[Histochemical studies on Echinorhynchus gadi (Acanthocephala)]

The distribution of glycogen, polysaccharide, mucopolysaccharide, lipid and nucleic acid has been studied in Echinorhynchus gadi(Acanthocephala). The results were summarized as follows: 1. Glycogen and polysaccharide was demonstrated by Bauer PAS reaction technique and was found in fertilization membrane in ovum, central nuclear mass in acanthor and lemnisci, hypodermis in cystacanth. 2. Mucopolysaccharide was demonstrated by Mowry alcian blue staining technique and was found in outer membrane, fibrillar coat, fertilization membrane and inner membrane in acanthocephalan ova. 3. Lipid was demonstrated by Smith Nile blue stain and Lison Sudan black B staining technique and was found roughly parallel to that of polysaccharide. 4. Nucleic acid was demonstrated by Rosenbeck Feulgen reaction, Taft methylgreen-pyronin stain and Diengdoh acridine orange staining technique and found in central nuclear mass in acanthor, also, was found in lemnisci, proboscis and hypodermis in cystacanth.

[Cytochemical study on lipase and nucleic acid in Trichomonas vaginalis]

Cytochemical demonstrations of lipase and nucleic acid in Trichomonas vaginalis were attempted. Modified Gomori tween method for lipase and acridine orange method for nucleic acid was applied. Trichomonas vaginalis incubated in the isolation mediurn (C.P.L.M.) were pooled and fixed using 0.1 N McIlvaine buffer saline and cold acetone for lipase and Walpole acetate buffer saline and acetic alcohol for nucleic acid. The results were obtained as follows: Activity of lipase and nucleic acid were recognized in the cytoplasm of T. vaginalis as scattered positive granules stained in blue yellow-green bright reddish color respectively. However these reactions were not shown in nucleus, nuclear membrane, undulating membrane etc. Present authos believe the negative finding of acridine orange staining in nucleus should be studied further.

[Studies On Glutamic Pyruvic- And Oxaloacetic Transaminase Of Different Organs Of Ascaris Lumbricoides Suis]

A Study on glutamic pyruvic and oxaloacetic transaminase of different organs(e.g intestine, seminal vesicle, reticular tissue, uterus, ovary, testes) in Ascaris lumbricoides suis have been investigated. The activity of transaminase were determined on the whole homogenates and subcellular fractions separated by differential centrifugation. The activity of glutamic pyruvic and oxaloacetic were assayed by colorimetric method of Reitman-Frankel. The results were obtained as follows: 1. About ninty percent of the glutamic pyruvic and oxaloacetic transaminase in different organs was found to be localized in the supernatant fraction with the separation of differential centrifugation. And it was found that ten percent of glutamic pyruvic and oxaloacetic transaminase exists in the mitochondrial fraction. 2. The specific activity of glutamic oxaloacetic transnaminase in different organs was relatively higher than the glutamic pyruvic transaminase activity.

[Studies On Efficacy And Dosage For Schoolchildren Of Some Anthelminthic Drugs]

1. The evaluation of the effects of anthelmintic drug dose was done by the eggs reduction and the eggs negative conversion rate. 2. The drug dose according to the age of the patient was estimated by the formula of Augsberger, the dose according to the body weight of patient was estimated by the Rule of Six, and the dose to the body surface of the patient was estimated by the formula of Costeff. 3. It is considered that the application of the drug dose according to the age of the patient resulted in reduction of dose in the younger patient, and resulted in the excess dose in older patient. The application of the drug dose according to the body weight also resulted in the reduction of dose in the lighter patient and the excess dose in the heavier patient. 4. It is suggested that the application of drug dose according to the body surface is more reasonable than those by the age and the body weight. The value of this application was also proved to be medium value between those of age method and body weight method.

[Study on the trematode parasites of the birds in Korea]

Authors carried out an investigation of the helminthic parasites of the wild birds in Korea. Most wild birds were collected from Kyoung Sang Do, Kyoung Kee Do and Seoul area from September 1970 to March 1973. A total of 70 wild birds, 19 species were examined, which comprised as follows; 10 Anas platyrhynchus, 10 Anas fulicata, 4 Anas poeciolorhyncha zonorhyncha, 8 Anser albifrone albifrone, 5 Anser cygonopsis cygnoid, 10 Aythya fuligula, 6 Gallicrex cinerea, 2 Porgana fusca, 2 Phisianum calichius korponri, 4 Sterptopilla orientalis, Podiceps cristatus, Larus crasirotatus, Malantta fusca, Garis artcus, Buetes buteo, Egretta alba, Starmus philpiens, Bombycilla granulus. Trematode parasites found from six species of the wild birds. Echinostomatidae has been found from intestine of the Anas platyrhynchus platyrhynchus, Anas poeciolorhyncha zonorhyncha, Anas fulcata. Notocatylus attenuatis found from intestine of the Podiceps cristatus, Anas fulcata. Catylurus japonicus was found from rectum of the Anas platyrhynchus platyrhynchus, Anas poeciolorhyncha zonorhyncha.

[A Case Report Of Human Thelaziasis]

Authors were observing a case of infection with Thelazia callipaeda in Korean girl, on 11th Sep. in 1971. The case was 23 years old Korean girl who visited with the complaints of foreign body sensation and lacrimation in right eye. The worm had slender creamy white which was fixed in 10 percent formaline solution. The worm is 10.5mm in length, 0.5mm in body diameter. The oral end in provided with a chitinoid capsule. Esophagus moderately short, tail blunt, poserior extremity had a distinctly recurved. Spicules dissimilar, left spicule is 0.1mm in length, and right spicule is 0.08 mm in length.

[A case report of intestinal eosinophilic granuloma du to Ascaris Ova]

This is a case report of intestinal eosinophilic granuloma caused by Ascaris ova and worm which is supposed to be rare in Korea. CASE: A 23 year old healthy female reached Pusan Sanitation Hospital with complaints of high fever and abdominal pain on December 3 in 1966. Examination: Her temperature was 99.6'F. Pulse 80. Abdominal palpation showed muscle rigidity and tenderness. On the right side of the abdomen diagnosis due to ruptured appendix was made, and a laparotomy was performed the same day. OPERATION: Intestinal perforation by a Ascaris worm in the caecum about 7 cm from the ileo-caecal junction was also found. The worm was liquefied already. The intestine was edematous. Numerous rice sized nodules were seen on the intestine. The omentum was markdly inflammed and was adhered with a fist size mass. The mass and appendix were also resected in order to do a histological study. PATHOLOGY: Two kinds of tissues were examined : one a mesenteric mass, the other lymph node. MICROSCOPICALLY: it showed intensive and entirely necrotic tissue in which numerous parasitic ova were surrounded by granulomatous inflammatory cells with eosinophiles. The parasitic ova were degenerative and partialy necrotic although they had three layers of egg shell which are identified with Ascaris ova.

[Histochemical Study On Trematodes: Distribution Of Carbonic Anhydrase Activity]

The purpose of the present study is to demonstrate the distribution of carbonic anhydrase pattern in the various termatodes (Fasciola gigantica, Paramphistoma orthocoelium, Paragonimus westermani) by means of Kurada staining method, and to correlate these findings with the histochemical data and harboring location. The results are summarized as follows: 1. In Fasciloa gigantica, carbonic anhydrase activity was positive in the vitelline gland cells and eggs in the uterus. 2. In Paramphistoma orthocoelium, carbonic anhydrase activity was positive in the vitelline gland cells and eggs in the uterus. 3. In Paragonimus westermani, carbonic anhydrase activity was positive in intestinal mucous membrane, vitelline gland cells and eggs.

[Studies On Amphistoma In Korean Cattle]

The present studies were done to determine the prevalence of Paramphistoma in Korean cattle. Material have been collected by authors at the slaughter house of Pusan in Korea from February to July of 1966. The fixing solution used were 10 percent formaline, Carnoy-solution, Zinker and Bouin solution for total worm body preparation. The staining was done with acetocarmine and Delafild's Hematoxylin for total preparation and double Hematoxylin-Eosin staining for microscopical section slides. The following results were obtained: The authors have identified six species of Amphistoma from Korean cattle, and these are Paramphistoma orthocoelium, P. cervi, P. explanatum, P. gotoi, Gastrothylax elongatus and Gastrothylax cobblodi. The prevalence status is as follow: Paramphistoma orthoceolium and Gastrothylax elongatus are very common and Paramphistoma cervi, Paramphistoma explanatum are rather common, but Paramphistoma gotoi and Gastrothylax cobblodi are very rare in Korean cattle.

[Taxonomical study on the Fascioloidae in Korea]

In order to study species of the Fascioloidae in Korea, a series of morphological and taxonomical studies were carried out and the following results were obtained. 1)Among the species of the Fascioloidae in Pusan area, intermediate form (F. indica?) were 70 %, F. gigantica 25 % and F. hepatica, 5 %. 2)None experimental, natural infective rate of the Fasciolosis was 3.3~3.4 % among the cattles (Bos taurus var. domesticus) in Pusan area.

[Epidemiological Studies On The Acaroid Mite]

Epidemiological study on Acaroid mite in dust different sites were carried out and the following results were obtained. 1. Tyrophagus dimidiatus, Chibidania tokyoensis and Ornithonyssus nagayoi were found in living room(46.2 %), bath room(36.7 %), garden(27.0 %), shopping-store(28.8 %) and ware-house (29.2 %) in the Pusan area. 2. Dermanyssus gallinae and Rhizoglyphus echinopus were found mainly in class rooms of girl's high school(34.0 %) and class room of girl's middle school(40.0 %) as well as in primary school(13.2 %), but they are not found in class rooms of boys high school. 3. Generally, the various mites were found in the living room(57.0 %), under the carpet(50 %), under the furniture(52.0 %), on the furniture(27.5 %), lavatory(35.7 %) and garden(23.8 %).