RESUMO
Autophagosomes primarily mediate turnover of cytoplasmic proteins or organelles to provide nutrients and eliminate damaged proteins. In neurons, autophagosomes form in distal axons and are trafficked retrogradely to fuse with lysosomes in the soma. Although defective neuronal autophagy is associated with neurodegeneration, the function of neuronal autophagosomes remains incompletely understood. We show that in neurons, autophagosomes promote neuronal complexity and prevent neurodegeneration in vivo via retrograde transport of brain-derived neurotrophic factor (BDNF)-activated TrkB receptors. p150Glued/dynactin-dependent transport of TrkB-containing autophagosomes requires their association with the endocytic adaptor AP-2, an essential protein complex previously thought to function exclusively in clathrin-mediated endocytosis. These data highlight a novel non-canonical function of AP-2 in retrograde transport of BDNF/TrkB-containing autophagosomes in neurons and reveal a causative link between autophagy and BDNF/TrkB signalling.
Assuntos
Complexo 2 de Proteínas Adaptadoras/metabolismo , Encéfalo/patologia , Receptor trkB/metabolismo , Animais , Autofagossomos , Autofagia , Transporte Biológico , Encéfalo/metabolismo , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Complexo Dinactina/metabolismo , Endocitose , Camundongos , Camundongos Knockout , Proteínas Associadas aos Microtúbulos/metabolismo , Neurônios/metabolismo , Ligação Proteica , Ratos Wistar , Transdução de SinaisRESUMO
Neuronal replacement in the pallial song control nucleus HVC of adult zebra finches constitutes an interesting case of homeostatic plasticity; in spite of continuous addition and attrition of neurons in ensembles that code song elements, adult song remains remarkably invariant. New neurons migrate into HVC and later synapse with their target, arcopallial song nucleus RA (HVCRA). New HVCRA neurons respond to auditory stimuli (in anaesthetised animals), but whether and when they become functionally active during singing is unknown. We studied this, using 5-bromo-2'-deoxyuridine to birth-date neurons, combined with immunohistochemical detection of immediate-early gene (IEG) expression and retrograde tracer injections into RA to track connectivity. Interestingly, singing was followed by IEG expression in a substantial fraction of new neurons that were not retrogradely labelled from RA, suggesting a possible role in HVC-intrinsic network function. As new HVC neurons matured, the proportion of HVCRA neurons that expressed IEGs after singing increased significantly. Since it was previously shown that singing induces IEG expression in HVC also in deaf birds and that hearing song does not induce IEG expression in HVC, our data provide the first direct evidence that new HVC neurons are engaged in song motor behaviour.
Assuntos
Percepção Auditiva/fisiologia , Centro Vocal Superior/fisiologia , Neurogênese , Plasticidade Neuronal , Neurônios/fisiologia , Vocalização Animal , Estimulação Acústica , Animais , Proteínas Aviárias/metabolismo , Proteína 1 de Resposta de Crescimento Precoce/metabolismo , Tentilhões , Masculino , Vias Neurais/metabolismo , Vias Neurais/fisiologia , Neurônios/metabolismo , Proteínas Proto-Oncogênicas c-fos/metabolismoRESUMO
Neurotransmission depends on presynaptic membrane retrieval and local reformation of synaptic vesicles (SVs) at nerve terminals. The mechanisms involved in these processes are highly controversial with evidence being presented for SV membranes being retrieved exclusively via clathrin-mediated endocytosis (CME) from the plasma membrane or via ultrafast endocytosis independent of clathrin. Here we show that clathrin and its major adaptor protein 2 (AP-2) in addition to the plasma membrane operate at internal endosome-like vacuoles to regenerate SVs but are not essential for membrane retrieval. Depletion of clathrin or conditional knockout of AP-2 result in defects in SV reformation and an accumulation of endosome-like vacuoles generated by clathrin-independent endocytosis (CIE) via dynamin 1/3 and endophilin. These results together with theoretical modeling provide a conceptual framework for how synapses capitalize on clathrin-independent membrane retrieval and clathrin/AP-2-mediated SV reformation from endosome-like vacuoles to maintain excitability over a broad range of stimulation frequencies.
Assuntos
Complexo 2 de Proteínas Adaptadoras/fisiologia , Clatrina/fisiologia , Invaginações Revestidas da Membrana Celular/fisiologia , Endocitose , Hipocampo/fisiologia , Sinapses/fisiologia , Vesículas Sinápticas/fisiologia , Complexo 2 de Proteínas Adaptadoras/genética , Animais , Clatrina/genética , Invaginações Revestidas da Membrana Celular/ultraestrutura , Dinaminas/metabolismo , Endossomos/fisiologia , Endossomos/ultraestrutura , Hipocampo/ultraestrutura , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Modelos Teóricos , Neurônios/fisiologia , Neurônios/ultraestrutura , Ratos , Sinapses/ultraestrutura , Vesículas Sinápticas/ultraestruturaRESUMO
Synaptic vesicle recycling has long served as a model for the general mechanisms of cellular trafficking. We used an integrative approach, combining quantitative immunoblotting and mass spectrometry to determine protein numbers; electron microscopy to measure organelle numbers, sizes, and positions; and super-resolution fluorescence microscopy to localize the proteins. Using these data, we generated a three-dimensional model of an "average" synapse, displaying 300,000 proteins in atomic detail. The copy numbers of proteins involved in the same step of synaptic vesicle recycling correlated closely. In contrast, copy numbers varied over more than three orders of magnitude between steps, from about 150 copies for the endosomal fusion proteins to more than 20,000 for the exocytotic ones.