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Virology ; 432(1): 155-61, 2012 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-22749880

RESUMO

The complete genomic RNAs of two Korean sacbrood virus (SBV) strains, which infect the honey bee, Apis mellifera, were sequenced. The two sequences (AmSBV-Kor19, AmSBV-Kor21) were distinguished by the presence or absence of a PstI restriction site. These strains comprised of 8784 bp and 8835 bp; contained a single large ORF (179-8707 and 179-8758) encoding 2843 and 2860 amino acids, respectively. Deduced amino acid sequences comparison with some insect viruses showed that regions of helicase, protease and RdRp domains; structural genes were located at the 5' end and non-structural genes at the 3' end. Multiple sequence alignment showed that AmSBV-Kor19 was missing a section between nucleotides 2311 and 2361 (present in SBV-UK and CSBV) but was similar to that of the Korean SBV strain that infects A. cerana (AcSBV-Kor). The differences in the AmSBV-Kor19 strain may be the result of the virus adapting to a different host.


Assuntos
Abelhas/virologia , Genoma Viral , Vírus de RNA/genética , Vírus de RNA/isolamento & purificação , RNA Viral/genética , Análise de Sequência de DNA , Animais , Desoxirribonucleases de Sítio Específico do Tipo II/metabolismo , Dados de Sequência Molecular , Fases de Leitura Aberta , Filogenia , Vírus de RNA/classificação , República da Coreia , Homologia de Sequência de Aminoácidos
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