1.
Appl Environ Microbiol
; 75(4): 1211-4, 2009 Feb.
Artigo
em Inglês
| MEDLINE
| ID: mdl-19098221
RESUMO
We developed a novel negative selection system for actinobacteria based on cytosine deaminase (CodA). We constructed vectors that include a synthetic gene encoding the CodA protein from Escherichia coli optimized for expression in Streptomyces species. Gene disruption and the introduction of an unmarked in-frame deletion were successfully achieved with these vectors.