Characterization of scarred vocal fold regeneration after the intervention of extracellular matrix and human amniotic epithelial cells transplanting / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To investigate the treatment for scarred vocal folds by transplanting human amniotic epithelial cells (hAECs)and injecting collagenase as well as hyaluronic acid (HA) for the intervention of the extracellular matrix(EMC), to observe the growth, distribution of hAECs and to assess the abilities of them for scarred vocal fold regeneration.</p><p><b>METHODS</b>The lamina propria was injured by localized resection in thirty-eight vocal folds of twenty rabbits. hAECs were isolated from human amnion and marked by Lenti-GFP. After the formation of vocal fold scarring, hAECs were transplanted into ten vocal folds, collagenase and HA were injected into ten vocal folds, all three were injected into ten vocal folds, none were injected into eight vocal folds, and two normal vocal folds were used as control. At 1 month and 2 months after the transplanting, the survival, the distribution and the cytoactive of hAECs were examined by immunofluorescence method. Meanwhile, at 1 month, 2 months, 3 months and 6 months after the operation, HE staining was performed for histopathological research, Masson trichrome staining and immunohistochemical staining were used for collagen and fibronectin respectively.</p><p><b>RESULTS</b>After implanted into the scarred vocal folds, hAECs could survive in vocal fold lamina propria for two months. The immunofluorescence analysis showed the cytoactive of hAECs.Six months postoperatively, compared with that in the normal vocal folds, collagen in the untreated scarred vocal folds more increased and disorderly distributed; the changes in other three groups were between the two groups above, but the group injected with all of hAECs, collagenase and HA was better than other two groups. Besides, the mean density of fibronectin in the scarred untreated control group was more significantly increased than that in the normal vocal folds; the changes in other three groups were between the two groups above, but the group injected with all of hAECs, collagenase and HA was better than other two groups.</p><p><b>CONCLUSION</b>The transplanting of hAECs and the interventions of EMC by injecting collagenase as well as HA have better abilities in rabbit scarred vocal fold reparation and regeneration by promoting ECM secretion, rational distribution and part ordering arrangement.</p>

Analysis of island flaps of distal pedicled sural nerve accompanying blood vascular for 21 cases of lower extremity soft tissue defects reconstruction / 中国基层医药

Objective To investigate the characterristics of distally based sural flap for the leg reconstruction.Methods Twenty-one patients with soft tissue defects in the instep,heel,ankle and distall one third anterior tibial region were analyzed.Results All flaps survived and healed with the affected area.Conclusion Distally based sural flap was reliable flap without sacrifice of major arteries.It can be used reliably for instep,heel,ankle and distal one third anterior tibial region.

Acoustic Evaluation of Three Vocal Fold Diseases in Pre - and Post - Laryngeal Microsurgery / 听力学及言语疾病杂志

Objective To evaluate acoustic effects of laryngeal microsurgery on vocal polyp, vocal cyst and vocal leuko- plakia. Methods 300 cases in vocal fold diseases were measured acoustically pre - and post - microsurgery. Results After openation, jitter, shimmer and NNE decreased statistically in vocal polyp and vocal cyst patients, and did not improve in vocal leukoplakia patients. Whether or not the surgery was done, F0 of all patients did not change Conclusion Laryngeal microsurgery had definite effect on clearing benign focus. After surgery, vocal functioin improved in vocal polyp and cyst patients, and in vocal leukoplakia, there was no improvement in such a short time as one week.

EGG Measurement of Unilateral Vocal Cord Paralysis / 听力学及言语疾病杂志

To study the effect of unilateral vocal cord paralysis on vocal vibration, EGG parameters of 46 patients were analysed. EGG of most patients were abnormal, CQ decreasing while CI, CQP and CIP increasing. The incidence of normal EGG in patients whose vocal cord located in the middle was higher than that in the para - middle. Inversely, the incidence of abnormal EGG - CQ of the former was lower than that of the latter. EGG of the group in the intermediate was abnormal. The position of paralyzed vocal cord had no effect on CI,CQP and CIP. EGG reflects the characteristics of per vibratory cycle, and is a potential technique to study the irregular vocal vibration.

Effects of Electroglottography on Screening Vocal Fold Disorders / 听力学及言语疾病杂志

Objective To study the effects of Electroglottography(EGG) on screening vocal fold disorders.Methods 134 cases of glottal carcinoma and 167 benign vocal diseases and 62 healthy volunteers were measured. EGG waveform and variables were analyzed to investigate the sensitivity and specificity of EGG measurement.Results Compared with healthy people, the majority of EGG waveform and variables of vocal fold disorders were abnormal. The differences were significant statistically either between healthy people and vocal fold disorders or between benign vocal diseases and malignancy. In benign vocal diseases, the differences of EGG waveform and variables among vocal polyp and cyst and leukoplakia were insignificant statistically, and nor were the differences among various clinical T stages in glottal carcinoma.Conclusion EGG measurement is sensitive to screen vocal fold disorders, and is specific to distinguish benign and malignant diseases, but it couldn not be used to identify the types or clinical stage of the diseases.