Ovarian Development and Larval Release of the Hermit Crab Clibanarius eurysternus (Hilgendorf, 1879) (Decapoda: Anomura: Diogenidae) in Okinawa, Japan.

This study investigated ovarian development and larval release in the hermit crab Clibanarius eurysternus, which inhabits the upper intertidal zone on Okinawa, Japan. Adult females were collected monthly and had a high gonadosomatic index (GSI) from May to October. Oocytes at vitellogenic stages were observed from May to October. Some females had post-ovulatory follicles in the ovary and embryos on the abdomen (ovigerous females) in June and August, suggesting active ovarian development and larval release during these months (breeding season). When adult females were collected weekly according to the lunar cycle, a high GSI with oocytes at the tertiary yolk globule stage in the ovaries was maintained in all lunar phases. Since post-ovulated follicles were seen in ovaries collected around the last quarter and new moons, darkness at night is needed for spawning. Ovigerous females exposed to stepwise changes in hydrostatic pressure exhibited significantly higher synchrony between larval release and high pressure at night from the last quarter moon to the new moon, suggesting the importance of the tidal amplitude cycle in larval release under non-moonlight conditions. We concluded that the continuity of synchronous spawning and larval release of C. eurysternus are repeated under darkness and high tide at night during the breeding season.

Seasonality and Longevity of the Functional Chloroplasts Retained by the Sacoglossan Sea Slug Plakobranchus ocellatus van Hasselt, 1824 Inhabiting A Subtropical Back Reef Off Okinawa-jima Island, Japan.

Plakobranchus ocellatus is a sacoglossan sea slug that feeds on multiple algal species and retains chloroplasts as kleptoplasts for several months. The seasonal differences in the photosynthetic properties of kleptoplasts were examined in sacoglossans collected from a subtropical back reef off of Okinawa-jima (26°21'55"N 127°44'10"E) in 2017-2018. The effective quantum yield of photosystem II in kleptoplasts indicated that stronger ambient light causes more stress in kleptoplasts. The maximum quantum yields (QY) at 20°C, 30°C, and 40°C indicated that kleptoplasts were more functional in photosynthesis in winter than in spring or summer, whereas kleptoplasts may have the highest tolerance to high temperatures in summer. In the long-starvation experiment (LSE), the relative ratio of body weight (relW) linearly decreased and the sacoglossans died within 2 months in the total dark condition, whereas in the LSE with illumination, the animals survived up to 5 months. The time course for the decrease in the relative ratio of the QY (relQY) in the LSE indicated that the photosynthetic function was almost normal for 2 months, regardless of the presence or absence of illumination, after which time relQY gradually decreased to zero. In the field, P. ocellatus continuously took up new kleptoplasts that have suitable properties of photosynthetic ability for each season. In a subtropical environment, in which water temperatures vary from below 20°C to above 30°C, seasonal changes could cause a temporary shortage of algal food and affect the photosynthetic activity of P. ocellatus kleptoplast. Our results, however, indicated the kleptoplasts of P. ocellatus functioned normally for several months and maintained the presence of this sacoglossan in a subtropical environment throughout the year.