[Grape seed proanthocyanidin extracts inhibit lipopolysaccharide of Porphyromonas gingivalis].

PURPOSE: To study the inhibitory effect of grape seed proanthocyanidin extracts on 1ipopolysacharides of Porphyromonas gingivalis. METHODS: Grape seed proanthocyanidin extracts were double-diluted to different concentrations by two-fold dilution. The effect on Porphyromonas gingivalis was measured by minimal inhibitory concentration (MIC). The inhibition effect of grape seed proanthocyanidin extracts on lipopolysaccharide was measured by limulus assay with 6 concentrations below the minimal inhibitory concentration. SPSS 17.0 software package was used for statistical analysis. RESULTS: The value of MIC of grape seed proanthocyanidin extracts was 0.8 mg/mL to the strains of the experimental bacteria. The inhibitory effect on lipopolysaccharide enhanced with the increasing of the concentration of grape seed proanthocyanidin extracts within the scope of 0.05 to 0.4 mg/mL (P<0.05,0.01). CONCLUSIONS: Grape seed proanthocyanidin extracts have inhibitory effect on lipopolysaccharide of Porphyromonas gingivalis.

[Application of participatory teaching mode in oral health education].

PURPOSE: To apply participatory teaching mode in oral health education, and to assess its role in cultivating comprehensive stomatological professionals suitable for the development of modern medicine. METHODS: Sixty undergraduate students from grade 2005 in Stomatological College of Nanchang University were selected. Among those students, oral health education course was carried out by traditional teaching mode, while 120 undergraduate students from grades 2006 to 2007 received participatory teaching approach, which paid attention to practice in oral health education practice course. After the course, a survey and evaluation of teaching effectiveness was conducted. RESULTS: Questionnaire survey showed that participatory teaching mode could significantly improve the students' capabilities and provide much more help to their study. CONCLUSIONS: Application of participatory teaching mode in oral health education course for undergraduates is feasible. It can improve students' comprehensive ability and cultivate their cultural literacy and scientific literacy. It also meets the training goal of stomatological professionals and the development trend of education reform. Supported by Higher School Teaching Reform Research Subject of Jiangxi Province(JXJG-10-1-42).