Note: Calibration of EBT3 radiochromic film for measuring solar ultraviolet radiation.

Solar (UVA + UVB) exposure was assessed using the Gafchromic EBT3 film. The coloration change was represented by the net reflective optical density (Net ROD). Through calibrations against a UV-tube lamp, operational relationships were obtained between Net ROD and the (UVA + UVB) exposures (in J cm(-2) or J m(-2)). The useful range was from ∼0.2 to ∼30 J cm(-2). The uniformity of UV irradiation was crucial for an accurate calibration. For solar exposures ranging from 2 to 11 J cm(-2), the predicted Net ROD agreed with the recorded values within 9%, while the predicted exposures agreed with the recorded values within 15%.

A 3-class asynchronous BCI controlling a simulated mobile robot.

We present our design and online experiments of a 3-class asynchronous BCI controlling a simulated robot in an indoor environment. Two characteristics of our design have efficiently decreased the false positive rate during the NC (No Control) mode. First, three one-vs-rest LDA classifiers are combined to control the switching between NC and IC (In Control) mode. Second, the hierarchical structure of our controller allows the most reliable class (mental task) in a specific subject to play a dominant role in the robot control. A group of simple rules triggered by local sensor signals are designed for safety and obstacle avoidance in the NC mode. In online experiments, subjects successfully controlled the robot to circumnavigate obstacles and reach small targets in separate rooms.

Treadmill exercise increases cell proliferation in hippocampal dentate gyrus in alcohol-intoxicated rats.

AIM: Effect of treadmill exercise on hippocampal cell proliferation under normal conditions has been well documented; however, this effect under alcohol intoxication conditions is not clarified, yet. In the present study, the effect of treadmill exercise on cell proliferation in the dentate gyrus in alcohol-intoxicated rats was investigated. EXPERIMENTAL DESIGN: comparative investigation on number of 5-bromo-2'-deoxyuridine (BrdU)-positive cells in the dentate gyrus 8 days after commencement. SETTING: animal laboratory. PARTICIPANTS: male Sprague-Dawley rats of 5 weeks in age weighing 150+/-10 g. INTERVENTION: animals were divided into 4 groups: the control-rest group, the control-exercise group, the alcohol-treated-rest group, and the alcohol-treated-exercise group. Animals of the alcohol-treated groups were injected intraperitoneally with alcohol (2 g/kg) once a day for 3 days. All animals were injected BrdU (50 mg/kg) intraperitoneally, and rats of exercise groups were made to run on treadmill for 30 min each day for 5 days following alcohol administration. MEASURES: mean number of BrdU-positive cells in dentate gyrus was observed via immunohistochemistry. RESULTS: Treadmill exercise significantly increased the number of BrdU-positive cells in the dentate gyrus. Also, treatment with alcohol for 3 days inhibited cell proliferation and treadmill exercise alleviated alcohol-induced inhibition of new cell formation. CONCLUSION: These results suggest the possibility that treadmill exercise may help in improvement following alcohol-induced brain damage.

Screening and selection of acid and bile resistant bifidobacteria.

Human fecal samples were used as a source of Bifidobacterium strains which are resistant to both acid and bile. The procedure used for screening was as follows: enrichment of Bifidobacterium strains with Bifidobacterium-selective transgalacto-oligosaccharide-propionate (TP) medium followed by acid (pH 2.0) and bile salt stressing (1.5% bile salt (w/v)). Two selected Bifidobacterium strains, designated HJ 30 and SI 31, showed considerably higher rates of survival when incubated in 50 mM phosphate buffer solution adjusted to pH 2.0 or 3.0 or in 50 mM phosphate buffer (pH 7.0) containing 0.5 or 1.0% (w/v) bile salt. HJ 30 and SI 31 were the only strains to have significant growth in Man Rogosa Sharpe (MRS) medium at 0.15% bile salt. All strains tested had similar growth rates in the absence of bile or at an initial pH value of 5.0 or 7.0 as determined by optical density measurements. For SI 31 the number of viable cell counts remained high (6 x 10(7) cfu/ml) for up to 72 h when grown in the skim milk medium, whereas all other strains examined declined to below 10(5) cfu/ml. These results demonstrate that the screening procedures developed in this study are effective for the selection of acid and bile resistant Bifidobacterium strains.