Astrometrically registered maps of H2O and SiO masers toward VX Sagittarii.

The supergiant VX Sagittarii is a strong emitter of both H2O and SiO masers. However, previous VLBI observations have been performed separately, which makes it difficult to spatially trace the outward transfer of the material consecutively. Here we present the astrometrically registered, simultaneous maps of 22.2 GHz H2O and 43.1/42.8/86.2/129.3 GHz SiO masers toward VX Sagittarii. The H2O masers detected above the dust-forming layers have an asymmetric distribution. The multi-transition SiO masers are nearly circular ring, suggesting spherically symmetric wind within a few stellar radii. These results provide the clear evidence that the asymmetry in the outflow is enhanced after the smaller molecular gas clump transform into the inhomogeneous dust layers. The 129.3 GHz maser arises from the outermost region compared to that of 43.1/42.8/86.2 GHz SiO masers. The ring size of the 129.3 GHz maser is maximized around the optical maximum, suggesting that radiative pumping is dominant.

Circadian regulation of low density lipoprotein receptor promoter activity by CLOCK/BMAL1, Hes1 and Hes6.

Low density lipoprotein receptor (LDLR) plays an important role in the cholesterol homeostasis. We examined the possible circadian regulation of LDLR and mechanism(s) underlying it. In mice, blood glucose and plasma triglyceride, total and high density lipoprotein cholesterol varied distinctively throughout a day. In addition, LDLR mRNA oscillated in the liver in a functional clock-dependent manner. Accordingly, analysis of human LDLR promoter sequence revealed three putative E-boxes, raising the possible regulation of LDLR expression by E-box-binding transcription factors. To test this possibility, human LDLR promoter reporter constructs were transfected into HepG2 cells and the effects of CLOCK/BMAL1, Hes1, and Hes6 expression were analyzed. It was found that positive circadian transcription factor complex CLOCK/BMAL1 upregulated human LDLR promoter activity in a serum-independent manner, while Hes family members Hes1 and Hes6 downregulated it only under serum-depleted conditions. Both effects were mapped to proximal promoter region of human LDLR, where mutation or deletion of well-known sterol regulatory element (SRE) abolished only the repressive effect of Hes1. Interestingly, hes6 and hes1 mRNA oscillated in an anti-phasic manner in the wild-type but not in the per1-/-per2 -/- mouse. Comparative analysis of mouse, rat and human hes6 genes revealed that three E-boxes are conserved among three species. Transfection and site-directed mutagenesis studies with hes6 reporter constructs confirmed that the third E-box in the exon IV is functionally induced by CLOCK/BMAL1. Taken together, these results suggest that LDLR expression is under circadian control involving CLOCK/BMAL1 and Hes family members Hes1 and Hes6.