Replication in cultured C2C12 muscle cells correlates with the neuroinvasiveness of California serogroup bunyaviruses.

The neuroinvasiveness of California serogroup bunyaviruses is determined by the ability of the virus to replicate in striated muscle after peripheral inoculation of mice. Neuroinvasiveness was mapped to the medium (M) RNA segment of the virus, which encodes the viral glycoproteins, when reassortants were made between La Crosse/original virus, a neuroinvasive isolate, and Tahyna-181/57 virus, a nonneuroinvasive clone. We have tested the murine muscle cell line C2C12 as a surrogate for myotropism and have found that there is a slight, but reproducible difference in the replication of virus clones bearing the M RNA segment of La Crosse/original virus compared to clones bearing the M RNA segment of Tahyna-181/57 virus, as determined by viral titer, antigen expression, and plaque formation.

Structure of L-prolyl-L-tyrosine monohydrate.

C14H18N2O4.H2O, Mr = 296.32, triclinic, P1, a = 5.524 (3), b = 6.621 (2), c = 10.307 (2) A, alpha = 78.82 (3), beta = 86.82 (4), gamma = 84.96 (4) degrees, V = 368.11 A3, Z = 1, Dx = 1.34 g cm-3, lambda(Mo K alpha 1) = 0.70930 A, mu = 1.10 cm-1, F(000) = 158, T = 298 K, final R = 0.044 for 2182 observed reflections. The molecule crystallizes as a zwitterion with the peptide backbone folded and a water molecule of hydration. The water molecule and the dipeptide molecule are involved in an extensive hydrogen-bond network.