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J Med Assoc Thai ; 91(11): 1677-80, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19127788

RESUMO

OBJECTIVES: To develop and apply the polymerase chain reaction with confronting two-pair primers (PCR-CTPP) for detection and identification of hemoglobin E (Hb E). MATERIAL AND METHOD: Fifty unrelated northern Thais were included in the present study. DNA was extracted from peripheral blood mononuclear cells and targeted to amplify by PCR-CTPP. The amplified product was analyzed and compared with the reference hemoglobin electrophoresis and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. RESULTS: The results validated a completely concordant among these three methods consisting of 74%, 24%, and 2% identified as normal, heterozygous, and homozygous Hb E type, respectively. CONCLUSION: Successful Hb E genotyping by PCR-CTPP was introduced. It allows for confirming and simultaneously detection with other thalassemia mutations.


Assuntos
Primers do DNA , Hemoglobina E/análise , Reação em Cadeia da Polimerase/instrumentação , Polimorfismo de Fragmento de Restrição/genética , Talassemia beta/diagnóstico , DNA , Amplificação de Genes , Genótipo , Hemoglobina E/genética , Humanos , Reação em Cadeia da Polimerase/métodos , Tailândia , Talassemia beta/genética
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