Green titanium dioxide (TiO2) nanoparticles assisted biodegradation of anthracene employing Serratia quinivorans HP5.

The anthracene biodegradation potential of Serratia quinivorans HP5 was studied under a controlled laboratory environment. The green TiO2 nanoparticles (NPs) synthesized from Paenibacillus sp. HD1PAH was used to accelerate the biodegradation process. The synergistic application of TiO2 NPs and S. quinivorans HP5 resulted in a reduction of anthracene concentration by 1.2 folds in liquid-medium and 1.5 folds in contaminated soil. Gas-chromatography and mass-spectrometric investigation showed the production of four anthracene derivatives, namely 1,2-anthracene dihydrodiol, 6,7-benzocoumarin, anthrone, and 9,10-anthraquinoneat the termination of experimental periods. Furthermore, bacterial biomass increased by 23.3 folds in the presence of TiO2 NPs, and overall soil enzyme activities were enhanced by 4.2 folds in the treated samples. In addition, there was a negative correlation observed between the biomass of S. quinivorans HP5 and the concentrations of anthracene, suggesting the involvement of bacterium in anthracene biodegradation processes. The degradation pathway of anthracene revealed its transformation into the less toxic compound 9,10-anthraquinone. Overall, this study elucidates a novel biodegradation pathway for anthracene and highlights the potential of nano-assisted bacterial remediation as a promising approach for environmental cleanup.

Ag Nanoparticle Incorporated Guar Gum-Sodium Alginate-I-Carrageenan Tribiopolymer Blended Cloth Waste Lint Extracted Cellulose Nanocrystal Antimicrobial Composite Film.

A biopolymer-based formulation for robust and active food packaging material was developed. This material consisted of a blend of three biopolymers (guar gum-sodium alginate-i-carrageenan) reinforced by cellulose nanocrystals (CNC) alongside the integration of silver nanoparticles (AgNPs) with varying sizes. The CNC utilized in this process was derived from cloth waste lint (CWL) generated from a household cloth dryer machine. This CNC synthesis underwent a series of solvent treatments to yield the CNC used in the composite. CNC and AgNPs were incorporated into the tribiopolymeric blend matrix to construct a nanocomposite film that showed excellent tensile strength (∼90 MPa). The nanocomposite film also exhibited antimicrobial activity against Escherichia coli ATCC 25922 and Bacillus cereus MTCC 1272. In this report, it was demonstrated that the zone of inhibition against E. coli and B. cereus depends on the variation of size and amount of AgNPs inside the polymeric matrix. The practical applicability of such a film was also demonstrated by applying it to sliced bread and the enhancement of the shelf life of the raped bread was compared with a control. Thus, the guar gum-sodium alginate-i-carrageenan tribiopolymer blend with a cloth waste lint extracted cellulose nanocrystal composite film is antimicrobial, hence, an excellent candidate as an active packaging film.

Banana fibre-chitosan-guar gum composite as an alternative wound healing material.

Bio-composites, which can be obtained from the renewable natural resources, are fascinating material for use as sustainable biomaterials with essential properties like biodegradable, bio-compatibility as well cyto-compatibility etc. These properties are useful for bio-medical including wound healing applications. In this study, fibre obtained banana pseudo stem of banana plant, which is otherwise wasted, was used as a material along with chitosan and guar gum to fabricate a banana fibre-biopolymer composite patch. The physiochemical properties of the patches were examined using Fourier Transformed Infra-red spectrophotometer (FT-IR), tensile tester, Scanning Electron Microscope (SEM), contact angle tester, swelling and degradation studies. We further demonstrated that a herbal drug, Nirgundi could be loaded to the patch showed controlled its release at different pHs. The patch had good antibacterial property and supported proliferation of mouse fibroblast cells. The study thus indicates that banana fibre-chitosan-guar gum composite can be developed into an alternative wound healing material.

Animal derived biopolymers for food packaging applications: A review.

It is essential to use environment-friendly, non-toxic, biodegradable and sustainable materials for various applications. Biopolymers are derived from renewable sources like plants, microorganisms, and agricultural wastes. Unlike conventional polymers, biopolymer has a lower carbon footprint and contributes less to greenhouse gas emission. All biopolymers are biodegradable, meaning natural processes can break them down into harmless products such as water and biomass. This property is of utmost importance for various sustainable applications. This review discusses different classifications of biopolymers based on origin, including plant-based, animal-based and micro-organism-based biopolymers. The review also discusses the desirable properties that are required in materials for their use as packaging material. It also discusses the different processes used in modifying the biopolymer to improve its properties. Finally, this review shows the recent developments taking place in using specifically animal origin-based biopolymer and its use in packaging material. It was observed that animal-origin-based biopolymers, although they possess unique properties however, are less explored than plant-origin biopolymers. The animal-origin-based biopolymers covered in this review are chitosan, gelatin, collagen, keratin, casein, whey, hyaluronic acid and silk fibroin. This review will help in renewing research interest in animal-origin biopolymers. In summary, biopolymer offers a sustainable and environment-friendly alternative to conventional polymers. Their versatility, biocompatibility will help create a more sustainable future.

Cobalt-substituted ZnS QDs: a diluted magnetic semiconductor and efficient photocatalyst.

Recently, understanding the origin of induced magnetic characteristics in transition metal atom-doped QDs has been a major focus owing to their potential applications in the area of spintronic devices. A detailed experimental and theoretical investigation was conducted to understand the physical properties of Co-doped ZnS QDs containing different weight percentages of Co atoms [CoxZn1-xS (x = 0.00, 0.03, 0.06, and 0.09)], prepared using chemical co-precipitation techniques. X-ray diffraction studies proved that all the prepared QDs formed an extremely pure cubic zinc blende crystallographic phase free of contaminants. The validation of the quantum dot nature of all the samples was provided by the HRTEM images, BET studies, and blue shift in the absorption spectra. Both the obtained FTIR and PL spectra at room temperature also confirmed the phase purity of the prepared QDs. The observed weak ferromagnetic behavior of the doped samples was due to the presence of p-d hybridization between the 3d levels of Co2+ ions and 3p levels of S2- ions of the host ZnS QDs. Hysteresis loops that were obtained at room temperature validated this weak ferromagnetic nature. These obtained results were also supported theoretically using DFT calculations. FDTD simulations provided a detailed explanation for the observed blue shift in the absorption spectra originating from the quantum confinement effect of doped and undoped ZnS QDs. The dielectric properties of all the samples were examined properly, and it was also found that the grain boundaries contributed effectively to providing the dielectric response. The doped ZnS sample containing more Co dopants at low frequencies showed a progressive rise in polarisation loss. In addition, Co-doped ZnS QDs are efficient photocatalysts. A pH-dependent photodegradation test of ciprofloxacin (CIP) antibiotic was conducted using 9% Co-doped ZnS QDs. It was observed that 9% Co-doped ZnS nanocatalysts has sufficient capability to degrade CIP to around 94.7% in a solution of pH 10 within one hour. Therefore, besides showing photocatalytic effects, Co-doped ZnS QDs act as ideal dilute magnetic semiconductors (DMSs) and will undoubtedly become excellent candidates for the microelectronics industry because of their special ability to exhibit spin-dependent magneto-electro-optical properties that find use in spin-polarized light-emitting diodes, solid-state lasers, and spin-transistor devices.

Nanocrystalline Ni-Zn spinel ferrites: size-dependent physical, photocatalytic and antioxidant properties.

The physical properties of nanomagnetic particles are expected to be highly dependent on their size. In this study, besides the promising applications of nanocrystalline Ni-Zn spinel ferrites in the area of photocatalysis and free radical scavenging, we present a detailed study with appropriate scientific explanations on the role of size change in modifying and tuning the microstructural, optical and magnetic properties. Three nanostructured Zn0.3Ni0.7Fe2O4 samples of different particle sizes were prepared via the chemical co-precipitation method. Crystallographic phase purity and formation of the spinel cubic phase for all the samples were tested by X-ray diffraction studies. The magnetic properties of the as-synthesized ferrite nanoparticles have been examined thoroughly at 5 K and 300 K. Emergence of superparamagnetic behavior has been observed for the sample with the smallest size ferrite nanoparticles (ZNF-1). The photocatalytic efficiency of all the nanocatalysts was tested on methylene blue (MB) dye and the smallest sized nanocatalyst (ZNF-1) was identified as the most efficient catalyst in degrading MB dye under light illumination. The degradation efficiency was found to decrease with increasing mean particle size of the prepared samples. The antioxidant properties of the prepared ferrite samples were also studied. Here, too, the ZNF-1 sample with the smallest sized nanoparticles exhibited maximum scavenging of free radicals compared to other samples. Hence, the present study clearly demonstrates that smaller-sized Ni-Zn spinel ferrites are efficient materials for tuning the physical properties as well as for use in photocatalytic and antioxidant applications.

Biogenic Carbon Quantum Dots: Synthesis and Applications.

The new class of nanomaterials termed carbon dots: a quasi-spherical nanoparticle having a size less than 10 nm, possesses some unique characteristics like good aqueous solubility, colloidal stability, resistance to photobleaching, and fluorescence tunability, resulting in the unfolding of their various properties and their usage in different applications. Materials that are naturally derived or produced by living organisms are termed 'biogenic'. Over the past few years, there has been a gradual increase in the use of naturally derived materials in synthesizing carbon dots. Green precursors or biogenic materials are of low cost, readily available, renewable, and environmentally benign. Most importantly, they provide essential benefits not found in synthesized carbon dots. This review focuses on the use of biogenic materials for the synthesis of biogenic carbon dots developed in the past five years. It also briefly explains different synthetic protocols used, along with some significant findings. Thereafter, an overview of the use of biogenic carbon dots (BCDs) in different applications like chemo and biosensors, drug delivery, bioimaging, catalysis and energy applications, etc., is discussed. Thus biogenic carbon dots are future sustainable materials that are now fast replacing conventional carbon quantum prepared from other sources.

Grain Characteristics, Moisture, and Specific Peptides Produced by Ustilaginoidea virens Contribute to False Smut Disease in Rice (Oryza sativa L.).

The fungus Ustilaginoidea virens, the causative agent of false smut in rice (Oryza sativa L.), is responsible for one of the severe grain diseases that lead to significant losses worldwide. In this research, microscopic and proteomic analyses were performed by comparing U. virens infected and non-infected grains of the susceptible and resistant rice varieties to provide insights into the molecular and ultrastructural factors involved in false smut formation. Prominent differentially expressed peptide bands and spots were detected due to false smut formation as revealed by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis (2-DE) SDS-PAGE profiles and were identified using liquid chromatography-mass spectrometry (LC-MS/MS). The proteins identified from the resistant grains were involved in diverse biological processes such as cell redox homeostasis, energy, stress tolerance, enzymatic activities, and metabolic pathways. It was found that U. virens produces diverse degrading enzymes such as ß-1, 3-endoglucanase, subtilisin-like protease, putative nuclease S1, transaldolase, putative palmitoyl-protein thioesterase, adenosine kinase, and DNase 1 that could discretely alter the host morphophysiology resulting in false smut. The fungus also produced superoxide dismutase, small secreted proteins, and peroxidases during the smut formation. This study revealed that the dimension of rice grain spikes, their elemental composition, moisture content, and the specific peptides produced by the grains and the fungi U. virens play a vital role in the formation of false smut.

The role of selenium and nano selenium on physiological responses in plant: a review.

Selenium (Se), being an essential micronutrient, enhances plant growth and development in trace amounts. It also protects plants against different abiotic stresses by acting as an antioxidant or stimulator in a dose-dependent manner. Knowledge of Se uptake, translocation, and accumulation is crucial to achieving the inclusive benefits of Se in plants. Therefore, this review discusses the absorption, translocation, and signaling of Se in plants as well as proteomic and genomic investigations of Se shortage and toxicity. Furthermore, the physiological responses to Se in plants and its ability to mitigate abiotic stress have been included. In this golden age of nanotechnology, scientists are interested in nanostructured materials due to their advantages over bulk ones. Thus, the synthesis of nano-Se or Se nanoparticles (SeNP) and its impact on plants have been studied, highlighting the essential functions of Se NP in plant physiology. In this review, we survey the research literature from the perspective of the role of Se in plant metabolism. We also highlight the outstanding aspects of Se NP that enlighten the knowledge and importance of Se in the plant system.

Acute toxicity study of onion peel-derived gold nano-bioconjugate.

The acute anti-inflammatory activity of onion peel-derived gold nano-bioconjugate was already established earlier. The current study was aimed to investigate the acute oral toxicity of onion peel-derived gold nano-bioconjugate (GNBC) for safe therapeutic utilization in vivo. The acute toxicity study was carried out in female mice for 15 days and showed no mortality and any abnormal complications. The lethal dose (LD50) was evaluated and found to be higher than 2000 mg/kg. After 15 days, animals were euthanized and hematological, and biochemical analyses were performed. In all hematological and biochemical assays, treated animals did not show significant toxicity when compared to the control group. The body weight, behavior, and histopathological studies showed that GNBC is nontoxic. Thereby, the results suggest that onion peel-derived gold nano-bioconjugate GNBC can be utilized for therapeutic applications in vivo.

Anthracene removal potential of green synthesized titanium dioxide nanoparticles (TiO2-NPs) and Alcaligenes faecalis HP8 from contaminated soil.

Anthracene biodegradation potential has been studied in liquid culture and soil microcosm environment by employing green synthesized TiO2 nanoparticles (NPs) and Alcaligenes faecalis HP8. The bacterium was isolated from crude oil contaminated soil, while TiO2 nanoparticles were synthesized using Paenibacillus sp. HD1PAH and Cyperus brevifolius which have PAHs remediation abilities. The dual application of TiO2 nanoparticles and Alcaligenes faecalis HP8 decreases anthracene concentration up to 21.3% in liquid at the end of 7 days and 37.9% in the soil treatments after completion of 30 days. Besides, the GC-MS analysis revealed production of five metabolites including 1,2-anthracenedihydrodiol; 6,7-benzocoumarin; 3-hydroxy-2-naphthoic acid; salicylic acid and 9,10-anthraquinone at different time interval of the treatments. Anthracene degradation pathway confirms the breakdown of three ring anthracene to one ring salicylic acid. Additionally, soil dehydrogenase, urease, alkaline phosphatase, catalase and amylase activities increased up to 4.09 folds, 8.6 folds, 4.4 folds, 3.6 folds and 2.1 folds respectively after the combined treatments of TiO2 nanoparticles and Alcaligenes faecalis HP8. The bacterial biomass and residual anthracene concentration were found to be negatively correlated. Finally, the study brings into light a novel anthracene biodegradation pathway and provides a new dimension in nano assisted bacterial remediation.

Biogenic carbon dot-based fluorescence-mediated immunosensor for the detection of disease biomarker.

The dynamic interplay of nanotechnology and immunoassay has unlocked an arena for developing next-generation techniques to contribute to detecting disease biomarkers. Herein, the work establishes the strategic fabrication of an immunosensor by sandwich technique based on the fluorescence phenomenon of carbon dots for the detection of the disease biomarker VEGF (Vascular Endothelial Growth Factor). VEGF, a biomarker for angiogenesis, is considered cancerous if found in elevated levels in the blood, and so is paramount for early detection of disease. Carbon dots derived from a biogenic source were synthesized employing a green microwave-assisted method followed by conjugating with a detection antibody, human immunoglobulin G (IgG), via EDC-NHS amidation reaction. On the other hand, the VEGF biomarker was immobilized onto the capture antibody. The detection antibody tagged with the fluorescent probe is employed as a bridge to connect with the VEGF biomarkers bound to the capture antibody. The response to different concentrations of VEGF biomarkers was recorded in terms of the fluorescence intensity of the carbon dots. The fluorescence immunosensor could exhibit a wide linear range of 0.1 fg/mL to 10 pg/mL with a low detection limit of 5.65 pg/mL towards VEGF. The potentiality of this designed immunosensor was qualitatively assessed with human blood plasma samples, showing promising results, thereby upholding the applicability of carbon dots as fluorescent labels in immunoassay techniques.

Guar gum-sodium alginate nanocomposite film as a smart fluorescence-based humidity sensor: A smart packaging material.

Perishable packed foods are easily damaged by the change in relative humidity. In this work, we demonstrate that guar gum- sodium alginate blending with glucose-glycerol carbon dots nanocomposite film can be used to detect relative humidity. The fabricated nanocomposite film was an excellent smart sensor based on the fluorescence 'on-off' mechanisms against humidity. The study demonstrates that at different relative humidity conditions, such as 11 %, 33 %, 75.30 %, 84 %, and 97 %, there is a change in the fluorescence of biocomposite films under UV light. The practical feasibility of the biocomposite developed film was tested in real conditions by placing a piece of bread with high humidity conditions wrapped with the developed nanocomposite film. It was observed that under such conditions, marked quenching of fluorescence was observed and hence detection of humidity was possible. Hence, the fabricated nanocomposite film can monitor the packed food freshness using just a UV light source. Such biopolymer nanocomposite is potential materials and may find application as smart packaging materials, especially as food packaging materials.

Ecological risk assessment of priority PAHs pollutants in crude oil contaminated soil and its impacts on soil biological properties.

Polycyclic aromatic hydrocarbons (PAHs) are one of the major toxic constituents of crude oil and therefore, an understanding on PAHs associated risks and their relationship with soil biological parameters are necessary for adopting effective risk-based and site specific remediation strategies in the contaminated soil. Here, risks evaluation of eight detected PAHs in terms of toxic equivalent concentration (TEQC), benzo(a)pyrene equivalent (BaPeq), contamination factor (CF), pollution load index (PLI), hazard quotient (HQ), hazard index (HI), toxic unit for individual PAHs (TU) and PAHs mixture (TUm) have been evaluated. Besides, the effect of PAHs contamination on soil biological properties has also been investigated and correlated with PAHs concentrations. The TEQc of eight PAHs was recorded in the range of 0.06-5.0 mg kg-1 soil, whereas the BaPeq value was 25.3 mg kg-1 soil which exceed the permissible limit. Similarly, CF (85.5-1668.2), PLI (322.8), HQ (311.7-8340.9), HI (26,443.8), TU (227.9-3821.6) and TUm(7916.2) also exceed the permissible values for non-toxic conditions indicating carcinogenic risk for humans. Besides, activities of soil dehydrogenase, urease, alkaline-phosphatase, catalase, amylase and cellulase were decreased by 1.5-2.3 folds in the contaminated soil than control. The results of Pearson's correlation matrix also established negative impact of PAHs on the soil's biological properties.

Insight into carbon quantum dot-vesicles interactions: role of functional groups.

Understanding carbon quantum dot-cell membrane interaction is essential for designing an effective nanoparticle-based drug delivery system. In this study, an attempt has been made to study the interaction involving phosphatidylcholine vesicles (PHOS VES, as model cell membrane) and four different carbon quantum dots bearing different functional groups (-COOH, -NH2, -OH, and protein bovine serum albumin coated) using various tools such as PL behavior, surface charge on vesicles, QCM, ITC, TEM, LSV, and FTIR. From the above studies, it was observed that the -NH2 terminating carbon dots were capable of binding strongly with the vesicles whereas other functional groups bearing carbon dots were not significantly interacting. This observation was also supported by direct visual evidence as shown by transmission electron microscopy, which shows that the polyethyleneimine carbon dot (PEICD) bearing -NH2 functionality has greater affinity towards PHOS VES. The mechanistic insight presented in the paper indicates greater possibility of higher H-bonding, signifying better interaction between -NH2 functionalized carbon dots and PHOS VES supported by FTIR, QCM, ITC and TEM. Moreover, the transport of neurotransmitters (which are generally amine compound) in neurons for cellular communication through synapse is only possible through vesicular platforms, showing that in our body, such interactions are already present. Such studies on the nano-bio interface will help biomedical researchers design efficient carbon-based nanomaterial as drug/gene delivery vehicles.

Insights into Anti-Inflammatory Activity and Internalization Pathway of Onion Peel-Derived Gold Nano Bioconjugates in RAW 264.7 Macrophages.

Green synthesis of nanoparticles plays an important role in their efficient therapeutic effects in various biomedical applications. Here, we prepared gold nano bioconjugates (GNBCs) from the ethyl acetate fraction of onion peels and investigated their anti-inflammatory activity in lipopolysaccharide-stimulated RAW 264.7 macrophages. The GNBCs were characterized by UV-visible spectroscopy, dynamic light scattering, and transmission electron microscopy. Comparative studies have been conducted among GNBCs, fractionate alone [onion peel drug (OPD)], and the standard drug dexamethasone in various anti-inflammatory assays. It was observed that GNBCs showed comparatively good therapeutic efficacy than the fractionate alone. At the lowest 10 µg/mL concentration, the GNBC and OPD exhibited 70.86 and 91.98% of reactive oxygen species production, 10.88 and 20.97 ng/µL of nitrite production, 337 and 378 pg/mL of TNF-α production, 27.1 and 30.64 pg/mL of IL-6 production, respectively, by maintaining a satisfactory cell viability. Moreover, to understand the mechanistic pathway of GNBCs in their entry into the macrophages, their localization, and duration, uptake studies have been performed where a caveolar-mediated endocytosis pathway is found to be prominent. Hence, this study will lead to the development of cheap, green synthesis of nano bioconjugates and their role in inflammation.

Green Synthesis of Gold Nano-bioconjugates from Onion Peel Extract and Evaluation of Their Antioxidant, Anti-inflammatory, and Cytotoxic Studies.

Plant secondary metabolites such as flavonoids demonstrate high degrees of antioxidant, anti-inflammatory, and anticancer activities. Among flavonoids, quercetin plays an important role in inflammation by downregulating the level of various cytokines. Thereby, in this work, onion (Allium cepa) peel was successfully utilized for the synthesis of gold nano-bioconjugates acting as a natural therapeutic drug. In this process, crude onion peel extract was first divided into different fractionates, namely, ethyl acetate, butanol, methanol, and water, and they were subjected to various preliminary studies of antioxidant activities. The ethyl acetate fractionate shows high antioxidant activities in all the assays. The bioactive components were identified and found to contain a high amount of quercetin as confirmed by liquid chromatography with tandem mass spectrometry and high-performance liquid chromatogrpahy. Three gold nano-bioconjugates were prepared with different concentrations of the ethyl acetate fractionate. Various biochemical anti-inflammatory assays were carried out and compared with the active ethyl acetate fraction of the onion peel drug (OPD). The cytotoxicity of the nano-bioconjugate system and the OPD was checked in the myoblast L6 cell line from skeletal muscle tissues to evaluate the toxicity. All the three nano-bioconjugates A, B, and E demonstrated high percentages of cell viability, viz., 73.07, 72.3, and 69.15%, respectively, at their highest concentration of 200 µg/mL. The OPD also showed 88.56% cell viability with no toxic effects in the myoblast L6 cell line from skeletal muscle tissues. The reactive oxygen species reduction of nano-bioconjugate B showed a marked reduction of 76.77% at a maximum concentration of 200 µg/mL, whereas the OPD showed 68.17%. Hence, through this work, a cheap source of nano-bioconjugates is developed, which can act as a potent antioxidant and anti-inflammatory agent and are more active in comparison to the OPD alone.

Carbon dot based nucleus targeted fluorescence imaging and detection of nuclear hydrogen peroxide in living cells.

Investigation of the intracellular generation of H2O2, one of the most important reactive oxygen species (ROS), is crucial for preventing various diseases since it is closely linked with different physiological and complex cell signaling pathways. Despite the development of various fluorescent probes, the majority of the fluorescent probes cannot move across the nuclear membrane. However, detection of the nuclear level of H2O2 is very important since it can directly cause oxidative DNA damage which ultimately leads to various diseases. Therefore, in this study, p-phenylenediamine based carbon quantum dots (B-PPD CDs) have been synthesized and integrated with 4-formylbenzeneboronic acid as a doping agent for the detection of H2O2. The detection mechanism showed that, upon exposure to H2O2, the fluorescence of the B-PPD CDs was immediately quenched. Further investigation has been done in the in vitro RAW 264.7 cell line by both exogenous and endogenous exposure of H2O2 to demonstrate the feasibility of the method. It is shown successfully that the exogenous presence and endogenous generation of H2O2 in RAW 264.7 cells can be detected using B-PPD CDs. The limit of detection (LOD) was determined to be 0.242 µM. The development of such imaging probes using carbon quantum dots will lead to live-cell imaging as well as ROS detection.

Green synthesis of gold nanoparticles using an antiepileptic plant extract: in vitro biological and photo-catalytic activities.

Gold nanoparticles are one of the widely used metallic nanoparticle having unique surface plasmon characteristic, offers major utility in biomedical and therapeutic fields. However, chemically synthesized nanoparticle creates toxicity in the living organisms and contradicts the eco-friendly and cost-effective nature. So, developing greener synthetic route for synthesis of gold nanoparticle using natural materials is an enthralling field of research for its effectiveness in synthesizing eco-friendly, non-toxic materials. Moreover, biological components attached as stabilizing agent can exert its own effect along with the advantages of nanoparticle conjugation. In this work, we used for the first time methanolic leaf extract of Moringa oleifera as this fraction of M. oleifera exerts a neuroactive modulation against seizure as evidenced by earlier literature. The green gold nanoparticles synthesized were characterized by different characterization tools, dynamic light scattering and transmission electron microscopy techniques etc. Prepared nanoparticles were biologically (antioxidant, antimicrobial and blood cytotoxicity) characterized to screen their further utility in therapeutic strategies. Characteristics and activities of green gold nanoparticles were compared with conventional citrate stabilized gold nanoparticles. It was observed that green gold nanoparticles prepared using M. oleifera show less cytotoxicity and helps in regeneration of neuronal cells in animal model study. It establishes the fact that conjugation of different plant extract fraction for stabilization of gold nanoparticle may be responsible factor for enhancement of bioactive nature of green gold nanoparticle. In addition, the green gold nanoparticle show efficient photo-catalytic efficiency. Development of such bioactive gold nanoparticles will lead to functional materials for biomedical and therapeutic applications.

Phytochemical-Based Nanomedicine for Advanced Cancer Theranostics: Perspectives on Clinical Trials to Clinical Use.

In the current chapter, a new strategic compilation of phytochemicals with potent antitumor properties has been addressed, most importantly focusing on cell cycle arrest and apoptotic signaling mechanism. A promising approach in tumor prevention is to eliminate cancer cells preferably via cell cycle arrest and programmed cell death with lesser harm to neighboring normal cells. Cancer cells have a survival advantage to escape apoptosis and relentlessly divide to proliferate, gearing up the cell cycle process. Recently, the use of phytochemical-derived conjugated chemotherapeutic agents has increased dramatically owing to its biocompatibility, low cytotoxicity, low resistance, and dynamic physiochemical properties discriminating normal cells in the treatment of various cancer types. For decades, biomedical investigations have targeted cell cycle and apoptotic cell death mechanism as an effective cancer-killing tool for systemically assessing the potential biological interactions of functional phytocompounds compared to its synthetic counterparts during their complete life cycles from entry, biodistribution, cellular/molecular interactions to excretion. Newly emerging nanotechnology application in anticancer drug formulations has revolutionized cancer therapy. Tissue-specific phyto-nanomedicine plays a vital role in advanced cancer diagnostics using liposome, micelle, and nanoparticles as a precise and effective delivery vehicle. This chapter specifically focuses on the therapeutic phytomolecules approved by the Food and Drug Administration (FDA, USA) along with phyto-chemopreventives currently on clinical trials (Phase-I/II/III/IV). Besides, detailed coverage is given to the FDA-approved nanotechnology-based formulations only in the areas of cancer theranostics via cell cycle arrest and apoptotic pathways including present challenges and future perspectives.