RESUMO
It remains a challenge to develop cost-effective, high-performance oxygen electrocatalysts for rechargeable metal-air batteries. Herein, zinc-mediated zeolitic imidazolate frameworks are exploited as the template and nitrogen and carbon sources, onto which is deposited a Fe3 O4 layer by plasma-enhanced atomic layer deposition. Controlled pyrolysis at 1000 °C leads to the formation of high density of Fe3 O4- x few-atom clusters with abundant oxygen vacancies deposited on an N-doped graphitic carbon framework. The resulting nanocomposite (Fe3 O4- x /NC-1000) exhibits a markedly enhanced electrocatalytic performance toward oxygen reduction reaction in alkaline media, with a remarkable half-wave potential of +0.930 V versus reversible hydrogen electrode, long-term stability, and strong tolerance against methanol poisoning, in comparison to samples prepared at other temperatures and even commercial Pt/C. Notably, with Fe3 O4- x /NC-1000 as the cathode catalyst, a zinc-air battery delivers a high power density of 158 mW cm-2 and excellent durability at 5 mA cm-2 with stable 2000 charge-discharge cycles over 600 h. This is ascribed to the ready accessibility of the Fe3 O4- x catalytic active sites, and enhanced electrical conductivity, oxygen adsorption, and electron-transfer kinetics by surface oxygen vacancies. Further contributions may arise from the highly conductive and stable N-doped graphitic carbon frameworks.
RESUMO
BACKGROUND: Bacterial wilt caused by Ralstonia solanacearum species complex is an important soil-borne disease worldwide that affects more than 450 plant species, including peanut, leading to great yield and quality losses. However, there are no effective measures to control bacterial wilt. The reason is the lack of research on the pathogenic mechanism of bacterial wilt. RESULTS: Here, we report the complete genome of a toxic Ralstonia solanacearum species complex strain, Rs-P.362200, a peanut pathogen, with a total genome size of 5.86 Mb, encoding 5056 genes and the average G + C content of 67%. Among the coding genes, 75 type III effector proteins and 12 pseudogenes were predicted. Phylogenetic analysis of 41 strains including Rs-P.362200 shows that genetic distance mainly depended on geographic origins then phylotypes and host species, which associated with the complexity of the strain. The distribution and numbers of effectors and other virulence factors changed among different strains. Comparative genomic analysis showed that 29 families of 113 genes were unique to this strain compared with the other four pathogenic strains. Through the analysis of specific genes, two homologous genes (gene ID: 2_657 and 3_83), encoding virulence protein (such as RipP1) may be associated with the host range of the Rs-P.362200 strain. It was found that the bacteria contained 30 pathogenicity islands and 6 prophages containing 378 genes, 7 effectors and 363 genes, 8 effectors, respectively, which may be related to the mechanism of horizontal gene transfer and pathogenicity evaluation. Although the hosts of HA4-1 and Rs-P.362200 strains are the same, they have specific genes to their own genomes. The number of genomic islands and prophages in HA4-1 genome is more than that in Rs-P.36220, indicating a rapid change of the bacterial wilt pathogens. CONCLUSION: The complete genome sequence analysis of peanut bacterial wilt pathogen enhanced the information of R. solanacearum genome. This research lays a theoretical foundation for future research on the interaction between Ralstonia solanacearum and peanut.
