1.
Bioorg Med Chem
; 22(20): 5692-6, 2014 Oct 15.
Artigo
em Inglês
| MEDLINE
| ID: mdl-24984939
RESUMO
An in situ H2O2 generation approach to promote P450 peroxygenases catalysis was developed through the use of the nicotinamide cofactor analogue 1-benzyl-1,4-dihydronicotinamide (BNAH) and flavin mononucleotide (FMN). Final productivity could be enhanced due to higher enzyme stability at low H2O2 concentrations. The H2O2 generation represented the rate-limiting step, however it could be easily controlled by varying both FMN and BNAH concentrations. Further characterization can result in an optimized ratio of FMN/BNAH/O2/biocatalyst enabling high reaction rates while minimizing H2O2-related inactivation of the enzyme.
Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Peróxido de Hidrogênio/metabolismo , Bacillus subtilis/enzimologia , Clostridium acetobutylicum/enzimologia , Sistema Enzimático do Citocromo P-450/química , Dinitrocresóis/química , Dinitrocresóis/metabolismo , Peróxido de Hidrogênio/química , Modelos Moleculares , Estrutura Molecular
2.
Angew Chem Int Ed Engl
; 50(45): 10716-9, 2011 Nov 04.
Artigo
em Inglês
| MEDLINE
| ID: mdl-21922621