RESUMO
RAPD-analysis was used to estimate intraspecies-specific variability in malaria mosquitoes, the major vectors of malaria in Central Asia. The high heterogeneity of RAPD-loci was found in 7 populations of An. superpictus and 2 populations of An. pulcherrimus. Genetic distances between the populations were determined on the basis of analysis of the frequencies of 15 RAPD-loci. There was a genetic similarity of An. superpictus populations in the malaria foci of Tadjikistan with those in the neighboring Surkhandariya Region of Uzbekistan.
Assuntos
Anopheles/classificação , Insetos Vetores/classificação , Malária/parasitologia , Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos , Animais , Anopheles/genética , Anopheles/crescimento & desenvolvimento , Primers do DNA , Genes de Insetos/genética , Insetos Vetores/genética , Insetos Vetores/crescimento & desenvolvimento , Larva/genética , Malária/prevenção & controle , Especificidade da Espécie , Tadjiquistão , UzbequistãoRESUMO
Diploid gynogenetic progenies were obtained in the stellate sturgeon Acipenser stellatus, Russian sturgeon A. gueldenstaedtii, and sterlet A. ruthenus by means of insemination of the eggs with UV-irradiated spermatozoa and suppression of the second meiotic division by heat shock. The gynogenetic nature of experimental fish was confirmed by RAPD-PCR analysis of DNA. Effective photoreactivation of UV-induced lesions of spermatozoa was shown in the case of illumination of the fertilized eggs with visible light. This phenomenon should be taken into account when determining the doses of irradiation that allow inactivation of the male chromosomes and incubating gynogenetic embryos. Gynogenetic stellate and Russian sturgeons are viable and can be reared in order to study the mechanism of sex determination in sturgeons.
Assuntos
Peixes/genética , Meiose , Partenogênese/genética , Animais , Cromossomos/efeitos da radiação , Diploide , Relação Dose-Resposta à Radiação , Embrião não Mamífero , Feminino , Peixes/embriologia , Resposta ao Choque Térmico , Inseminação Artificial/métodos , Masculino , Técnica de Amplificação ao Acaso de DNA Polimórfico , Processos de Determinação Sexual , Espermatozoides/efeitos da radiação , Raios UltravioletaRESUMO
We analyzed the possibility of triplex formation in the human alpha 1-antitrypsin gene. Conditions and nucleotide sequence dependence of triplex formation and thermostability of the product were determined.