Innovative hyper-thermophilic aerobic submerged membrane distillation bioreactor for wastewater reclamation.

For the first time, a hyper-thermophilic aerobic (>60 °C) bioreactor has been integrated with direct submerged membrane distillation (MD), highlighting its potential as an advanced wastewater treatment solution. The hyper-thermophilic aerobic bioreactor, operating up to 65 °C, is tailored for high organic removal, while MD efficiently produces clean water. Throughout the study, high removal rates of 99.5% for organic matter, 96.4% for ammonia, and 100% for phosphorus underscored the impressive adaptability of microorganisms to challenging hyper-thermophilic conditions and a successful combination with the MD process. Despite the extreme temperatures and substantial salinity accumulation reaching up to 12,532 µS/cm, the biomass of microorganisms increased by 1.6 times over a 92-day period, representing their remarkable resilience. The distillation flux ranged from 6.15 LMH to 8.25 LMH, benefiting from the temperature gradient in the hyper-thermophilic setting and the design of the tubular submerged MD membrane module. The system also excels in pH control, utilizing fewer alkali and nutritional resources than conventional systems. Meiothermus, Firmicutes, and Bacteroidetes, the three dominant species, played a crucial role, showcasing their significance in adapting to high salinity and decomposing organic matter.

Water reclamation and microbial community investigation: Treatment of tetramethylammonium hydroxide wastewater through an anaerobic osmotic membrane bioreactor hybrid system.

Tetramethylammonium hydroxide (TMAH) is a toxic photoresist developer used in the photolithography process in thin-film transistor liquid crystal display (TFT-LCD) production, and it can be removed through anaerobic treatment. TMAH cannot be released into the environment because of its higher toxicity. A tight membrane, such as a forward osmosis (FO) membrane, together with an anaerobic biological process can ensure that no TMAH is released into the environment. Thus, for the first time, an anaerobic osmotic membrane bioreactor (AnOMBR) hybrid system was developed in this study to treat a low-strength TMAH wastewater and to simultaneously investigate its microbial community. Microfiltration extraction was used to mitigate the salinity accumulation, and a periodically physical water cleaning was utilized to mitigate the FO membrane fouling. The diluted draw solute (MgSO4) was reconcentrated and reused by a membrane distillation (MD) process in the AnOMBR to achieve 99.99% TMAH removal in this AnOMBR-MD hybrid system, thereby ensuring that no TMAH is released into the natural environment. Moreover, the membrane fouling in the feed and draw sides were analyzed through the fluorescence excitation-emission matrix (FEEM) spectrophotometry to confirm that the humic acid-like materials were the primary membrane fouling components in this AnOMBR. Additionally, 16S rRNA metagenomics analysis indicated that Methanosaeta was the predominant contributor to methanogenesis and proliferated during the long-term operation. The methane yield was increased from 0.2 to 0.26 L CH4/g COD when the methanogen species acclimatized to the saline system.

A novel thermophilic anaerobic granular sludge membrane distillation bioreactor for wastewater reclamation.

Membrane distillation (MD) has a high heat requirement. Integrating MD with thermophilic bioreactors could remedy this problem. A laboratory-scale thermophilic anaerobic granular sludge membrane distillation bioreactor (ThAGS-MDBR) was used to treat wastewater with a high organic loading rate (OLR). Waste heat from ThAGS was used directly for the MD process to reduce energy consumption. The result demonstrated that the ThAGS-MDBR system achieved a high-efficiency removal of chemical oxygen demand (more 99.5%) and NH4+-N (96.4%). Furthermore, the highest methane production from the proposed system was 332 mL/g CODremoved at OLR of 16 kg COD/m3/day. Specifically, an aggregate of densely packed diverse microbial communities in anaerobic granular sludge was the main mechanism for the enhancement of bioreactor tolerance with environmental changes. High-quality distillate water from ThAGS-MDBR was reclaimed in one step with total organic carbon less than 1.7 mg/L and electrical conductivity less than 120 µS/cm. Furthermore, the result of the DNA extraction kit recorded that Methanosaeta thermophila was a critical archaea for high COD removal and bioreactor stability.

Innovative upflow anaerobic sludge osmotic membrane bioreactor for wastewater treatment.

A novel upflow anaerobic sludge-forward osmotic membrane bioreactor was developed for simultaneous wastewater treatment, membrane fouling reduction, and nutrient recovery. An upflow anaerobic sludge blanket (UASB) reactor was incorporated into the system, suspending the anaerobic sludge at the bottom of the reactor. A forward osmosis membrane replaced the traditional three-phase separator of the UASB technology. The removals of chemical oxygen demand, PO43-, and NH4+ were all more than 95% with low membrane fouling in this system. Halotolerant Fusibacter, which can ferment organics to acetate, was increased rapidly from 0.1% to 5% in this saline environment. Acetoclastic Methanosaeta was the most dominant prokaryotes and responsible for majority of methane production. Reduction of membrane fouling in this system was verified by the fluorescence excitation-emission matrix spectrophotometry. Furthermore, phosphorus recovery and salinity build-up mitigation were achieved using periodic microfiltration to recover 57-105 mg/L phosphorus from pH 9 to 12.

Comparing Ozonation and Biofiltration Treatment of Source Water with High Cyanobacteria-Derived Organic Matter: The Case of a Water Treatment Plant Followed by a Small-Scale Water Distribution System.

High cyanobacteria-derived dissolved organic carbon (DOC) in source water can cause drinking water quality to deteriorate, producing bad taste, odor, toxins, and possibly elevated levels of disinfection byproduct (DBP) precursors. Conventional water treatment processes do not effectively remove algal organic substances. In this study, rapid-sand-filtration effluent from a water treatment plant on Kinmen Island, where serious cyanobacterial blooms occurred, was used to evaluate the DOC- and DBP-removal efficiency of ozonation and/or biofiltration. To simulate a small-scale water distribution system following water treatment, 24 h simulated distribution system (SDS) tests were conducted. The following DBPs were analyzed: trihalomethanes (THMs), haloacetic acids (HAAs), haloacetonitriles (HANs), and trichloronitromethane (TCNM). Applying biological activated-carbon filtration (BAC) on its own achieved the greatest reduction in SDS-DBPs. Ozonation alone caused adverse effects by promoting THM, HAA, and TCNM formation. Ozonation and BAC filtration yielded better DOC removal (51%) than BAC filtration alone (41%). Considering the cost of ozonation, we suggest that when treating high cyanobacterial organic matter in water destined for a small-scale water distribution system, BAC biofiltration alone could be an efficient, economical option for reducing DBP precursors. If DOC removal needs to be improved, preceding ozonation could be incorporated.

Pif1 regulates telomere length by preferentially removing telomerase from long telomere ends.

Telomerase, a ribonucleoprotein complex, is responsible for maintaining the telomere length at chromosome ends. Using its RNA component as a template, telomerase uses its reverse transcriptase activity to extend the 3'-end single-stranded, repetitive telomeric DNA sequence. Pif1, a 5'-to-3' helicase, has been suggested to regulate telomerase activity. We used single-molecule experiments to directly show that Pif1 helicase regulates telomerase activity by removing telomerase from telomere ends, allowing the cycling of the telomerase for additional extension processes. This telomerase removal efficiency increases at longer ssDNA gaps and at higher Pif1 concentrations. The enhanced telomerase removal efficiency by Pif1 at the longer single-stranded telomeric DNA suggests a way of how Pif1 regulates telomerase activity and maintains telomere length.

Characterization of persistent virus-like particles in two acetate-fed methanogenic reactors.

The objective of this study was to characterize the morphology, size-distribution, concentration and genome size of virus-like particles (VLPs) in two acetate-fed Methanosaeta-dominated reactors to better understand the possible correlation between viruses and archaeal hosts. The study reactors were dominated by a single genus of acetoclastic methanogen, Methanosaeta, which was present at 6 to 13 times higher than the combined bacterial populations consisting of Proteobacteria, Firmicutes, and Bacteroidetes. Epifluorescent microscopy showed VLPs concentration of 7.1 ± 1.5 × 10(7) VLPs/ml and 8.4 ± 4.3 × 10(7) VLPs/ml in the two laboratory reactors. Observations of no detectable import of VLPs with the reactor feed combined long operational time since the last inocula were introduced suggests that the VLP populations were actively propagating in the reactors. Transmission electron microscopy images showed VLPs with morphology consistent with Siphoviridae in both reactors, and VLPs with morphologies consistent with Myoviridae in one of the reactors. The morphology, size-distribution and genome size of VLPs were distinct between reactors suggesting that unique viral populations inhabited each reactor, though the hosts of these VLPs remain unclear.

Nitrifying community structures and nitrification performance of full-scale municipal and swine wastewater treatment plants.

This study evaluated nitrification performance and microbial ecology of nitrifying sludge in two full-scale wastewater treatment plants (WWTPs) including a municipal WWTP treating 20mgNL(-1) of ammonium and a swine WWTP treating 220mgNL(-1) of ammonium. These two plants differed in both wastewater characteristics and operating parameters, such as influent COD, TKN, ammonium, hydraulic retention time, and solids retention time, even though both plants achieve >85% nitrification efficiency. By employing molecular techniques, including terminal restriction fragment length polymorphism, cloning-sequencing and phylogenetic analyses targeting the 16S ribosomal RNA and group specific ammonia-monooxygenase functional gene (amoA), microbial community structures of nitrifying sludge and their significance to nitrification performance were evaluated. The results reveal that for the municipal WWTP Nitrosomonas marina-like AOB (ammonia-oxidizing bacteria) and Nitrospira-like NOB (nitrite-oxidizing bacteria) were the ubiquitously dominant nitrifiers, while Nitrosomonas europaea-, Nitrosomonas oligotropha-, and Nitrosospira-like AOB and Nitrobacter- and Nitrospira-like NOB were the major nitrifying populations found in the swine WWTP. The observed dissimilar nitrifying populations prevailing in these two plants may be related to niche differentiation concerning ammonium concentrations, system operation, and salinity. Moreover, our results suggest that the swine nitrifying sludge, involving relatively diverse AOB and NOB populations that perform the same task but with distinct growth and survival characters, may allow communities to maintain nitrifying capabilities when conditions change such as sudden increases in ammonium concentrations as examined with nitrification kinetic batch tests.