RESUMO
This study reports significant steps toward developing anti-biofilm surfaces based on superhydrophobic properties that meet the complex demands of today's food and medical regulations. It presents inverse Pickering emulsions of water in dimethyl carbonate (DMC) stabilized by hydrophobic silica (R202) as a possible food-grade coating formulation and describes its significant passive anti-biofilm properties. The final coatings are formed by applying the emulsions on the target surface, followed by evaporation to form a rough layer. Analysis shows that the final coatings exhibited a Contact Angle (CA) of up to 155° and a Roll-off Angle (RA) lower than 1° on the polypropylene (PP) surface, along with a relatively high light transition. Dissolving polycaprolactone (PCL) into the continuous phase enhanced the average CA and coating uniformity but hindered the anti-biofilm activity and light transmission. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed a uniform coating by a "Swiss-cheese" like structure with high nanoscale and microscale roughness. Biofilm experiments confirm the coating's anti-biofilm abilities that led to the reduction in survival rates of S.aureus and E.coli, by 90-95% respectively, compared to uncoated PP surfaces.
Assuntos
Biofilmes , Staphylococcus aureus , Emulsões/química , Interações Hidrofóbicas e Hidrofílicas , ÁguaRESUMO
This study presents a new eco-friendly formulation of entomopathogenic nematodes (EPNs) based on individual coating of EPNs with titanium dioxide (TiO2) nanoparticles (NPs) and mineral oil via oil-in-water Pickering emulsions. Mineral oil-in-water emulsions stabilized by amine-functionalized titanium dioxide (TiO2-NH2) particles were prepared. 40:60 and 50:50 oil-water volume ratios using 2 wt % TiO2-NH2 particles were found to be the most stable emulsions with a droplet size suitable for the formulation and were further studied for their toxicity against the incorporated EPNs. Carboxyfluorescein was covalently bonded to TiO2-NH2 NPs, and the resulting composite was observed via fluorescence confocal microscopy. The dry coating was evaluated using SEM and confocal microscopy, which showed significant nematode coverage by the particles and oil. The final formulation was biocompatible with the studied EPNs, where the viability of the EPNs in the formulation was equivalent to control aqueous suspension after 120 days. Finally, yields of nematodes from infected Galleria mellonella cadavers collected for 150 days showed no significant differences (P > 0.05) using the tested emulsions compared to the control containing nematodes in water.
Assuntos
Nanopartículas , Nematoides , Animais , Emulsões , Agentes de Controle Biológico , Óleo Mineral , Água , Aminas , Tamanho da PartículaRESUMO
In a series of six experiments, the method of magnitude estimation, constrained by a multivariate model, was used to assess the rules that govern the summation of the loudness of two-tone complexes. This methodology enabled us to specify the amounts of summation and simultaneously to construct the corresponding loudness scales. The components had different frequency separations and in the different experiments were presented (1) dichotically, a different frequency to each ear; (2) diotically, to both ears; and (3) monaurally. Results replicated and in some conditions extended known features of multiple signal processing by the auditory system. Thus, qualitatively different rules of loudness integration appeared. For monaural and diotic modes of stimulation, overall loudness depended on total sound energy within the critical band, but on the simple sum of component loudnesses beyond the critical band. For dichotic presentations, a fully additive rule of loudness summation appeared, regardless of frequency spacing. For the latter (but not the former), loudness summation was perfect, with the underlying loudness scales closely approximating Stevens's sone scale.
Assuntos
Dominância Cerebral , Hiperacusia , Discriminação da Altura Tonal , Atenção , Limiar Auditivo , Testes com Listas de Dissílabos , Humanos , PsicoacústicaRESUMO
Subjects judged the loudness of tones (Experiment 1) and of bursts of noise (Experiment 2) that varied in intensity and duration as well as in mode of presentation (monaural vs. binaural). Both monaural and binaural loudness, for both types of signals, obeyed the bilinear-interaction prediction of the classic temporal integration model. The loudness of short tones grows as a power function of both intensity and duration with different exponents for the two factors (.2 and .3, respectively). The loudness of wide-band noises grows as a power function of duration (with an exponent of approximately .6) but not of sound pressure. For tones, binaural summation was constant but fell short of full additivity. For noises, summation changed across level and duration. Temporal summation followed the same course for monaural and binaural tonal stimuli but not for noise stimuli. Notwithstanding these differences between tone and noise, we concluded that binaural and temporal summation are independently operating integrative networks within the auditory system. The usefulness of establishing the underlying metric structure for temporal summation is emphasized.
Assuntos
Dominância Cerebral , Percepção Sonora , Ruído , Discriminação da Altura Tonal , Percepção do Tempo , Adulto , Atenção , Feminino , Humanos , Masculino , PsicoacústicaRESUMO
Five subjects were required to judge the loudness of pure tones that varied in intensity and duration (Experiment 1), in intensity and frequency (Experiment 2), or in duration and frequency (Experiment 3). Results of all three experiments were consistent with the respective models of sensory integration expected to underlie the various judgmental tasks (multiplicative for the first experiment, unidimensional for the latter two). Despite the use of common stimulus values across experiments and instructions to judge solely loudness, different loudness scales emerged. This outcome supports a task dependent, multirepresentative scheme for loudness.
Assuntos
Percepção Sonora , Adulto , Análise de Variância , Feminino , Humanos , Julgamento , Masculino , Modelos Neurológicos , Modelos PsicológicosRESUMO
Subjects judged the loudness and the lateral position of dichotic transient signals, which were presented at equal and unequal levels, synchronously and asynchronously, to the two ears. Binaural loudness summation of clicks does not obey a law of linear addition: It is partial at low level and superadditive at high level. Supersummation is greater for interaurally delayed clicks than for coincidental ones. The relation between click loudness and sound pressure (over moderate SLs) can be described as a power function with a greater exponent for the binaural function. Lateral positions spread over a greater range for interaural level differences than for interaural time differences. The time-intensity trading ratio was greater than is typically reported for tones. When sound lateralization was induced by interaural time difference, but not by intensity difference, a virtually perfect negative correlation between loudness and extent of off-center displacement existed.
Assuntos
Atenção , Dominância Cerebral , Percepção Sonora , Limiar Auditivo , Humanos , PsicoacústicaRESUMO
Subjects made magnitude estimations of noxious stimuli produced by a 6 X 6 factorial design of electric shocks (pulse trains) and loud tones. Group data and all individual results conformed to a linear additive model of pain. The estimates of pain approximated the linear sum of the pain estimates of the individual electrocutaneous and auditory components. Pain related differently to the two inducing stimuli. It grew as a mildly expansive power function of current intensity (with an exponent of about 1.2) but as a mildly compressive power function of sound-pressure level (with an exponent of about 0.8). These results replicate recent findings by the same authors in 1986 using a more aversive type of electric stimulation. They are interpreted as supportive of a new functional approach to understand pain and pain-related phenomena.
Assuntos
Dor/psicologia , Sensação , Humanos , Masculino , Medição da Dor , Psicofísica , Limiar SensorialRESUMO
A symmetrical 6 x 6 factorial design of distances and durations served to produce either 36 different moving stimuli (real movement condition) or 36 static displays separately containing the respective stimulus components (cognitive movement condition). Different metric rules underlay the two types of velocity judgments: Perceptual estimations of real movement obeyed a ratio model, whereas conscious estimations of implied movement obeyed an additive model. Valuation operations differed, too; the scales underlying real velocity were nonlinearly related to the even more compressive scales that underlay cognitive velocity. Implications of these results for velocity research are discussed.
Assuntos
Cognição , Percepção de Movimento , Adulto , Aprendizagem por Discriminação , Percepção de Distância , Humanos , PsicofísicaRESUMO
Functional measurement analyses and psychophysical techniques were used to assess how separate, cross-modal, aversive events are integrated in judgements of pain. Subjects made magnitude estimations of noxious stimuli produced by a 6 X 6 factorial design of electric shocks and loud tones. Group data and most of the individual results were consistent with a model of linear pain summation: The estimates of pain approximated the linear sum of the pain estimates of the individual electrocutaneous and auditory components. The relation between painful sensation and current intensity could be described by a mildly expansive power function with an exponent of about 1.1. Auditorily produced painful sensation related to sound pressure level by a mildly compressive power function with an exponent of about 0.90 as a representative figure. Results are interpreted in terms of a functional theory of pain. Noxious events are first transformed to psychological scale values via stimulus-specific psychophysical transfer functions. The outputs of these functions are then combined with other pain-related internal representations of either sensory or cognitive origin, according to simple algebraic models.
Assuntos
Dor/psicologia , Estimulação Acústica , Adulto , Sistema Nervoso Central/fisiopatologia , Eletrochoque , Feminino , Humanos , Masculino , Modelos Psicológicos , Dor/fisiopatologia , PsicofísicaRESUMO
Subjects made magnitude estimations of moving stimuli produced by a 10 X 10 factorial design of distances and durations. Both group and individual data obeyed the bilinear interaction prediction of a simple ratio model. The relation between perceived and actual velocity, as well as the psychophysical contingencies constructed from the marginal means of the design, could be described by a power function with an exponent of about 0.63 as a representative figure. Plotting subjective velocity against physical velocity with either duration or distance as the parameter resulted, respectively, in families of converging psychophysical power functions. Some implications of the results for velocity research, especially the usefulness of specifying the correct metric structure, are discussed.