G protein-mediated FMRFamidergic modulation of calcium influx in dissociated heart muscle cells from squid, Loligo forbesii.

The actions of the neuropeptide FMRFamide (Phe-Met-Arg-Phe-NH2) on the L-type (ICa,L) and T-type (ICa,T) calcium currents were investigated in muscle cells dissociated from the heart of squid, Loligo forbseii. The heart muscle cells could be divided into type I and type II cells, on the basis of morphological differences in the dissociated myocytes. FMRFamide induced a substantial block of the L-type calcium current seen in type I cells; this inhibition was rapid, reversible and dose dependent (IC50 = 0.1 microM). FMRFamide induced an increase in the amplitude of the L-type calcium current in the type II heart muscle cells, but had no effect on the T-type calcium current in either type of dissociated heart muscle cell, even at concentrations much higher than those found to affect the L-type calcium current. Internal dialysis of isolated type I heart muscle cells with guanosine 5'-O-(3-thiotriphosphate (GTPgammaS, 100 microM), a non-hydrolysable GTP analogue, mimicked the FMRFamide inhibition of the Ca2+ current and occluded any further FMRFamide-induced inhibition. Internal dialysis of these cells with guanosine 5'-O-(2-thiodiphosphate) (GDPbetaS, 100 microM) reduced the FMRFamide-induced inhibition of the peak Ca2+ current. The inhibitory effects of FMRFamide were abolished by pre-incubation of the cells with pertussis toxin (200 ng ml-1). The activation kinetics of ICa,L were not affected by FMRFamide application, nor by internal perfusion with GTPgammaS, and the FMRFamide-induced reduction in ICa,L was not relieved by large depolarising prepulses. These data indicate that FMRFamide can modulate ICa,L, but not ICa,T, in squid heart muscle cells, and that the underlying G protein pathway is dissimilar to that commonly associated with transmitter modulation of channel activity. The FMRFamide-modulated increase in ICa,L seen in the type II heart muscle cells was not mediated by a PTX-sensitive G protein pathway.

Synaptic interactions between crista hair cells in the statocyst of the squid Alloteuthis subulata.

Intracellular injections of the fluorescent dye Lucifer yellow into the various cell types within the anterior transverse crista segment of the statocyst of squid revealed that the primary sensory hair cells and both large and small first-order afferent neurons have relatively simple morphologies, each cell having a single, unbranched axon that passes directly into the small crista nerve that innervates the anterior transverse crista. However, the small first-order neurons have short dendritic processes occurring in the region of the sensory hair cells. The secondary sensory hair cells have no centripetal axons, but some have long processes extending from their bases along the segment. Simultaneous intracellular recordings from pairs of the different cell types in the anterior transverse crista segment demonstrated that electrical coupling is widespread; secondary sensory hair cells are coupled electrically along a hair cell row, as are groups of primary sensory hair cells. Secondary sensory hair cell also are coupled to neighboring small first-order afferent neurons. However, this coupling is rectifying in that it only occurs from secondary sensory hair cells to first-order afferent neurons. Direct electrical stimulation of the small crista nerve to excite the efferent axons revealed efferent connections to both the primary sensory hair cells and the small first-order afferent neurons. These efferent responses were of three types: excitatory or inhibitory postsynaptic potentials and excitatory postsynaptic potentials followed by inhibitory postsynaptic potentials. The functional significance of the cell interactions within the crista epithelium of the statocyst of squid is discussed and comparisons drawn with the balance organs of other animals.

Voltage-dependent conductances in cephalopod primary sensory hair cells.

Cephalopods, such as sepia, squid, and octopus, show a well-developed and sophisticated control of balance particularly during prey capture and escape behaviors. There are two separate areas of sensory epithelium in cephalopod statocysts, a macula/statolith system, which detects linear accelerations (gravity), and a crista/cupula system, which detects rotational movements. The aim of this study is to characterize the ionic conductances in the basolateral membrane of primary sensory hair cells. These were studied using a whole cell patch-clamp technique, which allowed us to identify five ionic conductances in the isolated primary hair cells; an inward sodium current, an inward calcium current, and three potassium outward currents. These outward currents were distinguishable on the basis of their voltage-dependence and pharmacological sensitivities. First, a transient outward current (IA) was elicited by depolarizing voltage steps from a holding potential of -60 mV, was inactivated by holding the cell at -40 mV, and was blocked by 4-aminopyridine. A second, voltage-sensitive, outward current with a sustained time course was identified. This current was not blocked by 4-aminopyridine nor inactivated at a holding potential of -40 mV and hence could be separated from IA using these protocols. A third outward current that depended on Ca2+ entry for its activation was detected, this current was identified by its sensitivity to Ca2+ channel blockers such as Co2+ and Cd2+ and by the N-shaped profile of its current-voltage curve. Inward currents were studied using cesium aspartate solution in the pipette to block the outward currents. Two inward currents were observed in the primary sensory hair cells. A fast transient inward current, which is presumably responsible for spike generation. This inward current appeared as a rapidly activating inward current; this was strongly voltage dependent. Three lines of evidence suggest that this fast transient inward current is a Na+ current (INa). First, it was blocked by tetrodotoxin (TTX); second, it also was blocked by Na+-free saline; and third, it was inactivated when primary hair cells were held at a potential more than -40 mV. The sustained inward current was not affected by TTX and was increased in amplitude 5 min after equimolar Ba2+ replaced Ca2+ as a charge carrier. This inward current also was blocked after external application of 2 mmol/l Co2+ or Cd2+. Furthermore, this current was reduced significantly in a dose-dependent manner by nifedipine, suggesting that it is an L-type Ca2+ current (ICa).

Ionic currents in identified swimmeret motor neurones of the crayfish Pacifastacus leniusculus

Ionic currents from freshly isolated and identified swimmeret motor neurones were characterized using a whole-cell patch-clamp technique. Two outward currents could be distinguished. A transient outward current was elicited by delivering depolarizing voltage steps from a holding potential of -80 mV. This current was inactivated by holding the cells at a potential of -40 mV and was also blocked completely by 4-aminopyridine. A second current had a sustained time course and continued to be activated at a holding potential of -40 mV. This current was partially blocked by tetraethylammonium. These outward currents resembled two previously described potassium currents: the K+ A-current and the delayed K+ rectifier current respectively. Two inward currents were also detected. A fast transient current was blocked by tetrodotoxin and inactivated at holding potential of -40 mV, suggesting that this is an inward Na+ current. A second inward current had a sustained time course and was affected neither by tetrodotoxin nor by holding the cell at a potential of -40 mV. This current was substantially enhanced by the addition of Ba2+ to the bath or when equimolar Ba2+ replaced Ca2+ as the charge carrier. Furthermore, this current was significantly suppressed by nifedipine. All these points suggest that this is an L-type Ca2+ current. Bath application of nifedipine into an isolated swimmeret preparation affected both the frequency of the swimmeret rhythm and the duration of power-stroke activity, suggesting an important role for the inward Ca2+ current in maintaining a regular swimmeret rhythmic activity in crayfish.

State-dependent responses of two motor systems in the crayfish, Pacifastacus leniusculus.

The expression of both swimmeret and postural motor patterns in crayfish (Pacifastacus leniusculus) were affected by stimulation of a second root of a thoracic ganglion. The response of the swimmeret system depended on the state of the postural system. In most cases, the response of the swimmeret system outlasted the stimulus. Stimulation of a thoracic second root also elicited coordinated responses from the postural system, that outlasted the stimulus. In different preparations, either the flexor excitor motor neurones or the extensor excitor motor neurones were excited by this stimulation. In every case, excitation of one set of motor neurones was accompanied by inhibition of that group's functional antagonists. This stimulation seemed to coordinate the activity of both systems; when stimulation inhibited the flexor motor neurones, then the extensor motor neurones and the swimmeret system were excited. When stimulation excited the flexor motor neurones, then the extensor motor neurones and the swimmeret system were inhibited. Two classes of interneurones that responded to stimulation of a thoracic second root were encountered in the first abdominal ganglion. These interneurones could be the pathway that coordinates the response of the postural and swimmeret systems to stimulation of a thoracic second root.

A separate local pattern-generating circuit controls the movements of each swimmeret in crayfish.

1. Within an abdominal segment, the motor output from the segmental ganglion to the swimmerets consists of coordinated bursts of impulses in the separate pools of motor neurons innervating the left and right limbs. This coordinated motor pattern features alternating (out-of-phase) bursts of impulses in the power-stroke (PS) and return-stroke (RS) motor axons that innervate each swimmeret. PS bursts on both sides of each segment occur simultaneously (in-phase), and so RS bursts on both sides are also in-phase. 2. With all intersegmental connections interrupted, isolated abdominal ganglia were able to sustain the normal swimmeret motor pattern of alternating PS/RS activity that was bilaterally in-phase. 3. After an isolated ganglion was surgically bisected down the midline, the isolated hemiganglia that resulted could produce stable, coordinated alternation of PS and RS bursts. 4. The neuropeptide proctolin could induce rhythmic oscillations of membrane potential in swimmeret neurons when spiking was blocked by tetrodotoxin (TTX). For neurons within the same hemiganglion, these oscillations retained the same phase relations they displayed in controls, but the oscillations of neurons in different hemiganglia became uncoordinated. 5. Synaptic transmission between swimmeret neurons in the same hemiganglion persisted in the presence of TTX. Swimmeret interneurons that could activate the pattern-generating circuitry under control conditions could induce membrane-potential oscillations in swimmeret neurons of the same hemiganglion when TTX was present. 6. We conclude that a separate hemisegmental pattern-generating circuit controls the rhythmic PS and RS movements of each swimmeret. Each circuit is located in the same hemiganglion as the population of motor neurons that innervates the local swimmeret. Graded transmission is sufficient to coordinate the timing of oscillatory activity within the hemisegmental circuitry. These hemisegmental circuits are coupled by intersegmental and bilateral coordinating pathways that are dependent on sodium action potentials for their operation.

Electrical coupling between primary hair cells in the statocyst of the squid, Alloteuthis subulata.

Intracellular recordings were made from primary sensory hair cells located on the dorsal side of the anterior crista segment of the squid statocyst. These hair cells were electrophysiologically identified by the occurrence of an antidromic action potential after electrical stimulation of the crista nerve. Two types of subthreshold, depolarising potentials were observed in the primary sensory hair cells. Firstly, those due to efferent inputs onto the primary hair cells and secondly those correlated one-to-one with action potentials in neighbouring primary hair cells. The former depolarising potentials could be blocked by bath applied cobalt, indicating chemical transmission, while the latter could not. Injection of a depolarising or hyperpolarising current into a primary hair cell depolarised or hyperpolarised, respectively, a neighbouring primary hair cell implying that the hair cells are electrically coupled with an electrical coupling coefficient of up to 0.4.

Interaction and synchronization between two abdominal motor systems in crayfish.

1. Extracellular and intracellular recordings from an isolated thoraco-abdominal preparation of the crayfish, Pacifastacus leniusculus, demonstrate that the swimmeret and the abdominal positioning systems can at times be spontaneously coordinated with each other. 2. Two forms of coordination were encountered between these two motor systems. First, some flexor and extensor motor neurons can burst in phase with the swimmeret power-stroke motor neurons. Second, when the flexor motor neurons displayed irregular bursting, the swimmeret rhythm was often inhibited. 3. Both of these two forms of coordination between the swimmeret and the abdominal positioning systems can be induced by depolarization of certain abdominal interneurons. 4. Bath application of oxotremorine increases the frequency of the swimmeret rhythm in a dose-dependent manner. The threshold concentration for this effect is 10(-8) M, and it persists for as long as oxotremorine is present in the bathing solution. 5. At a concentration of 10(-5) M, oxotremorine also induces slow rhythmic activity in the abdominal positioning system consisting of opposite firing between the flexor and extensor motor neurons. 6. Bath application of 10(-5) M oxotremorine also induces two types of interaction between these two abdominal motor systems. In cycle-by-cycle coordination the flexor motor neurons and one extensor motor neuron display rhythmic activity in phase with that of power-stroke motor neurons of the swimmeret system. A slow coordination also occurs with an inhibition of the swimmeret rhythm during the extensor bursts and an excitation during the flexor bursts. 7. Injection of similar doses of oxotremorine into the haemolymph of intact crayfish produces rhythmic abdominal movements that are comparable to the fictive pattern induced in the isolated preparation.

Fictive locomotion in the fourth thoracic ganglion of the crayfish, Procambarus clarkii.

Bath application of muscarinic agonists induced rhythmic motor activity in an in vitro preparation of the thoracic nervous system of the crayfish, Procambarus clarkii. In 70% of the cases, the rhythm was organized into 1 of the 2 normal patterns: "backward" walking or "forward" walking. In the rest (30%), the ganglion produced either a series of bursts of impulses or no rhythm at all, just an increase in the tonic activity. When it was isolated from all ascending and descending afferents, the fourth thoracic ganglion was still able to generate rhythmic motor output during bath application of muscarinic agonists. In certain motor neurons, muscarinic agonists induced plateau potentials. Under these conditions, some of these motor neurons were able to change the period of the motor pattern, which might suggest that these motor neurons were part of the central pattern generator (CPG) for locomotion. In the presence of 5 x 10(-6)M TTX, the membrane potential of these motor neurons continued to oscillate with organized rhythmic membrane potential oscillations into 1 of the 2 patterns. Under these conditions, current injection into certain motor neurons demonstrated that they continued to affect the CPG. Two classes of walking leg interneurons have been found. First, there are those with a sustained membrane potential: injection of a steady depolarizing current into some of these interneurons induced rhythmic activity in all thoracic motor nerves, even in the absence of any pharmacological activation. Second, there are those with an oscillating membrane potential: these seemed to enable silent motor neurons to be involved in an ongoing rhythm.

Synaptic connections between motor neurons and interneurons in the fourth thoracic ganglion of the crayfish, Procambarus clarkii.

1. A new preparation of the thoracic nervous system of the crayfish, Procambarus clarkii, has been developed, in which it is possible to work with identified members of motor neuronal pools. 2. In such a preparation, it is possible to dissect all specific proximal motor nerves (protractor, retractor, anterior elevator, posterior elevator, and depressor). Motor neurons innervating the four proximal muscles of the fourth walking leg have been identified both physiologically and anatomically by staining the recorded motor neuron with Lucifer yellow through the microelectrode. 3. By the use of cobalt chloride, we have mapped the distribution of somata of all motor neurons within the fourth thoracic ganglion that innervate the different groups of muscles controlling the movement of the fourth walking leg. 4. Most motor neurons innervating the same muscle seem to be electrically coupled, except some depressor motor neurons. 5. Motor neurons innervating antagonist muscles are linked by inhibitory connections. These connections are reciprocal for protractor and retractor motor neurons but usually not reciprocal between elevator and depressor motor neurons. 6. Walking interneurons were identified as neurons without axons in any motor nerve, which modified the motor neuronal activity. Some of them have been injected with Lucifer yellow. 7. Some interneurons make synaptic connections only with antagonist motor neurons that control the movement of one joint. Probably their functional role is to reinforce or to limit the antagonism between each pair of antagonist motor neurons. 8. Other interneurons make synaptic connections with motor neurons innervating muscles controlling different leg joints. These interneurons may play a role in generating the motor patterns that underlie forward and backward walking.

Induction of rhythmic activity in motoneurons of crayfish thoracic ganglia by cholinergic agonists.

In a crayfish thoracic ganglion preparation, it has been possible to induce rhythmic motoneuron (MN) activity by bath application of cholinergic agonists such as oxotremorine or pilocarpine (10(-6) and 10(-5) M respectively). Intracellular recordings of the MN and injection of current pulses demonstrate that these cholinergic agonists are powerful inducers of regenerative properties in MNs some of them being part of the central generator for locomotion.