RESUMO
In this study, the presence of genes responsible for the pathogenicity and antibiotic resistance profile of enterococci isolated from various foodstuffs of animal origin was investigated. The percentage prevalence of enterococci was 54.1% (203/375) and the average count was found to be 3.81 log cfu/ml-g. Species-specific primers revealed Enterococcus faecalis as the predominant species carrying one or more virulence-associated traits of efa, gelE, ace, esp and agg genetic markers. Only one E. faecium isolate (from milk) was positive for the esp gene. Regarding antibiotic resistance, the highest frequency of resistance was observed for tetracycline (21.7%), followed by quinupristin/dalfopristin (13.3%), ciprofloxacin (2.0%), penicillin (2.0%), linezolid (1.0%), ampicillin (1.0%), streptomycin (1.0%), and gentamicin (0.5%). Enterococcus faecalis showed a higher prevalence of antibiotic resistance than other enterococci. The percentage of multidrug resistance among the isolates was 3.4%. Twenty-nine E. faecalis isolates (26.6%) carrying one of the virulence-associated traits were at the same time resistant to at least one antibiotic. Our results show that foods of animal origin, including ready-to-eat products, may be reservoirs of antibiotic-resistant and potentially virulent enterococci.
Assuntos
Antibacterianos/farmacologia , Laticínios/microbiologia , Farmacorresistência Bacteriana , Enterococcus/efeitos dos fármacos , Carne/microbiologia , Animais , Bovinos , Microbiologia de Alimentos , Testes de Sensibilidade Microbiana , Aves Domésticas , TurquiaRESUMO
Growth performance, carcass characteristics, post-slaughtering and haematological parameters of Kivircik and Karacabey Merino male lambs in conventional and organic management systems were compared. The animals which were weaned at 7 weeks of age were divided into Kivircik conventional, Kivircik organic (KO), Karacabey Merino conventional and Karacabey Merino organic (MO) groups containing 12 lambs each. Fattening was ended when lambs attained 35 kg of live weight. The time to attain the determined fattening weight was significantly different among the groups, and Merino lambs having higher live weight gain were earlier than Kivircik lambs (p < 0.05). Overall conventional (CG) and organic group lambs were also compared. Live weight gain, intra-abdominal fat amount, external fat thickness and visceral organ weight were significantly higher in CG lambs (p < 0.05). Higher haematocrit and erythrocyte counts were obtained with the CG group (p < 0.05), whilst triglyceride, total plasma cholesterol and lipoprotein (HDL, LDL, VLDL) levels between groups were not significant. Pneumonia was the unique infection, with an incidence of 50% (six lambs) and 16.6% (two lambs) for MO and KO animals, respectively. The mortality rate was 16.6% (two lambs) for MO group, whilst no mortality was recorded for KO group animals. The present study has shown that although Karacabey merino lambs had higher growth performance compared to Kivircik lambs, organically fattened lambs in whole exhibited inferior growth performance. Lower infection and mortality observed with Kivircik lambs suggested that they could be more resistant to infections and outdoor environmental conditions.
Assuntos
Criação de Animais Domésticos/métodos , Linhagem , Carneiro Doméstico/crescimento & desenvolvimento , Aumento de Peso/genética , Tecido Adiposo/crescimento & desenvolvimento , Análise de Variância , Fenômenos Fisiológicos da Nutrição Animal , Animais , Genótipo , Hematologia , Masculino , Agricultura Orgânica , Carneiro Doméstico/classificação , Carneiro Doméstico/genética , Especificidade da Espécie , TurquiaRESUMO
Non-feed removal molting programme in commercial brown laying hens and its influence on pre-molting, post-molting and end of cycle egg quality traits were investigated. Overall 54 birds were randomly divided into three treatment groups and each group was fed with one of the following diets during 10 days of molting period: (i) grain barley, (ii) alfalfa meal, or (iii) commercial layer ration (non-molted control group). Eggs obtained from groups in pre-molting, post-molting and end of cycle periods were examined for several quality performance traits such as egg weight, specific gravity, shape index, shell strength, shell thickness, eggshell weight, haugh unit, albumen index, yolk index and yolk color. Results indicated that non-feed removal molting programme based particularly on grain barley had positive effect on egg quality traits in laying hens. Notably, yolk color and haugh unit, which are considered as the most important quality parameters from the consumer point of view, were relatively improved in barley molted group.
Assuntos
Ração Animal , Galinhas/fisiologia , Ovos/normas , Hordeum , Medicago sativa , Muda/fisiologia , Criação de Animais Domésticos/métodos , Animais , Feminino , Oviposição/fisiologia , Distribuição Aleatória , TurquiaRESUMO
Honey samples, collected from the Southern Marmara region of Turkey, were analysed for erythromycin residues by liquid chromatography-mass spectrometry using electrospray ionization in the positive ion mode (LC-ESI-MS). Fifty samples, comprising chestnut, pine, linden and multi-flower honeys, were collected directly from hives and analyzed. The limit of detection and quantification were 6 and 20 ng g(-1), respectively, and recovery ranged from 85 to 89%. Four of the honey samples (8%) were found to be contaminated with erythromycin residues at concentrations ranging from 50 to 1776 ng g(-1). An erythromycin-fortified cake feeding assay was also performed in a defined hive to test the transfer of erythromycin residue to the honey matrix. In this test hive, the residue level in the honey, 3 months after dosing, was approximately 28 ng g(-1).