Microplastic Textile Fibers Accumulate in Sand and Are Potential Sources of Micro(nano)plastic Pollution.

Agricultural soils have been identified as sinks for microplastic fibers; however, little information is available on their long-term fate in these soils. In this study, polyester and nylon fibers were precisely cut to relevant environmental lengths, using novel methodology, and their behavior in sand columns was studied at environmental concentration. The longer fibers (>50 µm) accumulated in the upper layers of the sand, smaller fibers were slightly more mobile, and nylon showed marginally higher mobility than polyester. Previous studies have overlooked changes in microplastic morphology due to transport in soil. Our study is the first to show that fibers exhibited breakage, peeling, and thinning under flow conditions in soil, releasing smaller, more mobile fragments. Furthermore, the peelings exhibited different adsorption properties compared to the core fiber. This suggests that microplastic fibers can become a source of smaller micro(nano)plastics and potential vectors for certain molecules, risking continuous contamination of nearby soils, surfaces, and groundwater.

Curli production enhances clay-E. coli aggregation and sedimentation.

Curli are amyloid fibrils that polymerize extracellularly from curlin, a protein that is secreted by many enteric bacteria and is important for biofilm formation. Presented here is a systematic study of the effects of curli on bacteria-clay interactions. The aggregation trends of curli-producing and curli-deficient bacteria with clay minerals were followed using gradient-sedimentation experiments, Lumisizer measurements, bright-field and electron microscopy. The results revealed that curli-producing bacteria auto-aggregated into high-density flocs (1.23 g/cm3), ranging in size from 10 to 50 µm, that settle spontaneously. In contrast, curli-deficient bacteria remained relatively stable in solution as individual cells (1-2 µm, 1.18 g/cm3), even at high ionic strength (350 mM). The stability of clay suspensions mixed with curli-deficient bacteria depended on clay type and ionic strength, the general trends being consistent with the classic DLVO theory. However, suspensions of curli-producing bacteria mixed with clays were highly unstable regardless of clay type and solution chemistry, suggesting extensive interactions between the clays and the bacteria-curli aggregates. SEM measurements revealed interesting differences in morphologies of the aggregates; montmorillonite particles coated the bacterial auto-aggregates whereas the kaolinite platelets were embedded within the larger curli-bacteria aggregates. These new observations regarding the densities, aggregation trends, and morphologies of bacteria-curli and bacteria-curli-clay complexes make it clear that production of surface appendages, such as curli, need to be considered when addressing the fate, activity and transport of bacteria - particularly in aquatic environments.