Phyllodes tumor of the breast with actin inclusions in stromal cells: diagnosis by fine-needle aspiration cytology.

The occurrence of hyaline inclusions in stromal cells in fibroepithelial tumors of the breast is very uncommon. These inclusions, characteristic of infantile digital fibromatosis, are comprised of actin filaments. This report illustrates a case of a benign phyllodes tumor of the breast with inclusion bodies, identified by fine-needle aspiration. Histologically, many of the stromal cells contained round intracytoplasmic inclusions, with positivity for smooth muscle actin.

Phyllodes tumor in ectopic breast tissue of the vulva.

Phyllodes tumor arising from ectopic breast tissue of the vulva is an extremely rare occurrence. A case is reported in a 34-year-old nulligravida woman, presenting as a nodule on the left labium majus. A simple excision was carried out, and no recurrence has been detected after 18 months of follow-up.

Microadenoma hipofisario secretor de TSH: estudio morfológico tras tratamiento con lanreótido / TSH secreting pituitary microadenoma: Morphological study following treatment with lanreotide

Los adenomas hipofisarios secretores de TSH son muy infrecuentes. Los pacientes presentan concentraciones elevadas de TSH, clínica de hipertiroidismo y tumor hipofisario, habitualmente un macroadenoma. El presente trabajo describe el caso de un microadenoma hipofisario secretor de TSH tratado con análogos de somatostatina (lanreótido) y posteriormente con cirugía transesfenoidal. El estudio patológico confirmó un adenoma hipofisario con positividad inmunohistoquímica para TSH y subunidad alfa. Un hallazgo relevante fue la presencia de abundante fibrosis tumoral. La fibrosis es un fenómeno frecuente en los tirotropinomas, por lo que resulta difícil establecer su relación con el tratamiento con lanreótido (AU)

Leiomiosarcoma infantil con células gigantes de tipo osteoclástico / Infantile leiomyosarcoma with osteoclast-like giant cells

El presente artículo describe el caso de un paciente de siete años intervenido por tumoración de 3 cm en la pared abdominal. El estudio anatomopatológico demostró un leiomiosarcoma de grado bajo con células gigantes de tipo osteoclástico. La presencia de células gigantes de tipo osteoclástico en el leiomiosarcoma ha sido excepcionalmente descrita, lo que unido a la rareza de eta neoplasia en la edad infnatil puede plantear problemas de diagnóstico diferencial con otros tumores de partes blandas infantiles benignos o malignos, especialmente con la miofibromatosis infantil. La hibridación in situ no demostró que el virus de Epstein-Barr estuviera implicado en la patogénesis de este caso (AU)

Autoantibodies against P53 protein in patients with transitional cell carcinoma of the bladder.

Cellular accumulation and conformational changes of mutant p53 could act as immunogens for auto-antibodies (auto-Abs) generation when altered p53 from tumoral cells reaches the blood stream. Our main objective was to compare the presence and clinical implications of p53-antibodies in serum with the immunohistochemical (IHC) tissue overexpression of protein p53 in patients with transitional cell carcinoma of the bladder, evaluating their association with bladder cancer parameters and their prognostic value. The study comprised 59 patients with bladder cancer (group 1) and 15 healthy controls (group 2). Serum p53-Abs were measured by ELISA. Mutant p53 protein IHC overexpression was examined from paraffin embedded tissues using monoclonal DO-7 Ab. Serum p53-Abs were detected in 14/59 and IHC P53 was positive in 24/59 patients from group 1. All p53-Abs positive patients had IHC p53 positive tumors, but some patients with IHC positive immunoreactivity showed undetectable p53-Abs. None of the healthy controls had detectable p53-Abs. Titres of p53-Abs were associated with stage and grade. P53 overexpression was dependent on stage, grade, pattern of growth and focality. P53 Abs showed a significant prognostic value for disease free survival (p = 0.0059) and life expectancy (p < 0.0005) and for IHQ p53 for life expectancy (p = 0.0033). Patients with positive P53 Abs showed a higher probability for a shorter survival OR = 6.38 (1.77-22.99) than those who were positive for IHQ p53 OR = 4.00 (1.31-12.8) or those who were negative for p53 Abs and/or IHQ p53. The measurement of p53 Abs in serum appeared to be a simple determination which might reflect the p53 status and might help in the selection of those bladder cancer patients with a worse prognosis.