RESUMO
The most deadly outbreak of Escherichia coli O104:H4 occurred in Europe in 2011. Here, we evaluated the effects of the retrograde trafficking inhibitor Retro-2(cycl) in a murine model of E. coli O104:H4 infection. Systemic treatment with Retro-2(cycl) significantly reduced body weight loss and improved clinical scores and survival rates for O104:H4-infected mice. The present data established that Retro-2(cycl) contributes to the protection of mice against O104:H4 infection and may represent a novel approach to limit Shiga toxin-producing Escherichia coli (STEC)-induced toxicity.
Assuntos
Benzamidas/farmacologia , Escherichia coli Êntero-Hemorrágica/efeitos dos fármacos , Infecções por Escherichia coli/tratamento farmacológico , Síndrome Hemolítico-Urêmica/tratamento farmacológico , Toxina Shiga II/antagonistas & inibidores , Tiofenos/farmacologia , Animais , Benzamidas/uso terapêutico , Chlorocebus aethiops , Modelos Animais de Doenças , Surtos de Doenças , Escherichia coli Êntero-Hemorrágica/genética , Escherichia coli Êntero-Hemorrágica/patogenicidade , Infecções por Escherichia coli/epidemiologia , Europa (Continente) , Células HeLa , Síndrome Hemolítico-Urêmica/prevenção & controle , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Tiofenos/uso terapêutico , Células VeroRESUMO
A general strategy to localize and quantify carbon-centered radicals within proteins is described. The methodology was first exemplified on amino acids and then on a peptide. This method is applicable to any protein system regardless of size, and the site of hydrogen abstraction by *OH on all residues within proteins is easily and accurately detected.
Assuntos
Aminoácidos/química , Carbono/análise , Proteínas/química , Animais , Radicais Livres/análise , Peptídeos/química , Trítio/análiseRESUMO
The Stille cross-coupling reaction of an N-tosyl alpha-stannyl enamine is clearly exemplified for the first time with a wide range of halogeno derivatives. This gives access to functionalized alpha-substituted enamines in fair yields.