The effect of gel "Xeliogel" at the stages of the regeneration of aseptic burn wound in the experiment.

Due to the problem of treating some types of burns, it is necessary to develop new drugs. For this purpose, pharmacological studies of developed gel "Xeliogel" (based on biological material with regenerating action), which accelerates the healing of superficial burns, have been developed and previously carried out. AIM: The aim of this work was to establish the histological changes of the burn wound in the dynamics and after the experimental thermal injury and in the conditions of application of the gel "Xeliogel". MATERIALS AND METHODS: The experiments were performed on mature Wistar rats of both sexes weighing 250-260 g, randomly divided equally into four groups: 1 - group of intact animals; 2 - control pathology group; 3 - group for the treatment of which used the developed gel "Xeliogel" and 4 - group of animals with the comparison drug "Solcoseryl" (Legacy Pharmaceuticals Switzerland GmbH, Switzerland). Histological indicators of the effect of gels were recorded 3 times: on the 3rd (stage of burn shock), 7th (stage of early toxemia) and 14th (stage of late toxemia) days of the experiment. Examination of micropreparations was performed on a Nicon Eclipse CI-E microscope. Microscopy of microscopic images was performed using a Sigeta M3CMOS 14000 camcorder and Toup View software on a personal computer. RESULTS: During using the developed gel "Xeliogel" it is established that on the 3rd day of the experiment the wound surface is covered with a crust, which is formed by plasma proteins and with destroyed elements of blood. On day 7 after the experimental thermal injury, both "Xeliogel" gel and "Solcoseryl" gel were found to show that the skin defect area was also covered with a film, the main components of which were destroyed blood cells and fibrinous mass. When examining the area of the defect on the 14th day of the experiment with the use of the comparison drug "Solcoseryl" gel, wounds healing covered with an epithelial layer with a clear-layered structure was observed. CONCLUSIONS: The histological evaluation of the use of "Xeliogel" gel established that the developed gel provides healing of the wound defect on the 14th day of the experiment. There is a well-defined marginal regeneration of the epidermis, the formation of the basement membrane, the restoration of the papillary layer of the dermis and capillary system.

Therapeutic efficacy of the developed gel "Xeliogel" on a burn wound model in rats.

Among the high-tech methods of wound therapy, the leading place belongs to the technology of manufacture and use of cryolyophilized tissues and bioorganic drugs. Crushed xenoderm's powder stimulates the regenerative processes of the skin and improves the healing process of wounds, as it is enriched with macro- and microelements, amino acids and other cellular elements necessary for skin regeneration. AIM: The aim of this work was to study the wound-healing effectiveness of the developed gel "Xeliogel" on the animal model of aseptic superficial burns. MATERIALS AND METHODS: Evaluation of the degree of catabolism and membrane-destructive processes was performed by determining the marker enzymes of cytolysis - aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum, total protein and middle molecular mass in animals with experimental thermal burns. The developed gel "Xeliogel" was applied to the animals for therapeutic purposes. "Xeliogel" is based on aqueous extraction of xenoderm and with lidocaine hydrochloride. In the second group for comparison the Solcoseryl was applied in the form of a gel (Switzerland). This medicine belongs to the group containing active pharmaceutical ingredients of natural origin, namely protein-free dialysate from the blood of calves, and is intended for the treatment of burns of I-II degrees. RESULTS: When using the developed gel "Xeliogel" in experimental thermal burns, normalization of total protein was observed on 14-th days, which is on 7 days faster than under burn conditions, decrease the levels of ALT and AST in the serum with a return to a normal on a 7 day, relative to the control pathology group. In the groups of animals, on whose burn wounds "Xeliogel" gel was applied, already on the 14-th day endogenous intoxication was significantly reduced by 29 % compared with the control group of animals. During the experiment to reduce the area of the wound surface, it was determined that the developed gel "Xeliogel" showed a pharmacological effect similar to the comparison drug "Solkoseryl" gel. CONCLUSIONS: On the model of aseptic burn injury in rats, was determined that the developed gel provides a significant reduction in necrotic processes, the level of endogenous intoxication against the background of restoring the level of total protein in the circulating blood. The results of the research confirm the effectiveness of using the developed gel "Xeliogel" for local treatment of burns of I-II degrees, as the duration of treatment of burns reduced by 4 days compared to the control group and the developed gel is similar to the comparison drug.

Characteristics of structured medical hemostatic sponges as a medical devices for stop bleeding and for close the wound.

Medical sponge is a tool that has absorbent and antiseptic properties, as well as stimulates tissue regeneration and is used in surgery, neurosurgery, dentistry, otolaryngology and gynecology to stop blood loss or to close wound surfaces (burns, trophic ulcers). To summarize the information of the professional literature on medical (hemostatic) sponges, their classification and applications, the areas of their use in medical practice and also modern excipients and active pharmaceutical ingredients that can be used in their technology. AIM: The aim of the study was to summarize data on the characteristics, classification of medical sponges, auxiliary substances and active pharmaceutical ingredients used in the technology of these agents and medical use. MATERIALS AND METHODS: Methods of information retrieval, analysis of statistics and scientific literature on medical sponges and prospects of their use were applied in the study. RESULTS: Despite the creation and research of a large number of medical sponges based on gelatin, chitosan, cellulose derivatives, collagen and other excipients, as well as the clinical demonstration of their efficacy and need for use, medical sponges are still need refinement and further study. Because of it, the search for the perfect foundation to create universal medical sponge is still ongoing. CONCLUSIONS: Expanding the range of medical sponges and researching new excipients for their manufacture are a promising area of modern medicine and pharmacy.