Transcriptional activity of telomerase complex in CD34- stem cells of cord blood in dependence of preparation time.

The aim of the study was to determine whether the expression of telomerase subunits encoding genes changes during the process of cord blood preparation. It should establish if the commonly accepted 24 hours time interval in stem cells kriopreservation procedure significantly influences their immortalization and so decreases the "quality" of cord blood stem cells. Investigation includes 69 women. Spontaneous labour was the inclusion condition. The material was collected at birth after clamping of umbilical cord by direct vasopuncture. CD34- cells were extracted from cord blood (MACS, Miltenyi Biotec; Bisley, Surrey, UK). The expression profile of telomerase activators and inhibitors encoding genes was determined using HG_U133A oligonucleotide microarray (Affymetrix). We used a real-time quantitative RT-PCR assay to quantify the telomerase TERT, hTR and TP1 subunits mRNA copy numbers in CD34- cells in 0, 6, 12 and 24 hours after cord blood collection. We observed significant decrease of numbers of copies of TERTA+B mRNA within the successive hours of observation. Significant decrease of numbers of TERTA mRNA copies was confirmed after 24 hours. However, we observed significant increase of numbers of copies of TERTB mRNA after 6 hours of observation. Similar level was maintained during another 6h. The significantly lower number of copies of TERTB mRNA was observed after 24h. We also observed significant increase of number of copies of TERT mRNA after 6 hours. Number of copies of TERT mRNA significantly decreased after another 6h, remaining, however, on a higher then initial one. The significant lower number of copies of TERT mRNA was observed 24h after delivery. The possible explanation of those results is discussed in the paper.

[Continuous amnioinfusion in premature rupture of membranes]. / Zastosowanie amnioinfuzji ciaglej po przedwczesnym odplynieciu plynu owodniowego.

Results of premature rupture of membranes treatment including administration of continuous amnioinfusion procedure using amniotal catheter in 18 pregnancies between 23 and 28 weeks of gestation are presented in the study. Only a few early treatment complications and high rate of clinical success in the aspect of fetal surveillance were found. Clinical evidence of intra-amniotal infection after 2-6 weeks of therapy had become cesarean section cause in most cases of the study group. Authors have observed 17 live birth (one case of placental ablation excluded) with birth weight in the range of 950-2210 g (1540 +/- 230 g) with Apgar score 5-7. In the early neonatal period 7 cases of intensive care and 2 neonatal deaths were found.

[Usefulness of the pulse oximetry in diagnosis of fetal distress during labor]. / Przydatnosc pulsoksymetrii plodowej w diagnostyce niedotlenienia plodu.

We report the computer control system of simultaneous monitoring of cardiotocography and pulse oximetry during labour. Five of twenty five patients have abnormal CTG results with oxygen saturation over 30%. In the three subjects severe late decelerations were related with decrease of saturation to 20%. Mean SaO2 measurements ranged from 62% at the early stage of labour to 45% in the second stage. We conclude that fetal pulse oximetry plays important complementary role among methods of intrapartum monitoring and it will be used in every case with suspicious or pathological CTGm.