing 41 genes encoding 18 typical and 23 atypical TRXs, and 6 genes encoding thioredoxin reductases (TRs). The high number of atypical TRXs indicates special functions in plants that mostly await elucidation. We identified an atypical class of thioredoxins called TRX-c in the genomes of photosynthetic eukaryotes. Localized to the chloroplast, TRX-c displays atypical CPLC, CHLC and CNLC motifs in the active sites. In silico analysis of the transcriptional regulations of TRXs revealed high expression of TRX-c in leaves and strong regulation under cold, osmotic, salinity and metal ion stresses.


Assuntos
Arabidopsis , Tiorredoxinas , Cloroplastos , Regulação da Expressão Gênica de Plantas , Fotossíntese
3.
Biomolecules ; 11(3)2021 03 18.
Artigo em Inglês | MEDLINE | ID: mdl-33803875

RESUMO

α,ß-unsaturated carbonyls interfere with numerous plant physiological processes. One mechanism of action is their reactivity toward thiols of metabolites like cysteine and glutathione (GSH). This work aimed at better understanding these interactions. Both 12-oxophytodienoic acid (12-OPDA) and abscisic acid (ABA) conjugated with cysteine. It was found that the reactivity of α,ß-unsaturated carbonyls with GSH followed the sequence trans-2-hexenal < 12-OPDA ≈ 12-OPDA-ethylester < 2-cyclopentenone << methyl vinylketone (MVK). Interestingly, GSH, but not ascorbate (vitamin C), supplementation ameliorated the phytotoxic potential of MVK. In addition, 12-OPDA and 12-OPDA-related conjugated carbonyl compounds interacted with proteins, e.g., with members of the thioredoxin (TRX)-fold family. 12-OPDA modified two cysteinyl residues of chloroplast TRX-f1. The OPDAylated TRX-f1 lost its activity to activate the Calvin-Benson-cycle enzyme fructose-1,6-bisphosphatase (FBPase). Finally, we show that 12-OPDA interacts with cyclophilin 20-3 (Cyp20-3) non-covalently and affects its peptidyl-prolyl-cis/trans isomerase activity. The results demonstrate the high potential of 12-OPDA as a diverse interactor and cellular regulator and suggest that OPDAylation may occur in plant cells and should be investigated as novel regulatory mechanism.


Assuntos
Antioxidantes/química , Ácidos Graxos Insaturados/química , Reguladores de Crescimento de Plantas/química , Compostos de Sulfidrila/química , Arabidopsis/química , Cisteína/química , Tiorredoxinas/química
4.
Biomolecules ; 10(8)2020 07 30.
Artigo em Inglês | MEDLINE | ID: mdl-32751472

RESUMO

ß-carbonic anhydrases (ßCA) accelerate the equilibrium formation between CO2 and carbonate. Two plant ßCA isoforms are targeted to the chloroplast and represent abundant proteins in the range of >1% of chloroplast protein. While their function in gas exchange and photosynthesis is well-characterized in carbon concentrating mechanisms of cyanobacteria and plants with C4-photosynthesis, their function in plants with C3-photosynthesis is less clear. The presence of conserved and surface-exposed cysteinyl residues in the ßCA-structure urged to the question whether ßCA is subject to redox regulation. Activity measurements revealed reductive activation of ßCA1, whereas oxidized ßCA1 was inactive. Mutation of cysteinyl residues decreased ßCA1 activity, in particular C280S, C167S, C230S, and C257S. High concentrations of dithiothreitol or low amounts of reduced thioredoxins (TRXs) activated oxidized ßCA1. TRX-y1 and TRX-y2 most efficiently activated ßCA1, followed by TRX-f1 and f2 and NADPH-dependent TRX reductase C (NTRC). High light irradiation did not enhance ßCA activity in wildtype Arabidopsis, but surprisingly in ßca1 knockout plants, indicating light-dependent regulation. The results assign a role of ßCA within the thiol redox regulatory network of the chloroplast.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Anidrases Carbônicas/metabolismo , Modelos Moleculares , Oxirredução , Compostos de Sulfidrila/metabolismo , Tiorredoxinas/metabolismo
5.
PLoS Comput Biol ; 16(1): e1007102, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31951606

RESUMO

Cells contain a thiol redox regulatory network to coordinate metabolic and developmental activities with exogenous and endogenous cues. This network controls the redox state and activity of many target proteins. Electrons are fed into the network from metabolism and reach the target proteins via redox transmitters such as thioredoxin (TRX) and NADPH-dependent thioredoxin reductases (NTR). Electrons are drained from the network by reactive oxygen species (ROS) through thiol peroxidases, e.g., peroxiredoxins (PRX). Mathematical modeling promises access to quantitative understanding of the network function and was implemented by using published kinetic parameters combined with fitting to known biochemical data. Two networks were assembled, namely the ferredoxin (FDX), FDX-dependent TRX reductase (FTR), TRX, fructose-1,6-bisphosphatase (FBPase) pathway with 2-cysteine PRX/ROS as oxidant, and separately the FDX, FDX-dependent NADP reductase (FNR), NADPH, NTRC-pathway for 2-CysPRX reduction. Combining both modules allowed drawing several important conclusions of network performance. The resting H2O2 concentration was estimated to be about 30 nM in the chloroplast stroma. The electron flow to metabolism exceeds that into thiol regulation of FBPase more than 7000-fold under physiological conditions. The electron flow from NTRC to 2-CysPRX is about 5.32-times more efficient than that from TRX-f1 to 2-CysPRX. Under severe stress (30 µM H2O2) the ratio of electron flow to the thiol network relative to metabolism sinks to 1:251 whereas the ratio of e- flow from NTRC to 2-CysPRX and TRX-f1 to 2-CysPRX rises up to 1:67. Thus, the simulation provides clues on experimentally inaccessible parameters and describes the functional state of the chloroplast thiol regulatory network.


Assuntos
Cloroplastos/metabolismo , Redes e Vias Metabólicas/fisiologia , Oxirredução , Espécies Reativas de Oxigênio/metabolismo , Arabidopsis/metabolismo , Arabidopsis/fisiologia , Proteínas de Arabidopsis/metabolismo , Simulação por Computador , Ferredoxinas/metabolismo , Peróxido de Hidrogênio/metabolismo , Proteínas Ferro-Enxofre/metabolismo , NADP/metabolismo , Oxirredutases/metabolismo , Compostos de Sulfidrila/metabolismo , Tiorredoxinas/metabolismo
6.
Antioxidants (Basel) ; 8(4)2019 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-30965652

RESUMO

Water deficiency compromises plant performance and yield in many habitats and in agriculture. In addition to survival of the acute drought stress period which depends on plant-genotype-specific characteristics, stress intensity and duration, also the speed and efficiency of recovery determine plant performance. Drought-induced deregulation of metabolism enhances generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) which in turn affect the redox regulatory state of the cell. Strong correlative and analytical evidence assigns a major role in drought tolerance to the redox regulatory and antioxidant system. This review compiles current knowledge on the response and function of superoxide, hydrogen peroxide and nitric oxide under drought stress in various species and drought stress regimes. The meta-analysis of reported changes in transcript and protein amounts, and activities of components of the antioxidant and redox network support the tentative conclusion that drought tolerance is more tightly linked to up-regulated ascorbate-dependent antioxidant activity than to the response of the thiol-redox regulatory network. The significance of the antioxidant system in surviving severe phases of dehydration is further supported by the strong antioxidant system usually encountered in resurrection plants.

7.
Elife ; 72018 10 12.
Artigo em Inglês | MEDLINE | ID: mdl-30311601

RESUMO

Thiol-dependent redox regulation controls central processes in plant cells including photosynthesis. Thioredoxins reductively activate, for example, Calvin-Benson cycle enzymes. However, the mechanism of oxidative inactivation is unknown despite its importance for efficient regulation. Here, the abundant 2-cysteine peroxiredoxin (2-CysPrx), but not its site-directed variants, mediates rapid inactivation of reductively activated fructose-1,6-bisphosphatase and NADPH-dependent malate dehydrogenase (MDH) in the presence of the proper thioredoxins. Deactivation of phosphoribulokinase (PRK) and MDH was compromised in 2cysprxAB mutant plants upon light/dark transition compared to wildtype. The decisive role of 2-CysPrx in regulating photosynthesis was evident from reoxidation kinetics of ferredoxin upon darkening of intact leaves since its half time decreased 3.5-times in 2cysprxAB. The disadvantage of inefficient deactivation turned into an advantage in fluctuating light. Physiological parameters like MDH and PRK inactivation, photosynthetic kinetics and response to fluctuating light fully recovered in 2cysprxAB mutants complemented with 2-CysPrxA underlining the significance of 2-CysPrx. The results show that the 2-CysPrx serves as electron sink in the thiol network important to oxidize reductively activated proteins and represents the missing link in the reversal of thioredoxin-dependent regulation.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Cloroplastos/metabolismo , Cisteína/metabolismo , Peroxirredoxinas/metabolismo , Tiorredoxinas/metabolismo , Arabidopsis/efeitos da radiação , Clorofila A/metabolismo , Simulação por Computador , Fluorescência , Genótipo , Meia-Vida , Cinética , Luz , Malato Desidrogenase/metabolismo , Modelos Biológicos , Mutação/genética , NADP/metabolismo , Oxirredução , Fenótipo , Fotossíntese , Espectroscopia de Luz Próxima ao Infravermelho
8.
FEBS Lett ; 592(6): 1030-1041, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29453875

RESUMO

Plastidial thioredoxin (TRX)-like2.1 proteins are atypical thioredoxins possessing a WCRKC active site signature and using glutathione for recycling. To obtain structural information supporting the peculiar catalytic mechanisms and target proteins of these TRXs, we solved the crystal structures of poplar TRX-like2.1 in oxidized and reduced states and of mutated variants. These structures share similar folding with TRXs exhibiting the canonical WCGPC signature. Moreover, the overall conformation is not altered by reduction of the catalytic disulfide bond or in a C45S/C67S variant that formed a disulfide-bridged dimer possibly mimicking reaction intermediates with target proteins. Modeling of the interaction of TRX-like2.1 with both NADPH- and ferredoxin-thioredoxin reductases (FTR) indicates that the presence of Arg43 and Lys44 residues likely precludes reduction by the plastidial FTR.


Assuntos
Proteínas de Cloroplastos/química , Mutação , Populus/enzimologia , Tiorredoxinas/química , Catálise , Domínio Catalítico , Proteínas de Cloroplastos/genética , Cristalografia por Raios X , Oxirredução , Populus/genética , Tiorredoxinas/genética
9.
Plant Sci ; 252: 257-266, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27717462

RESUMO

A cDNA coding for a plastidic P2-type G6PDH isoform from poplar (Populus tremula x tremuloides) has been used to express and purify to homogeneity the mature recombinant protein with a N-terminus His-tag. The study of the kinetic properties of the recombinant enzyme showed an in vitro redox sensing modulation exerted by reduced DTT. The interaction with thioredoxins (TRXs) was then investigated. Five cysteine to serine variants (C145S - C175S - C183S - C195S - C242S) and a variant with a double substitution for Cys175 and Cys183 (C175S/C183S) have been generated, purified and biochemically characterized in order to investigate the specific role(s) of cysteines in terms of redox regulation and NADPH-dependent inhibition. Three cysteine residues (C145, C194, C242) are suggested to have a role in controlling the NADP+ access to the active site, and in stabilizing the NADPH regulatory binding site. Our results also indicate that the regulatory disulfide involves residues Cys175 and Cys183 in a position similar to those of chloroplastic P1-G6PDHs, but the modulation is exerted primarily by TRX m-type, in contrast to P1-G6PDH, which is regulated by TRX f. This unexpected specificity indicates differences in the mechanism of regulation, and redox sensing of plastidic P2-G6PDH compared to chloroplastic P1-G6PDH in higher plants.


Assuntos
Glucosefosfato Desidrogenase/fisiologia , Proteínas de Plantas/fisiologia , Plastídeos/metabolismo , Populus/metabolismo , Tiorredoxinas/fisiologia , Motivos de Aminoácidos , Sequência de Aminoácidos , Sítios de Ligação , Clonagem Molecular , Cisteína/química , Cisteína/fisiologia , Glucosefosfato Desidrogenase/química , Glucosefosfato Desidrogenase/metabolismo , Mutagênese Sítio-Dirigida , NADP/antagonistas & inibidores , NADP/química , Oxirredução , Via de Pentose Fosfato , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Populus/genética , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência , Tiorredoxinas/química , Tiorredoxinas/metabolismo
10.
Appl Environ Microbiol ; 80(20): 6316-27, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25107961

RESUMO

The first steps of wood degradation by fungi lead to the release of toxic compounds known as extractives. To better understand how lignolytic fungi cope with the toxicity of these molecules, a transcriptomic analysis of Phanerochaete chrysosporium genes was performed in the presence of oak acetonic extracts. It reveals that in complement to the extracellular machinery of degradation, intracellular antioxidant and detoxification systems contribute to the lignolytic capabilities of fungi, presumably by preventing cellular damages and maintaining fungal health. Focusing on these systems, a glutathione transferase (P. chrysosporium GTT2.1 [PcGTT2.1]) has been selected for functional characterization. This enzyme, not characterized so far in basidiomycetes, has been classified first as a GTT2 compared to the Saccharomyces cerevisiae isoform. However, a deeper analysis shows that the GTT2.1 isoform has evolved functionally to reduce lipid peroxidation by recognizing high-molecular-weight peroxides as substrates. Moreover, the GTT2.1 gene has been lost in some non-wood-decay fungi. This example suggests that the intracellular detoxification system evolved concomitantly with the extracellular ligninolytic machinery in relation to the capacity of fungi to degrade wood.


Assuntos
Glutationa Transferase/metabolismo , Phanerochaete/efeitos dos fármacos , Phanerochaete/genética , Extratos Vegetais/farmacologia , Quercus/química , Acetona/química , Evolução Molecular , Regulação Fúngica da Expressão Gênica , Glutationa Transferase/genética , Inativação Metabólica , Isoenzimas , Lignina/metabolismo , Peroxidação de Lipídeos , Estresse Oxidativo/efeitos dos fármacos , Peróxidos/química , Peróxidos/metabolismo , Phanerochaete/metabolismo , Extratos Vegetais/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Madeira/microbiologia
11.
Plant Physiol Biochem ; 73: 266-73, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24161756

RESUMO

In plant cells, the plastidial glucose 6-phosphate dehydrogenase (P2-G6PDH, EC 1.1.1.49) represents one of the most important sources of NADPH. However, previous studies revealed that both native and recombinant purified P2-G6PDHs show a great instability and a rapid loss of catalytic activity. Therefore it has been difficult to describe accurately the catalytic and physico-chemical properties of these isoforms. The plastidial G6PDH encoding sequence from barley roots (Hordeum vulgare cv. Nure), devoid of a long plastidial transit peptide, was expressed as recombinant protein in Escherichia coli, either untagged or with an N-terminal his-tag. After purification from both the soluble fraction and inclusion bodies, we have explored its kinetic parameters, as well as its sensitivity to reduction. The obtained results are consistent with values determined for other P2-G6PDHs previously purified from barley roots and from other land plants. Overall, these data shed light on the catalytic mechanism of plant P2-G6PDH, summarized in a proposed model in which the sequential mechanism is very similar to the mammalian cytosolic G6PDH. This study provides a rational basis to consider the recombinant barley root P2-G6PDH as a good model for further kinetic and structural studies.


Assuntos
Genes de Plantas , Glucosefosfato Desidrogenase/genética , Hordeum/genética , NADP/genética , Proteínas de Plantas/genética , Raízes de Plantas/metabolismo , Plastídeos/genética , Sequência de Aminoácidos , Animais , Escherichia coli , Glucosefosfato Desidrogenase/metabolismo , Hordeum/enzimologia , Hordeum/metabolismo , Mamíferos , Dados de Sequência Molecular , NADP/metabolismo , Proteínas de Plantas/metabolismo , Plastídeos/metabolismo , Isoformas de Proteínas , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo
12.
Front Plant Sci ; 4: 105, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23641245

RESUMO

Living organisms are subjected to oxidative stress conditions which are characterized by the production of reactive oxygen, nitrogen, and sulfur species. In plants as in other organisms, many of these compounds have a dual function as they damage different types of macromolecules but they also likely fulfil an important role as secondary messengers. Owing to the reactivity of their thiol groups, some protein cysteine residues are particularly prone to oxidation by these molecules. In the past years, besides their recognized catalytic and regulatory functions, the modification of cysteine thiol group was increasingly viewed as either protective or redox signaling mechanisms. The most physiologically relevant reversible redox post-translational modifications (PTMs) are disulfide bonds, sulfenic acids, S-glutathione adducts, S-nitrosothiols and to a lesser extent S-sulfenyl-amides, thiosulfinates and S-persulfides. These redox PTMs are mostly controlled by two oxidoreductase families, thioredoxins and glutaredoxins. This review focuses on recent advances highlighting the variety and physiological roles of these PTMs and the proteomic strategies used for their detection.

13.
Microb Biotechnol ; 6(3): 248-63, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23279857

RESUMO

Fungal degradation of wood is mainly restricted to basidiomycetes, these organisms having developed complex oxidative and hydrolytic enzymatic systems. Besides these systems, wood-decaying fungi possess intracellular networks allowing them to deal with the myriad of potential toxic compounds resulting at least in part from wood degradation but also more generally from recalcitrant organic matter degradation. The members of the detoxification pathways constitute the xenome. Generally, they belong to multigenic families such as the cytochrome P450 monooxygenases and the glutathione transferases. Taking advantage of the recent release of numerous genomes of basidiomycetes, we show here that these multigenic families are extended and functionally related in wood-decaying fungi. Furthermore, we postulate that these rapidly evolving multigenic families could reflect the adaptation of these fungi to the diversity of their substrate and provide keys to understand their ecology. This is of particular importance for white biotechnology, this xenome being a putative target for improving degradation properties of these fungi in biomass valorization purposes.


Assuntos
Adaptação Fisiológica/genética , Basidiomycota/enzimologia , Sistema Enzimático do Citocromo P-450/metabolismo , Glutationa Transferase/metabolismo , Redes e Vias Metabólicas/genética , Madeira/química , Madeira/microbiologia , Basidiomycota/genética , Basidiomycota/metabolismo , Basidiomycota/fisiologia , Biodegradação Ambiental , Sistema Enzimático do Citocromo P-450/genética , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Genoma Fúngico , Glutationa Transferase/genética , Madeira/metabolismo , Xenobióticos/metabolismo
14.
Environ Microbiol ; 15(3): 795-810, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22891731

RESUMO

Legumes interact symbiotically with bacteria of the Rhizobiaceae to form nitrogen-fixing root nodules. We investigated the contribution of the three glutaredoxin (Grx)-encoding genes present in the Sinorhizobium meliloti genome to this symbiosis. SmGRX1 (CGYC active site) and SmGRX3 (CPYG) recombinant proteins displayed deglutathionylation activity in the 2-hydroethyldisulfide assay, whereas SmGRX2 (CGFS) did not. Mutation of SmGRX3 did not affect S. meliloti growth or symbiotic capacities. In contrast, SmGRX1 and SmGRX2 mutations decreased the growth of free-living bacteria and the nitrogen fixation capacity of bacteroids. Mutation of SmGRX1 led to nodule abortion and an absence of bacteroid differentiation, whereas SmGRX2 mutation decreased nodule development without modifying bacteroid development. The higher sensitivity of the Smgrx1 mutant strain as compared with wild-type strain to oxidative stress was associated with larger amounts of glutathionylated proteins. The Smgrx2 mutant strain displayed significantly lower levels of activity than the wild type for two iron-sulfur-containing enzymes, aconitase and succinate dehydrogenase. This lower level of activity could be associated with deregulation of the transcriptional activity of the RirA iron regulator and higher intracellular iron content. Thus, two S. meliloti Grx proteins are essential for symbiotic nitrogen fixation, playing independent roles in bacterial differentiation and the regulation of iron metabolism.


Assuntos
Glutarredoxinas/genética , Glutarredoxinas/metabolismo , Ferro/metabolismo , Sinorhizobium meliloti/genética , Sinorhizobium meliloti/metabolismo , Simbiose , Fabaceae/microbiologia , Perfilação da Expressão Gênica , Regulação Bacteriana da Expressão Gênica , Mutação , Fixação de Nitrogênio/genética , Filogenia , Nódulos Radiculares de Plantas/citologia , Nódulos Radiculares de Plantas/crescimento & desenvolvimento , Nódulos Radiculares de Plantas/microbiologia , Sinorhizobium meliloti/classificação , Sinorhizobium meliloti/crescimento & desenvolvimento , Succinato Desidrogenase/metabolismo
15.
J Proteome Res ; 11(11): 5418-32, 2012 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-22985405

RESUMO

Despite having very similar initial pools of stored mRNAs and proteins in the dry state, mature Arabidopsis seeds can either proceed toward radicle protrusion or stay in a dormant state upon imbibition. Dormancy breaking, a prerequisite to germination completion, can be induced by different treatments though the underlying mechanisms remain elusive. Thus, we investigated the consequence of such treatments on the seed proteome. Two unrelated dormancy-releasing treatments were applied to dormant seeds, namely, cold stratification and exogenous nitrates, in combination with differential proteomic tools to highlight the specificities of the imbibed dormant state. The results reveal that both treatments lead to highly similar proteome adjustments. In the imbibed dormant state, enzymes involved in reserve mobilization are less accumulated and it appears that several energetically costly processes associated to seed germination and preparation for subsequent seedling establishment are repressed. Our data suggest that dormancy maintenance is associated to an abscisic-acid-dependent recapitulation of the late maturation program resulting in a higher potential to cope with environmental stresses. The comparison of the present results with previously published -omic data sets reinforces and extends the assumption that post-transcriptional, translational, and post-translational regulations are determinant for seed germination.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/embriologia , Temperatura Baixa , Nitratos/metabolismo , Proteoma , Sementes/metabolismo , Arabidopsis/metabolismo , Eletroforese em Gel Bidimensional , Germinação , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Transcriptoma
16.
Plant Physiol ; 159(2): 592-605, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22523226

RESUMO

Plant thioredoxins (Trxs) constitute a complex family of thiol oxidoreductases generally sharing a WCGPC active site sequence. Some recently identified plant Trxs (Clot, Trx-like1 and -2, Trx-lilium1, -2, and -3) display atypical active site sequences with altered residues between the two conserved cysteines. The transcript expression patterns, subcellular localizations, and biochemical properties of some representative poplar (Populus spp.) isoforms were investigated. Measurements of transcript levels for the 10 members in poplar organs indicate that most genes are constitutively expressed. Using transient expression of green fluorescent protein fusions, Clot and Trx-like1 were found to be mainly cytosolic, whereas Trx-like2.1 was located in plastids. All soluble recombinant proteins, except Clot, exhibited insulin reductase activity, although with variable efficiencies. Whereas Trx-like2.1 and Trx-lilium2.2 were efficiently regenerated both by NADPH-Trx reductase and glutathione, none of the proteins were reduced by the ferredoxin-Trx reductase. Only Trx-like2.1 supports the activity of plastidial thiol peroxidases and methionine sulfoxide reductases employing a single cysteine residue for catalysis and using a glutathione recycling system. The second active site cysteine of Trx-like2.1 is dispensable for this reaction, indicating that the protein possesses a glutaredoxin-like activity. Interestingly, the Trx-like2.1 active site replacement, from WCRKC to WCGPC, suppresses its capacity to use glutathione as a reductant but is sufficient to allow the regeneration of target proteins employing two cysteines for catalysis, indicating that the nature of the residues composing the active site sequence is crucial for substrate selectivity/recognition. This study provides another example of the cross talk existing between the glutathione/glutaredoxin and Trx-dependent pathways.


Assuntos
Cisteína/metabolismo , Populus/enzimologia , Tiorredoxinas/metabolismo , Sequência de Aminoácidos , Sequência de Bases , Domínio Catalítico , Proteínas de Cloroplastos/genética , Proteínas de Cloroplastos/metabolismo , Cisteína/genética , Citosol/metabolismo , Ácido Ditionitrobenzoico/química , Ativação Enzimática , Perfilação da Expressão Gênica , Regulação Enzimológica da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Glutarredoxinas/química , Glutarredoxinas/genética , Glutationa/metabolismo , Proteínas Ferro-Enxofre/genética , Proteínas Ferro-Enxofre/metabolismo , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , NADP/química , Oxirredução , Oxirredutases/genética , Oxirredutases/metabolismo , Células Vegetais/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plastídeos/genética , Plastídeos/metabolismo , Populus/genética , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Solubilidade , Espectrometria de Massas por Ionização por Electrospray , Especificidade por Substrato , Tiorredoxina Dissulfeto Redutase/genética , Tiorredoxina Dissulfeto Redutase/metabolismo , Tiorredoxinas/genética
17.
Methods Mol Biol ; 773: 215-36, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21898259

RESUMO

The seed is the dispersal unit of plants and must survive the vagaries of the environment. It is the object of intense genetic and genomic studies because processes related to seed quality affect crop yield and the seed itself provides food for humans and animals. Presently, the general aim of postgenomics analyses is to understand the complex biochemical and molecular processes underlying seed quality, longevity, dormancy, and vigor. Due to advances in functional genomics, the recent past years have seen a tremendous progress in our understanding of several aspects of seed development and germination. Here, we describe the proteomics protocols (from protein extraction to mass spectrometry) that can be used to investigate several aspects of seed physiology, including germination and its hormonal regulation, dormancy release, and seed longevity. These techniques can be applied to the study of both model plants (such as Arabidopsis) and crops.


Assuntos
Arabidopsis/crescimento & desenvolvimento , Germinação/genética , Dormência de Plantas/genética , Proteômica , Sementes/crescimento & desenvolvimento , Ácido Abscísico/genética , Ácido Abscísico/metabolismo , Ácido Abscísico/fisiologia , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Regulação da Expressão Gênica de Plantas , Germinação/fisiologia , Espectrometria de Massas , Dormência de Plantas/fisiologia , Reguladores de Crescimento de Plantas/genética , Reguladores de Crescimento de Plantas/metabolismo , Processamento de Proteína Pós-Traducional , Sementes/genética
18.
J Exp Bot ; 62(11): 4013-23, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21464159

RESUMO

Total glucose-6-phosphate dehydrogenase (G6PDH) activity, protein abundance, and transcript levels of G6PDH isoforms were measured in response to exogenous abscisic acid (ABA) supply to barley (Hordeum vulgare cv Nure) hydroponic culture. Total G6PDH activity increased by 50% in roots treated for 12 h with exogenous 0.1 mM ABA. In roots, a considerable increase (35%) in plastidial P2-G6PDH transcript levels was observed during the first 3 h of ABA treatment. Similar protein variations were observed in immunoblotting analyses. In leaves, a 2-fold increase in total G6PDH activity was observed after ABA treatment, probably related to an increase in the mRNA level (increased by 50%) and amount of protein (increased by 85%) of P2-G6PDH. Together these results suggest that the plastidial P2-isoform plays an important role in ABA-treated barley plants.


Assuntos
Ácido Abscísico/farmacologia , Glucosefosfato Desidrogenase/genética , Glucosefosfato Desidrogenase/metabolismo , Hordeum/genética , Hordeum/metabolismo , Regulação da Expressão Gênica de Plantas , Hidroponia , Filogenia , Folhas de Planta/genética , Folhas de Planta/metabolismo , Raízes de Plantas/genética , Raízes de Plantas/metabolismo , Plastídeos/enzimologia , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Análise de Sequência de Proteína
19.
FEBS Lett ; 585(7): 1077-81, 2011 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-21385584

RESUMO

Trx-z is a chloroplastic thioredoxin, exhibiting a usual WCGPC active site, but whose biochemical properties are unknown. We demonstrate here that Trx-z supports the activity of several plastidial antioxidant enzymes, such as thiol-peroxidases and methionine sulfoxide reductases, using electrons provided by ferredoxin-thioredoxin reductase. Its disulfide reductase activity requires the presence of both active site cysteines forming a catalytic disulfide bridge with a midpoint redox potential of -251 mV at pH7. These in vitro biochemical data suggest that, besides its decisive role in the regulation of plastidial transcription, Trx-z might also be involved in stress response.


Assuntos
Antioxidantes/metabolismo , Proteínas de Plantas/metabolismo , Populus/metabolismo , Tiorredoxinas/metabolismo , Transporte de Elétrons , Proteínas Ferro-Enxofre/metabolismo , Oxirredutases/metabolismo , Plastídeos/metabolismo , Populus/citologia , Populus/enzimologia , Tiorredoxina Dissulfeto Redutase/metabolismo
20.
Photosynth Res ; 104(1): 75-99, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19902380

RESUMO

The post-translational modification consisting in the formation/reduction of disulfide bonds has been the subject of intense research in plants since the discovery in the 1970s that many chloroplastic enzymes are regulated by light through dithiol-disulfide exchange reactions catalyzed by oxidoreductases called thioredoxins (Trxs). Further biochemical and proteomic studies have considerably increased the number of target enzymes and processes regulated by these mechanisms in many sub-cellular compartments. Recently, glutathionylation, a modification consisting in the reversible formation of a glutathione adduct on cysteine residues, was proposed as an alternative redox regulation mechanism. Glutaredoxins (Grxs), proteins related to Trxs, are efficient catalysts for deglutathionylation, the opposite reaction. Hence, the Trxs- and Grxs-dependent pathways might constitute complementary and not only redundant regulatory processes. This article focuses on these two multigenic families and associated protein partners in poplar and on their involvement in the regulation of some major chloroplastic processes such as stress response, carbohydrate and heme/chlorophyll metabolism.


Assuntos
Antioxidantes/fisiologia , Cloroplastos/metabolismo , Populus/metabolismo , Antioxidantes/metabolismo , Metabolismo dos Carboidratos/fisiologia , Cloroplastos/enzimologia , Cloroplastos/fisiologia , Ferredoxinas/metabolismo , Genes de Plantas/genética , Genes de Plantas/fisiologia , Glutarredoxinas/metabolismo , Glutarredoxinas/fisiologia , Fotossíntese/fisiologia , Populus/enzimologia , Populus/genética , Populus/fisiologia , Proteômica , Estresse Fisiológico/fisiologia , Tiorredoxinas/metabolismo
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