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1.
J Vet Sci ; 17(3): 323-30, 2016 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-26726018

RESUMO

Infectious coryza (IC) is an infectious disease caused by Avibacterium (Av.) paragallinarum. IC is known to cause economic losses in the poultry industry via decreased egg production in layers. Between 2012 and 2013, Av. paragallinarum was isolated from seven chicken farms by Chungbuk National University. We identified Av. paragallinarum, the causative pathogen of IC by polymerase chain reaction (PCR) and serovar serotype A, by multiplex PCR. Antibiotic sensitivity tests indicated that a few field-isolated strains showed susceptibility to erythromycin, gentamicin, lincomycin, neomycin, oxytetracycline, spectinomycin, and tylosin. A serological survey was conducted to evaluate the number of flocks that were positive for Av. paragallinarum by utilizing a HI test to determine the existence of serovar A. Serological surveys revealed high positivity rates of 86.4% in 2009, 78.9% in 2010, 70.0% in 2011, and 69.6% in 2012. We also challenged specific pathogen-free chickens with isolated domestic strains, ADL121286 and ADL121500, according to the measured efficacy of the commercial IC vaccine, PoulShot Coryza. We confirmed the effectiveness of the vaccine based on relief of clinical signs and a decreased re-isolation rate of ADL121500 strain. Our results indicate IC is currently prevalent in Korea, and that the commercial vaccine is effective at protecting against field strains.


Assuntos
Galinhas , Infecções por Haemophilus/veterinária , Haemophilus paragallinarum/fisiologia , Doenças das Aves Domésticas/epidemiologia , Vacinas Virais/farmacologia , Animais , Infecções por Haemophilus/epidemiologia , Infecções por Haemophilus/prevenção & controle , Infecções por Haemophilus/virologia , Haemophilus paragallinarum/genética , Haemophilus paragallinarum/imunologia , Reação em Cadeia da Polimerase/veterinária , Doenças das Aves Domésticas/prevenção & controle , Doenças das Aves Domésticas/virologia , República da Coreia/epidemiologia , Análise de Sequência de DNA/veterinária , Organismos Livres de Patógenos Específicos
2.
Avian Pathol ; 44(1): 28-34, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25510852

RESUMO

Chicken parvovirus (ChPV) is one of the causative agents of viral enteritis. Recently, the genome of the ABU-P1 strain of ChPV was fully sequenced and determined to have a distinct genomic composition compared with that of vertebrate parvoviruses. However, no comparative sequence analysis of coding regions of ChPVs was possible because of the lack of other sequence information. In this study, we obtained the nucleotide sequences of all genomic coding regions of three ChPVs by polymerase chain reaction using 13 primer sets, and deduced the amino acid sequences from the nucleotide sequences. The non-structural protein 1 (NS1) gene of the three ChPVs showed 95.0 to 95.5% nucleotide sequence identity and 96.5 to 98.1% amino acid sequence identity to those of NS1 from the ABU-P1 strain, respectively, and even higher nucleotide and amino acid similarities to one another. The viral proteins (VP) gene was more divergent between the three ChPV Korean strains and ABU-P1, with 88.1 to 88.3% nucleotide identity and 93.0% amino acid identity. Analysis of the putative tertiary structure of the ChPV VP2 protein showed that variable regions with less than 80% nucleotide similarity between the three Korean strains and ABU-P1 occurred in large loops of the VP2 protein believed to be involved in antigenicity, pathogenicity, and tissue tropism in other parvoviruses. Based on our analysis of full-length coding sequences, we discovered greater variation in ChPV strains than reported previously, especially in partial regions of the VP2 protein.


Assuntos
Galinhas/virologia , Variação Genética , Parvovirus/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Primers do DNA/genética , Dados de Sequência Molecular , Reação em Cadeia da Polimerase/veterinária , Análise de Sequência de DNA/veterinária , Homologia de Sequência , Especificidade da Espécie
3.
J Vet Sci ; 15(4): 579-82, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24962418

RESUMO

Eggs exhibiting eggshell apex abnormalities (EAA) were evaluated for changes in shell characteristics such as strength, thickness, and ultrastructure. Mycoplasma synoviae (MS) infection was confirmed by serological assay along with isolation of MS from the trachea and oviduct. Changes in eggshell quality were shown to be statistically significant (p < 0.01). We also identified ultrastructural changes in the mammillary knob layer by Scanning Electron Microscopy. While eggs may seem to be structurally sound, ultrastructural evaluation showed that affected eggs do not regain their former quality. In our knowledge, this is the first report describing the occurrence of EAA in Korea.


Assuntos
Casca de Ovo/ultraestrutura , Infecções por Mycoplasma/veterinária , Mycoplasma synoviae/fisiologia , Doenças das Aves Domésticas/microbiologia , Animais , Galinhas , Casca de Ovo/microbiologia , Microscopia Eletrônica de Varredura/veterinária , Infecções por Mycoplasma/microbiologia , República da Coreia
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