The relationship of p63 expression with cell proliferation and apoptosis in DMBA-induced hamster buccal pouch carcinogenesis

PURPOSE: Abnormalities in the p53 gene are regarded as the most consistent genetic abnormalities detected in head and neck squamous cell carcinogenesis. Two new members of the p53 gene family, p73 and p63 have recently been identified. They share considerable sequence homology with p53 in the transactivation, DNA binding, and oligomerization domains, indicating possible involvement in carcinogenesis. Disruption of the homeostatic balance between proliferation and apoptosis is widely believed to contribute to human oral carcinogenesis. The aim of this study was to analyze expression of p63 in squamous cell carcinogenesis and to compare with immunochemical markers representing cell proliferation and apoptosis. MATERIALS AND METHODS: Using the Syrian hamster oral cancer model, the fraction of apoptotic (apoptotic index-AI), proliferating (mitotic index-MI) and p63 expressing keratinocytes were examined at normal, dysplastic and malignant oral epithelium using the TUNEL assay, PCNA and p63 immunostaining. RESULTS: p63 significantly increased between normal and dysplastic epithelium and between dysplastic and malignant epithelium. PCNA significantly increased between normal and dysplastic epithelium and between normal and malignant epithelium. However, increase between dysplastic and malignant epithelium, though still increasing, was not statistically significant. The percentage of TUNEL positive cells increased from normal to dysplastic epithelium and returned to normal keratinocyte level in the malignant epithelium. However, differences between tissue types were not significant. The ratio of MI:AI increased significantly only in the dysplastic-malignant epithelial transition. The increase of p63 expression closely reflected the change in the MI:AI ratio during oral carcinogenesis. CONCLUSION: The p63 may be associated with the regulation of epithelial proliferation and apoptosis in DMBA-induced hamster buccal pouch squamous cell carcinogenesis. Further study is required to investigate which p63 isoforms are involved in hamster buccal pouch carcinogenesis.

Blepharoplasty by various causes

Recently, the number of facial contouring surgery such as angle reduction or malarplasty in Oral and maxillofacial surgery is increasing steadly as the demand for maxillofacial esthetic surgery of the patients is growing. Looking around international trend of oral and maxillofacial surgery, nowadays workshop or symposium about facial soft esthetic surgery such as corrective rhinoplasty, blepharoplasty, face lift are held. This means that maxillofacial esthetic surgery is new scope which oral and maxillofacial surgeons should develope. Therefore the authors present cases of blepharoplasty which got hardly used to oral and maxillofaical surgeon with literature review in order to raise the concern about maxillofacial esthetic surgery.

Nasal augmentation using goretex