Evaluating the Effect of NanoFilled Composite Restorations on the Wear Resistance of Posterior Teeth: An RCT.

Background: Dental restorations play a crucial role in preserving the structural integrity and function of posterior teeth. However, wear resistance remains a significant concern for these restorations. Materials and Methods: In this RCT, 120 participants with posterior tooth restoration needs were randomly assigned to two groups. Group A received conventional composite restorations, while Group B received NanoFilled Composite restorations. The restorations were placed according to standardized protocols. Wear resistance was assessed using a custom-designed chewing simulator, and the material wear was quantified using a profilometer. Patient-reported outcomes and clinical evaluations were also recorded at regular intervals over a 24-month follow-up period. Results: The wear resistance of the NanoFilled Composite restorations (Group B) was significantly higher than that of the conventional composite restorations (Group A) after 24 months of follow-up (P < 0.05). Profilometer measurements revealed that Group B had an average wear depth of 0.15 mm, while Group A had an average wear depth of 0.25 mm. Patient-reported outcomes indicated higher satisfaction and comfort in Group B. Clinical evaluations also demonstrated that Group B restorations had better retention and marginal integrity. Conclusion: This RCT demonstrates that NanoFilled Composite restorations exhibit superior wear resistance compared to conventional composite restorations when used in posterior teeth.

Comparative Evaluation of Debris Expulsion Beyond Apex During Re-root Canal Treatment by Utilizing Two Re-treatment Rotary Files and Two Reciprocating Files: An In-Vitro Study.

AIM: This study aimed to assess and compare debris quality expelled beyond the apex during re-treatment using two reciprocating and two re-treatment rotary files. MATERIALS AND METHODS: A total of 80 healthy human mandibular premolars excised for orthodontic therapy were selected. Distilled water was used to sterilize the teeth before they were preserved. The canals were widened and coronal access was created so that the F2 ProTaper universal system could be used. For the obturation, we utilized gutta-percha cones and AH plus sealant. All samples were cone-beam computed tomography (CBCT) recorded. Teeth were separated into four groups (n=20); Group 1: ProTaper re-treatment, Group 2: Mtwo re-treatment, Group 3: WaveOne gold, and Group 4: Reciproc. The re-instrumented process included collecting the extruded debris in Eppendorf tubes and then drying it off by evaporating the distilled water. The weight of the tube with debris was subtracted from the weight of the tube without debris to get the dry debris weight. Post-retreatment CBCT was recorded for all samples. Mean apical debris extrusion values were obtained, after which necessary statistical analysis was obtained using SPSS Statistics, version 21 (IBM Corp., Armonk, NY) to obtain the results. A one-way analysis of variance (ANOVA) and the student t-test were utilized for statistical analysis to compare the different experimental groups. Post-hoc Bonferroni tests were used to compare several groups. RESULT: No statistical difference (p>0.05) was identified in the filler material that was left over across all groups. The reciprocating files discharged more material compared with re-treatment file systems. CONCLUSION: The Reciproc files expelled more waste than WaveOne gold, ProTaper re-treatment, and Mtwo re-treatment file systems. Reciprocating file systems showed better cleaning efficiency compared to the re-treatment file systems.

Effects of Blood Contamination and Decontamination Protocol on Reverse Torque Value of Abutment Screws in Dental Implants: An In Vitro Study.

Background and Objective: Loosening of abutment screws in dental implants is a mechanical complication that affects prosthetic treatments and hence, patient satisfaction. Blood contamination of abutment screws may play a role in this phenomenon. However, only limited research attention has been given to this issue. In the present study, we determined the effect of blood contamination and decontamination protocol on the reverse torque value (RTV) of abutment screws. Materials and Methods: A questionnaire-based survey was sent to 210 implantologists requesting feedback on their attitude to the blood contamination issue and the decontamination protocols used. The survey responses were used in a selection of the decontamination solutions that were used in the subsequent in vitro study on the effects of blood decontamination protocol on the RTV of abutment screws. Thus, three study groups were used (n = 20 abutment screws in each group): Group 1 (control group; blood-contaminated screws); Group 2 (screws decontaminated with 5.25% sodium hypochlorite (NaOCl) solution); and Group 3 (screws decontaminated with normal saline solution (0.9%)). Then, each of the connections were subjected to thermocycling, and RTVs of the screw were measured using a digital torque meter. Intragroup and intergroup RTVs were analyzed for significance using analysis of variance (ANOVA) and Tukey's honestly significant difference (HSD) tests. Results: 48% of the implantologists responded to the survey; 80% of them were concerned with blood contamination in the implant connection, especially before abutment loading and 85% of them used either chlorhexidine solution or normal saline solution as the decontamination agent. The mean RTV for Group 2 screws (30.27 ± 2.8 N.cm) was significantly greater than that for Group 3 screws (26.02 ± 1.99 N.cm) which, in turn, was significantly greater than that for Group 1 screws (23.64 ± 1.84 N.cm). Conclusion: Decontamination of blood-covered connections using 5.25% NaOCl solution or normal saline solution restores the RTV of abutment screws. This finding may have clinical relevance in that the decontaminated screws may contribute to the low incidence of screw loosening and, ultimately, improved patient satisfaction.

Radiographic Evaluation of the Gap between Cemented Post and Remaining Gutta-Percha in Endodontically Treated Teeth Performed by Undergraduate Students: A Retrospective Cross-Sectional Study.

The coronal seal in root canal-treated teeth may be compromised depending on the accuracy of post space preparation and post cementation along with remaining gutta-percha. Root canal treatment can be compromised by endotoxins released by the coronal bacteria as a result of microleakage. The study was conducted by undergraduate students to measure the gap between the cemented post and residual gutta-percha. In total, 217 endodontically treated teeth were evaluated with intraoral peri-apical radiographs. Based on the intraoral periapical radiographic examination in the CS-R4 program, Group I had no gap, Group II had a gap of >0 to 2 mm, and Group III had a gap of more than 2 mm between the end of the cemented post and the remaining gutta-percha. In total, 40% (n = 87) of the teeth had no gap, 59% (127) had a gap of >0 to 2 mm, and 1% (n = 3) had a gap of more than 2 mm between the cemented post end and remaining gutta. Chi square test revealed a significant difference in the gap between the post and remaining gutta-percha between males and females students (p < 0.001). In terms of the gap between the cemented post end and the residual gutta-percha, the root canal treated teeth with post and core by undergraduates were clinically acceptable.

The Effect of a Digital Manufacturing Technique, Preparation Taper, and Finish Line Design on the Marginal Fit of Temporary Molar Crowns: An In-Vitro Study.

The aim of this study is to investigate the combined effect of a digital manufacturing technique (subtractive vs. additive), preparation taper (10° vs. 20° TOC), and finish line (chamfer vs. shoulder) on the marginal adaptation of temporary crowns following cementation with a compatible temporary cement. Four mandibular first molar typodont teeth were prepared for full coverage crowns with standard 4 mm preparation height as follows: 10° TOC with the chamfer finish line, 10° TOC with the shoulder finish line, 20° TOC with the chamfer finish line and 20° TOC with the shoulder finish line. Each of the four preparation designs were subdivided into two subgroups to receive CAD/CAM milled and 3D-printed crowns (n = 10). A total of 80 temporary crowns (40 CAD/CAM milled and 40 3D-printed) were cemented to their respective die using clear temporary recement in the standard cementation technique. The samples were examined under a stereomicroscope at ×100 magnification following calibration. Linear measurements were performed at seven equidistant points on each axial surface and five equidistant points on each proximal surface. One-way ANOVA analysis and Tukey HSD (Honestly Significance Difference) were performed. The best marginal fit was seen in group 8, while the poorest fit was noted in group 2. Shoulder finish lines and 10° TOC resulted in higher marginal gaps, especially in CAD/CAM milled group. The selection of 3D-printed crowns may provide a better marginal fit within the range of clinical acceptability. Marginal gaps were within clinical acceptability (50 and 120 µm) in all groups except group 2.

Anatomical Evaluation of Root and Root Canal Morphology of Permanent Mandibular Dentition among the Saudi Arabian Population: A Systematic Review.

This study aimed to conduct a compendious review of root canal morphology of "permanent mandibular teeth in different regions of Saudi Arabia" to obtain a large sample representing the total population. A detailed search through the databases Web of Science, Scopus, and PubMed was conducted following the PRISMA guidelines. The data were analyzed based on the following inclusion criteria: original full-length original articles that reported the variables of interest "(number of roots, number of canals, Vertucci's classification system and C-shaped canals or mid-mesial canals)" of the mandibular teeth and conducted on Saudi subjects. The retrieved data were presented as frequencies and percentages. The results revealed that 56.6% of mandibular central incisors had one canal and Vertucci type I (56.6%), while 57.4% of the mandibular lateral incisors had one canal, with Vertucci types I and III most frequent. In mandibular canines, 91.8% had one canal and 8.2% had two canals. Most of the mandibular first premolars had one root (86.6%), while almost all mandibular second premolars (91.5%) had one canal, and 96.9% had Vertucci type I configuration. Among the mandibular first molars, three and four canals were prevalent in 58.7% and 40.6%, respectively. The majority of mesial roots had Vertucci type IV (60.6%), and most of distal roots had Vertucci type I (72.2%). Most of the mandibular second molars had three canals (87.3%) and showed Vertucci type IV (39.4%) canals for mesial roots and Vertucci type I (95.6%) for distal roots. The C-shaped canals were seen in 8% of first premolars and 9.8% of second molars. The middle mesial canal was found in 4.2% and 0.4% of first and second molars, respectively. This review could represent "the population of Saudi Arabia as the included samples were combined from different regions of the country." Some variations were noticed within the same group of teeth from different regions. However, the overall results of combined samples were comparable to the other international studies.

Evaluating the Effect of Tideglusib-Loaded Bioactive Glass Nanoparticles as a Potential Dentine Regenerative Material.

Dental pulp treatment is the least intrusive procedure currently available for preserving the vitality of the pulp. Several studies are underway to improve the bioactivity of pulp capping materials. Tideglusib isa potent anti-inflammatory, antioxidant, and a regenerative drug developed against Alzheimer's disease and has been shown to be effective in the treatment of dental cavities. However, its bioactive properties encapsulated within the nanoparticles as a component of pulp capping material are largely unknown. In this study, tideglusib-loaded bioactive glass nanoparticles were synthesized (tideglusib-BgNPs) and mixed at various concentrations into the calcium silicate cement to testits physiomechanical and bioactivitiescompared with biodentine (control). The calcium silicate cement with 10wgt% tideglusib-BgNPs showed comparable physiomechanical properties to that of biodentine. Additionally, the assessment of cytotoxicity and bioactivity (cell proliferation, wound healing, and cell migration assays) showed increased bioactivity in terms of better wound healing, increased proliferation, and better migration of human dental pulp stem cells than biodentine. These findings suggest new opportunities to use tideglusib-BgNPs in pulp therapy.

Evaluation of Micro-Tensile Bond Strength of Fibre Post with Titanium Dioxide Nanoparticles as Fillers in Experimental Dental Composite Resin.

BACKGROUND: The clinical success of post-core restorations is determined by the composite utilized and the strength of the post-core adhesion. The effectiveness of titanium dioxide nanoparticles (TiO2 NPs) as a multifunctional material with photo-induced activities and better mechanical characteristics are observed as particle size is reduced to under 50 nm. AIM: The purpose of this study is to determine the bond strength of fibre-reinforced composite (FRC) posts with TiO2 NP as fillers and to compare it with conventional composite resin core material. MATERIALS AND METHODS: 30 single-rooted mandibular premolars were selected and routine root canal procedures were done. A quantity of 5% TiO2 NPs were synthesized and added as silanized filler to the experimental composite resin. Post space was prepared and fibre-reinforced composite (FRC) post luting was performed. The specimens were then grouped into the following groups: Group I consisted of the experimental composite resin containing 5% TiO2 fillers, Group II consisted of core X flow, and Group III consisted of Multicore Flow. All test groups were submitted for thermocycling. After this, the samples were tested for micro tensile bond strength. A stereomicroscope with a magnification of 20× was used to examine the fractured surfaces. The data were analysed using one-way ANOVA and Tukey HSD tests. RESULTS: Statistical analysis revealed that Group I showed the highest mean bond strength value of 35.6180 Mpa. The results obtained with Group III showed the lowest mean bond strength value of 19.4690 Mpa. Adhesive failures were identified by stereomicroscopy of the fractured surfaces. CONCLUSION: The experimental composite resin comprising 5% TiO2 NP had a greater bond to the FRC post than other materials tested.

Effect of Different Intracanal Medicaments on the Viability and Survival of Dental Pulp Stem Cells.

BACKGROUND: Stem cells play an important role in the success of regenerative endodontic procedures. They are affected by the presence of medicaments that are used before the induction of bleeding or the creation of a scaffold for endodontic regeneration. This study examines the effects of different intracanal medicaments on the viability and survival of dental pulp stem cells at different doses and over different exposure times. METHODS: Dental pulp stem cells were cultured from healthy third molar teeth using the long-term explant culture method and characterized using flow cytometry and exposed to different concentrations of calcium hydroxide, doxycycline, potassium iodide, triamcinolone, and glutaraldehyde, each ranging from 0 (control) to 1000 µg/mL. Exposure times were 6, 24, and 48 h. Cell viability was measured using the MTT assay, and apoptosis was measured using the Annexin V-binding assay. RESULTS: All medicaments significantly reduced cell viability at different concentrations over different exposure times. Calcium hydroxide and triamcinolone favored cell viability at higher concentrations during all exposure times compared to other medicaments. The apoptosis assay showed a significant increase in cell death on exposure to doxycycline, potassium iodide, and glutaraldehyde. CONCLUSION: The intracanal medicaments examined in our study affected the viability of dental pulp stem cells in a time and dose-dependent manner. They also adversely affected the survival of dental pulp stem cells. Further studies are needed to better understand the effect of prolonged exposure to medicaments according to clinical protocols and their effect on the stemness of dental pulp stem cells.

Efficacy of Three Commercially Available Desensitizers in Reducing Post-Operative Sensitivity Following Composite Restorations: A Randomized Controlled Clinical Trial.

One of the most widely used esthetic restorations in dentistry is composite. The widespread application of composites can be related to advancements in biomaterials. However, due to various factors, composites are commonly associated with dental sensitivity. Hence, the present study evaluates and compares the effectiveness of three desensitizing agents in reducing post-treatment sensitivity for Class I composite restoration. Eighty subjects with Class I cavities were selected according to the inclusion criteria, and a randomized, double-blind, controlled clinical trial was carried out. Twenty patients were randomly assigned to four groups: Group C (Control group), Group GL (Gluma group), Group SF (Shield Force Plus group), and Group TC (Telio CS group). The desensitizers were applied after Class 1 cavity preparation and acid etching in all the groups, except the Control group, and thereafter, composite restoration was completed in a conventional manner. Questionnaires were provided to all the participants to record the post-operative pain/sensitivity level according to the visual analogue scale (VAS) on intake of cold drinks, intake of hot drinks, and intake of sugar for different periods of time. Significant variation was observed between the three desensitizers for all three stimuli. However, no significant variations were seen with the various age groups and between the maxillary and the mandibular teeth at the different time periods. Group GL performed better than Group SF and Group TC. It can be proposed that the application of the desensitizers reduced the post-restorative sensitivity in the composite restorations and improved acceptance.

The Effect of Commercially Available Desensitizers on Bond Strength Following Cementation of Zirconia Crowns Using Self-Adhesive Resin Cement-An In Vitro Study.

The improvement of the tensile strength of zirconia crowns after the application of commercially available desensitizers can provide added advantages for the durability and strength of zirconia prostheses. We assessed the retention of zirconia crowns when Gluma, Shield Force Plus, and Telio CS desensitizers were used with resin luting cement. Four groups with ten specimens each (n = 10) were considered as Group 1 (Control group, with no desensitizer application before crown cementation with resin cement) and Groups 2, 3, and 4 (with a single coat of Gluma dentin desensitizer, Telio CS desensitizer, or Shield Force Plus desensitizer applied before crown cementation, respectively). Thermocycling was then carried out, and each group was tested to determine the associated retentive forces and type of failure. The data were statistically analyzed, which showed that the mean tensile-strength values were significantly higher in Group 2 (p-value = 0.001), Group 3 (p-value = 0.027), and Group 4 (p-value = 0.014), when compared with the Control group. Clinicians should consider the application of any of these three desensitizers, as they can successfully abate dentin hypersensitivity after tooth preparation, as well as increase the durability and strength of the zirconia prosthesis.

Structural Features of the Mental Foramen in a Saudi Subpopulation: A Retrospective CBCT Study.

INTRODUCTION: Accurate and precise knowledge about the position, size, and shape of the mental foramen (MF) are critical in avoiding procedural complications. The MF's anatomical features vary among different ethnic groups, and various radiographic techniques have been used to determine these variations. AIMS: To evaluate the MF's shape, vertical and horizontal positions, and distance from the border of the mandible. To evaluate the differences among genders as they pertain to the right and left sides of the mandible and research the bilateral symmetry regarding the same variables. MATERIALS AND METHODS: Cone beam computer tomography (CBCT) scans of 155 Saudi patients (69 males and 86 females) who visited the college of dentistry's clinics were obtained from the college database for this retrospective study. All the scans were analyzed by 3 calibrated examiners. The data collected was analyzed statistically, and results were obtained. RESULTS: The MF was located under the mandibular second premolar in 56.9% of cases, whereas in 26.9% of cases, it was located between the first and second mandibular premolar. The most prevalent position was below the level of the apices of the mandibular premolar teeth (87.2%). The round shape was most frequent (44.9%) compared to the H-oval (34.7%) and V-oval (20.4%). The V-oval shape was more frequent in males, while the H-oval shape was more frequent in females. The average distance from the center of the MF to the mandibular border was 14.03 ± 1.58 mm, with males exhibiting a greater distance than females. Overall, there were no significant differences between the bilateral symmetry and the right and left sides for all parameters. CONCLUSION: The most common position of the MF was under the root apex of the mandibular second premolar, with an average distance of about 14 mm from the border of the mandible. The position and shape of the MF were the same bilaterally in the majority of individuals.

Study of optimal conditions for growth and osteogenic differentiation of dental pulp stem cells based on glucose and serum content.

Dental pulp stem cells (DPSCs) have shown promising characteristics in terms of their proliferation and osteogenic differentiation potential, which could be of greater benefit in regenerative dentistry. However, obstacles remain in the in vitro cultivation of DPSCs, which significantly affect their growth and differentiating ability. Therefore in this study, we demonstrated the growth and osteogenic differentiation of DPSCs in the presence of media containing different combinations of serum and glucose to get an optimized combination of both. DPSCs were cultured in media containing combinations of low glucose (LG), low serum (LS), high glucose (HG), and high serum (HS). The proliferation and osteogenic differentiation were assessed in DPSCs cultured with these different combinations of culture conditions. High glucose high serum condition significantly inhibited the proliferation of DPSCs and also affected their clonogenic potential, as evidenced by colony-forming units. Irrespective of the serum content, high glucose in the media also decreased the osteogenic potential of DPSCs confirmed by functional staining, and downregulation of osteogenesis-related genes. High glucose content in the culture media affects the growth and differentiation potential of the DPSCs. Hence, the culture conditions for the DPSCs should be reconsidered to utilize their maximum potential.

Efficacy of Desensitizers in Reducing Post-preparation Sensitivity Prior to a Fixed Dental Prosthesis: A Randomized Controlled Clinical Trial.

AIMS: The aim of this article is to evaluate and compare the effectiveness of Gluma, Shield Force Plus, and Telio CS desensitizers, in reducing pre- and post-cementation sensitivity for complete coverage restorations. MATERIALS AND METHODS: The study was a double-blind, randomized, controlled clinical trial in which 56 patients requiring posterior three-unit fixed partial dentures were randomly assigned to four groups, each group with 14 patients (n = 14): Group C (Control group), Group GL (Gluma group), Group SF (Shield Force Plus group), and Group TC (Telio CS group). In the desensitizer groups, desensitizer application was performed following the manufacturer's directions immediately after tooth preparation (first visit), before metal restoration try-in (second visit, 2 weeks after the first visit), and before final cementation (third visit, 2 weeks after the second visit). Sensitivity levels were scored and evaluated using a visual analog scale (VAS), using cold test and electric pulp test (EPT) during the three visits before the cementation, and then over the phone 2 weeks after the final cementation. The data were statistically analyzed using one-way analysis of variance (ANOVA) followed by post-hoc Bonferroni and unpaired t-tests. RESULTS: One-way ANOVA revealed significant differences between the four groups. The post-hoc Bonferroni tests showed a significant decrease in the mean cold test scores from the first to third visit, with a p-value <0.001 for the GL, SF, and TS groups. The mean EPT scores also decreased significantly from the first to third visit (p< 0.001) for the GL and SF groups, whereas p = 0.023 for the TS group. Most of the subjects did not complain of any sensitivity post-cementation, except for one patient in Group TS. CONCLUSION: All three desensitizers were found to be effective in reducing pre- and post-cementation dentin sensitivity, as indicated by the consistent reduction in VAS scores throughout the visits.

Complete Obturation-Cold Lateral Condensation vs. Thermoplastic Techniques: A Systematic Review of Micro-CT Studies.

To prevent re-infection and provide a hermetic seal of the root canal system, an endodontist must aim to produce a void-free obturation. This review aimed to compare the completeness of root canal obturation between the two most prevalent methods-cold lateral condensation and warm gutta-percha techniques-using micro-CT (PROSPERO reg no. 249815). MATERIALS AND METHODS: A search of Scopus, Embase, PubMed (Medline via PubMed), and Web of Science databases was done without any time restriction according to the PRISMA protocol. Articles that compared both techniques and were published in English were included. Data was extracted and the risk of bias was assessed using an adapted tool based on previous studies. RESULTS: A total of 141 studies were identified by the search. Following the screening and selection of articles, 9 studies were included for review. Data was extracted manually and tabulated. Most studies had a moderate risk of bias. None determined operator skill in both methods before comparison. The data extracted from the included studies suggests that both techniques produce voids in the obturation. The thermoplasticized gutta-percha techniques may result in fewer voids compared to cold lateral condensation. CONCLUSION: Considering the limitations of the included studies, it was concluded that neither technique could completely obturate the root canal. Thermoplasticized gutta-percha techniques showed better outcomes despite a possible learning bias in favor of cold lateral condensation. Establishing operator skills before comparison may help reduce this bias.

Taurine Augments Telomerase Activity and Promotes Chondrogenesis in Dental Pulp Stem Cells.

BACKGROUND: Stem cell therapy has become an advanced and state-of-the-art procedure to regenerate lost tissues of the human body. Cartilage repair is a challenging task in which stem cells find potential application. One of the important biologic modifiers that can cause chondrogenic differentiation of stem cells is taurine. However, taurine has not been investigated for its effects on dental pulp derived stem cell (DPSC) chondrogenic differentiation. OBJECTIVE: The objective of the study was to investigate if taurine administration to DPSCs heralds chondrogenic differentiation as ascertained by expression of SOX9, COL2A1, ACAN, ELN, and COMP. The study also investigated if the differentiated cells synthesized glycosaminoglycans, a marker of cartilage formation. The study also aimed to assess proliferative activity of the cells after taurine administration by measuring the hTERT gene and protein expression. MATERIALS AND METHODS: DPSCs were obtained from a molecular biology laboratory and characterization of stem cell markers was done by flow cytometry. The cells were subjected to a MTT assay using various concentrations of taurine. Following this, hTERT gene and protein estimation was done in the control, telomerase inhibitor treated DPSC (TI-III), 10 µM taurine treated DPSC, and TI-III + 10 µM taurine treated DPSCs. A polymerase chain reaction was done to assess gene expression of SOX9, COL2A1, ACAN, ELN, and COMP genes and glycosaminoglycans were estimated in control cells, Induced DPSCs, induced and TI-III treated DPSCs, and 10 µM taurine treated DPSCs. RESULTS: DPSCs expressed CD73, CD90, and CD105 and did not express CD34, CD45, and HLA-DR, which demonstrated that they were mesenchymal stem cells. The MTT assay revealed that various concentrations of taurine did not affect the cell viability of DPSCs. A concentration of 10 µM of taurine was used for further assays. With regard to the hTERT gene and protein expression, the taurine treated cells expressed the highest levels that were statistically significant compared to the other groups. Taurine was also found to restore hTERT expression in telomerase inhibitor treated cells. With regard to chondrogenesis related genes, taurine administration significantly increased the expression of SOX9, COL2A1, ACAN, and ELN genes in DPSCs and caused a significant increase in glycosaminoglycan production by the cells. CONCLUSIONS: Taurine can be regarded a biologic modifier that can significantly augment chondrogenic differentiation of DPSCs and can find potential applications in regenerative medicine in the area of cartilage regeneration.

Antimicrobial Efficacy of Silver Nanoparticles as Root Canal Irrigant's: A Systematic Review.

Removal of microbes is imperative during endodontic therapy. Due to their antimicrobial property, silver nanoparticles have been used for endodontic irrigation of the root canals. The objective of the present study was to provide a qualitative analysis of the published literature assessing silver nanoparticles as root canal irrigants. A search of PubMed, SCOPUS, Web of Science, and Embase databases was done without any time restriction. Articles published in English were included. Data were extracted and the risk of bias was assessed. Of the 154 studies identified, after screening according to the inclusion criteria, five in vitro studies were included. The results indicate that silver nanoparticles have an anti-microbial effect to varying degrees depending on certain factors. Within the limitations of the present studies that have a moderate to low risk of bias, an antimicrobial effect of silver nanoparticles is observed. Silver nanoparticles have the potential to be used as endodontic irrigants, although their efficacy depends on particle size and the duration of contact which require further investigation.

Efficacy of photodynamic therapy, photobiomodulation and antimicrobial agent on the shear bond strength of composite restorations to hypomineralized teeth.

PURPOSE: The aim of this study was to investigate the adhesive bond strength and fracture analysis of resin based restorations to carious dentin of hypomineralized molars (HMs) after disinfecting with photodynamic therapy (PDT), Nd:YAG and chlorhexidine (CHX). MATERIALS AND METHODS: Sixty carious HM molars were investigated. After removal of all carious infected tissue, the cavities were condition with methylene blue initiated PDT, a 1064 nm wavelength Nd:YAG laser or 2.0% CHX gel. Non-carious dentin from HM was used as a control without performing any disinfection or conditioning technique. All samples were bonded using a one-step one-coat adhesive bond over the dentin surface, packed with composite and light cured. All specimens were subjected to thermocycling at 5°C and 60°C with a dwell time of 30 s for 10,000 cycles. Bonded specimens were placed on a universal testing machine with a flat loading head. The debonded surface were viewed under light optical microscope to estimate the type of failure including adhesive, cohesive, and admixed. RESULTS: The highest mean SBS was seen in the non-carious HM (control group). The mean score for control group was 17.64 ± 2.96 MPa. The lowest bond strength value was displayed for Group-PDT (11.37 ± 3.66 MPa) and Group-Nd:YAG (12.69 ± 2.45 MPa). Affected dentin disinfected with the Group-CHX (15.71 ± 3.04 MPa) bonded with composite showed statistically significantly higher SBS values compared to the laser groups (p < 0.05). No statistically significant difference was noted with specimens disinfected with PDT and Nd:YAG laser showed comparable SBS scores (p > 0.05). Fracture analysis showed equal incidence of adhesive and cohesive failures in control group and Group-CHX. Moreover, a significant number of failures in group-PDT were related to adhesive. Whereas in group 4, admixed type failures were common. CONCLUSION: Caries infected hypomineralized teeth demonstrated lower bond strength values compared to non-carious hypomineralized teeth. Adhesive bond strength of hypomineralized teeth was highest with chlorhexidine compared to other laser treatment strategies.

Evaluation of Root Canal Morphology of Maxillary First Premolars by Cone Beam Computed Tomography in Saudi Arabian Southern Region Subpopulation: An In Vitro Study.

INTRODUCTION: The aim of this study was to investigate the root canal morphology of maxillary first premolars in Saudi Arabian subpopulation by cone beam computed tomography (CBCT). METHODS: One hundred maxillary first premolars were collected from the College of Dentistry at Jazan University and different dental centers in the southern region of Saudi Arabia. These teeth were cleaned thoroughly and were mounted in a plastic artificial mandible jaw to mimic soft tissue which was then placed in a water container. The 3D scan images were obtained with CBCT imaging (3D Accuitomo170) and evaluated on the basis of the number of roots, number of canals, and root canal configuration using Vertucci's classification. RESULTS: Out of the 100 maxillary first premolars, the majority of teeth had 2 roots (61%) followed by (36%) one root. Two canals were the most predominant with 97% and then 3% three canals, and no tooth presented with one canal. Type IV canal configuration was most prevalent (75%) followed by Type V (13%). CONCLUSIONS: The CBCT provides enhanced and accurate information of root morphology, canal configuration, and its variations, thereby constituting an excellent alternative for radiographic diagnosis tool in the dental practice.

Comparison of Sagittal Condylar Guidance Determined by Panoramic Radiographs to the One Determined by Conventional Methods Using Lateral Interocclusal Records in the Saudi Arabian Population.

OBJECTIVES: The objectives of this study were to determine the condylar guidance by the conventional method using interocclusal records and by panoramic radiographs in healthy dentate patients and to compare the values obtained from panoramic radiographs with the values obtained by a conventional method. MATERIALS AND METHODS: Thirty healthy dentulous subjects of either sex with an age range of 20-40 years visiting Jazan University, College of Dentistry, Dental Clinics for replacement of missing teeth or crowns, were selected according to the inclusion criteria. Maxillary and mandibular casts were obtained and mounted on Whipmix 2240 articulator. Right and left lateral interocclusal records were then made in patients by base plate wax (Dentsply truwax baseplate). This record was transferred to articulator, and condylar values were determined. In all the cases, articulator was programmed. Left and right condylar values were also measured on digital radiographs and readings were recorded. Condylar guidance readings obtained from interocclusal records and those obtained from panoramic radiographic images were compared and analyzed statistically using the t-test. RESULTS: The condylar guidance values obtained from the interocclusal record method for both left and right sides were less when compared to the values obtained from tracing the panoramic radiographs (radiographic method). The difference in values of both the methods was highly significant. CONCLUSION: Although a significant amount of correlation was found between the two methods, better radiographic techniques should be further investigated.