Culture of human cell lines by a pathogen-inactivated human platelet lysate.

Alternatives to the use of fetal bovine serum (FBS) have been investigated to ensure xeno-free growth condition. In this study we evaluated the efficacy of human platelet lysate (PL) as a substitute of FBS for the in vitro culture of some human cell lines. PL was obtained by pools of pathogen inactivated human donor platelet (PLT) concentrates. Human leukemia cell lines (KG-1, K562, JURKAT, HL-60) and epithelial tumor cell lines (HeLa and MCF-7) were cultured with either FBS or PL. Changes in cell proliferation, viability, morphology, surface markers and cell cycle were evaluated for each cell line. Functional characteristics were analysed by drug sensitivity test and cytotoxicity assay. Our results demonstrated that PL can support growth and expansion of all cell lines, although the cells cultured in presence of PL experienced a less massive proliferation compared to those grown with FBS. We found a comparable percentage of viable specific marker-expressing cells in both conditions, confirming lineage fidelity in all cultures. Functionality assays showed that cells in both FBS- and PL-supported cultures maintained their normal responsiveness to adriamycin and NK cell-mediated lysis. Our findings indicate that PL is a feasible serum substitute for supporting growth and propagation of haematopoietic and epithelial cell lines with many advantages from a perspective of process standardization, ethicality and product safety.

Changes induced by KI administration on plasma heparinic activity in healthy subjects.

Other researches WE HAVE carried out show how it is possible to correct the high level of neutral fats in subjects with high trygliceridemia, with the administration of KI. We wished to ascertain whether the capacity of KI to reduce trygliceridemia when this is increased should be correlated to a possible change induced by this salt on PHA. Our researches has been carried out on 11 subjects who received a daily dose of 28 mg/Kg of b.w. of KI for a period of 10 days. The result we have obtained indicates that the administration of KI does not cause any significant change of PHA both after load and after prolonged treatment with KI.