[Assesment of the nutrition and antioxidant activity of biological liquids in students].

Studying of quality of nutrition of students is of great importance as it is one of the major factors defining the state of health, especially during the periods of adaptation to the changing conditions of the environment and stressful situations. Stability or, on the contrary, predisposition to stressor violations is defined by numerous factors including the nutritive status of an organism. The purpose of the research was the analysis of the actual diet of the studying youth during educational process and assessment antioxidant activity of biological liquids of an organism. 74 students shared in a research: 30 young men and 44 girls. For assessment of the actual nutrition a method of the food diary within 5 days, excepting days off and holidays, has been used. It has been established that frequency of a meal at most of students was 3-4 times a day. Most of students consumed the first dishes1-2 times a week (56.7% of young men and 63.6% of girls), at the same time these dishes were absent in a diet at 13.6% of girls. The second dishes were present in the diet of all students. In both groups the average index of body weight was in limits of norm (18.5-25.0 kg/m2). At the same time body weight was reduced at 6.7% of young men and 11.4% of girls; excess body weight was revealed at 20.0% of young men and 6.8% of girls. 73.3% of young men and 81.8% of girls had normal body weight. In young men the calorie content of the diet, the protein and fat content slightly differed from the norm. At the same time, a carbohydrate imbalance was observed - an increase in the proportion of simple carbohydrates (sugars) (p≤0.05) and a decrease in the content of starch and dietary fiber (p≤0.05). In girls, protein intake was below the normal level and a similar carbohydrate imbalance was noted (p≤0.05). The content of vitamins in the diet of both boys and girls was reduced. Youngsters had a deficiency of ß-carotene and vitamin C, in girls it was aggravated with vitamins B1 and B2 (p≤0.05). In the diet of the surveyed, a deficiency of such minerals as calcium and magnesium was noted; in girls - potassium, phosphorus and iron in addition (p≤0.05). Excessive element in the diet was sodium. In the diet of young men the content of phosphorus and iron exceeded the norm. Thus, the analysis of the diet of students indicated a lack and imbalance in the content of both macroand micronutrients, which was most pronounced in girls. The total antioxidant activity of biological liquids of an organism - blood serum, urine and saliva (determined by amperometric method using a system for liquid chromatography) decreased among: urine, blood serum, saliva. Blood serum of young men possessed greater antioxidant activity than in girls; while in urine the dependence was reversed. Thus, in order to increase the antioxidant status of the organism during periods of adaptation and stress, a qualitative and quantitative balance of the diet and increased consumption of antioxidants is necessary.

DETOXIFICATION AND DESENSITIZATION OF THE BODY IN THE TRADITIONAL MEDICINE / Монголын эм зүй, эм судлал

The present work presents information on the development and use of technologies for detoxification and desensitization of the body based on the tenets of the traditional medicine.KEY WORDS: traditional medicine, purification of the body, development and use, technology for detoxification and desensitization.INTRODUCTIONIn recent decades it has been noted the decrease in the index health as well as the incidence rate growth due to the deterioration of environmental conditions, the increased fastness of living, and the reduction of social protectability [7]. Pollution of the inner medium of the body with substances received through water, food and air causes a serious worry. These substances include plant and animal growth stimulants, fertilizers, pesticides, various coloring matters for foodstuffs, preservatives, drugs, salts of heavy metals, radionuclides, etc. Intensive xenobiotic contamination gives rise to dangerous acute intoxications and the development of chronic intoxication of the body followed by the disturbances of inner organs functioning resulting in the decrease of the bodily resistance and growth of incidence rate [2, 3]. Growing environmental and social pressing leads to the impairment of detoxification, desensitization, immune and other adaptive mechanisms of the human body [4, 5] that is a cause of many diseases. According to the data of World Health Organization (WHO) there is a tendency to the development of severe chronic cases due to intoxication and sensitization of the body [7]. Under the circumstances when the rate of chronic cases increases in number and the future generations are threatened with chronic diseases, the detoxification of the body seems of great importance.THE AIM of the given work is to develop and introduce into practice the detoxification and desensitization technology based on the traditional medicine.THE OBJECTS for studies were plant remedies stimulating detoxification and drainage function of the hepatobiliary system, gastro- intestinal tract, kidneys and skin created on the base of the traditional medicine formulae (nor bu dun thang, shi rguid, nephrophyt, sudorific plant collection). The studies were carried out with the use of pharmacological, clinical, biochemical and statistical methods [1, 6].RESULT. The lymphatic system is known to be the main drainage system excreting endo- and exotoxins from extracellular space, from cells and tissues [2, 3]. The system is capable to transport even big structures (high-molecular proteins, toxins) which enter the system through valves but not through semi-impermeable vessel walls as in the case of circulatory system. As a result transported toxins enter the venous duct. Further, the processes of detoxification take place mainly in the liver and kidneys. To avoid the “overloading” of these organs it is necessary to increase their functional activity using relevant medicinal preparations [4, 5, 7]. The increase in functioning of the given organs and drainage are regarded as the first level of detoxification and desensitization of the body. At present, taking into account the chemization of the home, water, air and food pollution and uncontrolled intake of medicinal preparations, it is necessary to pay attention to the “medicine of evacuation” i.e. to the purification of inner medium of the body [1, 5]. The technology for detoxification and desensitization of the body developed on the base of the traditional medicine, suggests the stage-by-stage intake of the remedy stimulating hepatobiliary system (nor bu dun thang) for 9 days; the remedy promoting the function of gastro-intestinal tract (shi brguid) for 9 days; the remedy stimulating the function of kidneys(nephrophyt) for 9 days and sudorific remedy (sudorific plant collection) for 9 days. Pre-clinical studies which were carried out on white male Wistar rats have revealed drainage and detoxification influence of the given remedies. The combined administration of the above remedies to SBA mice has shown that they have desensitization effect. Clinical studies of the given technology were carried out on the patients with endogenic intoxication and pollution of the inner medium of the body, particularly, on the persons suffered from atopic dermatitis and eczema. The Table 1 shows the results of therapy with the use of the developed technology of detoxification and desensitization of the body. The clinical studies were carried out on the patients suffered from allergic dermatoses (atopic dermatitis, eczema) at the Buryat Republican dermato-venerologic dispensary.The data given in the Table 1 show that the use of the given technology of detoxification and desensitization of the body improves the general state of the majority of patients followed by normalization of biochemical indices in the blood serum. Biochemical investigations performed after treatment have shown the decrease of the malonic dialdehyde (MDA) content and circulating immune complexes (CIC); the transaminase activity and concentration of α and α2 –globulins were also decreased as compared with the data obtained before treatment. The same tendency was observed in patients with the subacute stage of atopic dermatitis. Only in 5 patients no significant effect was noted. When the given technology was used for the treatment of eczema no significant positive changes in biochemical indices of the blood serum were noted in 32 cases in spite of satisfactory general state of the patients on the background of the treatment.The studies conducted have revealed the tendency to the improvement of the general state of the patients and the improvement of biochemical indices in the blood serum at the final terms of the treatment with the use of the given technology. Nevertheless in some cases a full health restoration was not noted in a number of patients, especially in patients with eczema. It is indicative of probable recurrence of the disease. Refresher course of the treatment is advisable.

[The effect of ultrasound, the polyene antibiotic amphotericin B and their combination on the biomembranes of yeast-like fungi in the genus Candida]. / Vliianie ul'trazvuka, polienovogo antibiotika amfoteritsina B i ikh sochetaniia na biomembrany drozhzhepodobnykh grinov roda Candida.

The effect of ultrasound (US) and the antibiotic amphotericin (A-B) on the structure of Candida albicans membranes. The most expression of destructive processes and appearance of free radicals in mycetic cells was observed in combination of US and A-B by chemiluminescence and ultrastructural analyses. The mechanisms of action of US and A-B on mycetic cells were discussed in the article. The expedience of the combination of US and A-B in candidiasis as a factor, increasing fungicidal effect on the candidiasis agent of Candida albicans.

[State of the free-radical oxidation system in normobaric hypoxia]. / Sostoianie sistemy svobodnoradikalnogo okisleniia pri deistvii normobaricheskoi gipoksii.

The experiments on the rats have revealed that 7-hour action of 10% hypoxic gas mixture (HGM-10) exerts no effect on the parameters of Fe(2+)-induced chemiluminescence and rate of accumulation of TBA-active products in the heart, liver, kidney, brain tissues and blood plasma. Two-week adaptation to intermittent effect of HGM-10 causes some activation of free-radical oxidation recorded in blood plasma and the more pronounced increase in power of the endogenic antioxidant system. It is assumed that the revealed changes in the state of the homeostatic system of free-radical oxidation and antiradical protection of the organism are of importance in the mechanism of the known preventive and curative action of intermittent normobaric hypoxia.

[The effect of antioxidants on the fast burst of Fe2(+)-induced chemiluminescence]. / Vliianie antioksidantov na bystruiu vspyshku Fe2()-indutsirovannoi khemiliuminestsentsii.

A new micromethod for measuring the intensity of free radical oxidation reactions, based on an analysis of "fast flash" of iron-induced chemiluminescence parameters has been designed. The optimal concentration parameters of the reaction system have been determined. The effects of the antioxidants mexidol, probucol sodium dibunat and fenoxan have been examined in vitro.

[Effects of extreme factors and antioxidant alpha-tocopherol on the intensity of blood plasma chemiluminescence]. / Vliianie ékstremal'nykh vozdeistvii i antioksidanta alfa-tokoferola na intensivnost' khemiliuminestsentsii plazmy krovi.

It was established in experiments on rats that intensity of "fast flash" of iron-induced chemiluminescence of biological samples containing microvolumes of blood plasma correlate with resistance of organism to severe hypoxia. The new method is promising for the estimation of the sensitivity of intact organism to severe hypoxia, for registration of antioxidant therapy effects and for studying the mechanisms of extreme factors influence on the organism.