Prior experience with behavioral control over stress facilitates social dominance.

Dominance status has extensive effects on physical and mental health, and an individual's relative position can be shaped by experiential factors. A variety of considerations suggest that the experience of behavioral control over stressors should produce winning in dominance tests and that winning should blunt the impact of later stressors, as does prior control. To investigate the interplay between competitive success and stressor control, we first examined the impact of stressor controllability on subsequent performance in a warm spot competition test modified for rats. Prior experience of controllable, but not physically identical uncontrollable, stress increased later effortful behavior and occupation of the warm spot. Controllable stress subjects consistently ranked higher than did uncontrollable stress subjects. Pharmacological inactivation of the prelimbic (PL) cortex during behavioral control prevented later facilitation of dominance. Next, we explored whether repeated winning experiences produced later resistance against the typical sequelae of uncontrollable stress. To establish dominance status, triads of rats were given five sessions of warm spot competition. The development of stable dominance was prevented by reversible inactivation of the PL or NMDA receptor blockade in the dorsomedial striatum. Stable winning blunted the later stress-induced increase in dorsal raphe nucleus serotonergic activity, as well as prevented uncontrollable stress-induced social avoidance. In contrast, endocrine and neuroimmune responses to uncontrollable stress were unaffected, indicating a selective impact of prior dominance. Together, these data demonstrate that instrumental control over stress promotes later dominance, but also reveal that winning experiences buffer against the neural and behavioral outcomes of future adversity.

Prior experience with behavioral control over stress facilitates social dominance.

Dominance status has extensive effects on physical and mental health, and an individual's relative position can be shaped by experiential factors. A variety of considerations suggest that the experience of behavioral control over stressors should produce winning in dominance tests and that winning should blunt the impact of later stressors, as does prior control. To investigate the interplay between competitive success and stressor control, we first examined the impact of stressor controllability on subsequent performance in a warm spot competition test modified for rats. Prior experience of controllable, but not physically identical uncontrollable, stress increased later effortful behavior and occupation of the warm spot. Controllable stress subjects consistently ranked higher than did uncontrollable stress subjects. Pharmacological inactivation of the prelimbic (PL) cortex during behavioral control prevented later facilitation of dominance. Next, we explored whether repeated winning experiences produced later resistance against the typical sequelae of uncontrollable stress. To establish dominance status, triads of rats were given five sessions of warm spot competition. Reversible inactivation of the PL or NMDA receptor blockade in the dorsomedial striatum led to a long-term reduction in social rank. Stable dominance blunted the later stress-induced increase in dorsal raphe nucleus serotonergic activity, as well as prevented stress-induced social avoidance. In contrast, endocrine and neuroimmune responses to uncontrollable stress were unaffected, indicating a selective impact of prior dominance. Together, these data demonstrate that instrumental control over stress promotes later dominance, but also reveal that winning experiences buffer against the neural and behavioral outcomes of future adversity.

Novel RAF Fusions in Pediatric Low-Grade Gliomas Demonstrate MAPK Pathway Activation.

Brain tumors are the most common solid tumor in children, and low-grade gliomas (LGGs) are the most common childhood brain tumor. Here, we report on 3 patients with LGG harboring previously unreported or rarely reported RAF fusions: FYCO1-RAF1, CTTNBP2-BRAF, and SLC44A1-BRAF. We hypothesized that these tumors would show molecular similarity to the canonical KIAA1549-BRAF fusion that is the most widely seen alteration in pilocytic astrocytoma (PA), the most common pediatric LGG variant, and that this similarity would include mitogen-activated protein kinase (MAPK) pathway activation. To test our hypothesis, we utilized immunofluorescent imaging and RNA-sequencing in normal brain, KIAA1549-BRAF-harboring tumors, and our 3 tumors with novel fusions. We performed immunofluorescent staining of ERK and phosphorylated ERK (p-ERK), identifying increased p-ERK expression in KIAA1549-BRAF fused PA and the novel fusion samples, indicative of MAPK pathway activation. Geneset enrichment analysis further confirmed upregulated downstream MAPK activation. These results suggest that MAPK activation is the oncogenic mechanism in noncanonical RAF fusion-driven LGG. Similarity in the oncogenic mechanism suggests that LGGs with noncanonical RAF fusions are likely to respond to MEK inhibitors.

An experimental study on transfer function estimation using acoustic modelling and singular value decomposition.

Transfer functions relating sound source strengths and the sound pressure at field points are important for sound field control. Recently, two modal domain methods for transfer function estimation have been compared using numerical simulations. One is the spatial harmonic decomposition (SHD) method, which models a sound field with a series of cylindrical waves; while the other is the singular value decomposition (SVD) method, which uses prior sound source location information to build an acoustic model and obtain basis functions for sound field modelling. In this paper, the feasibility of the SVD method using limited measurements to estimate transfer functions over densely spaced field samples within a target region is demonstrated experimentally. Experimental results with various microphone placements and system configurations are reported to demonstrate the geometric flexibility of the SVD method compared to the SHD method. It is shown that the SVD method can estimate broadband transfer functions up to 3099 Hz for a target region with a radius of 0.083 m using three microphones, and allow flexibility in system geometry. Furthermore, an application example of acoustic contrast control is presented, showing that the proposed method is a promising approach to facilitating broadband sound zone control with limited microphones.

A comparison between two modal domain methods for personal audio reproduction.

Personal audio provides private and personalized listening experiences by generating sound zones in a shared space with minimal interference between zones. One challenge of the design is to achieve the best performance with a limited number of microphones and loudspeakers. In this paper, two modal domain methods for personal audio reproduction are compared. One is the spatial harmonic decomposition (SHD) based method and the other is the singular value decomposition (SVD) based method. It is demonstrated that the SVD based method provides a more efficient modal domain decomposition than the SHD method for 2.5 dimensional personal audio design. Simulation results show that the SVD based method outperforms the SHD one by up to 10 dB in terms of acoustic contrast and up to 17 dB in terms of reproduction error for a compact arc array with five loudspeakers, while requiring fewer microphones around the zone boundaries. The SVD based method retains the inherent efficiency of optimizing in a modal domain while avoiding the inherent geometric limitations of using SHD basis functions. Thus, this approach is advantageous for applications with flexible system geometries and a small number of loudspeakers and microphones.

Robust reproduction of sound zones with local sound orientation.

Pressure matching (PM) and planarity control (PC) methods can be used to reproduce local sound with a certain orientation at the listening zone, while suppressing the sound energy at the quiet zone. In this letter, regularized PM and PC, incorporating coarse error estimation, are introduced to increase the robustness in non-ideal reproduction scenarios. Facilitated by this, the interaction between regularization, robustness, (tuned) personal audio optimization, and local directional performance is explored. Simulations show that under certain conditions, PC and weighted PM achieve comparable performance, while PC is more robust to a poorly selected regularization parameter.

Optimal source placement for sound zone reproduction with first order reflections.

The problem of delivering personal audio content to listeners sharing the same acoustic space has recently attracted attention. It has been shown that a perceptually acceptable level of acoustic separation between the listening zones is difficult to achieve with active control in non-anechoic conditions. A common problem of strong first order reflections has not been examined in detail for systems with practical constraints. Acoustic contrast maximization combined with optimization of source positions is identified as a potentially effective control strategy when strong individual reflections occur. An analytic study is carried out to describe the relationship between the performance of a 2 × 2 (two sources and two control sensors) system and its geometry in a single-reflection scenario. The expression for acoustic contrast is used to formulate guidelines for optimizing source positions, based on three distinct techniques: Null-Split, Far-Align, and Near-Align. The applicability of the techniques to larger systems with up to two reflections is demonstrated using numerical optimization. Simulation results show that optimized systems produce higher acoustic contrast than non-optimized source arrangements and an alternative method for reducing the impact of reflections (sound power minimization).

Personal audio with a planar bright zone.

Reproduction of multiple sound zones, in which personal audio programs may be consumed without the need for headphones, is an active topic in acoustical signal processing. Many approaches to sound zone reproduction do not consider control of the bright zone phase, which may lead to self-cancellation problems if the loudspeakers surround the zones. Conversely, control of the phase in a least-squares sense comes at a cost of decreased level difference between the zones and frequency range of cancellation. Single-zone approaches have considered plane wave reproduction by focusing the sound energy in to a point in the wavenumber domain. In this article, a planar bright zone is reproduced via planarity control, which constrains the bright zone energy to impinge from a narrow range of angles via projection in to a spatial domain. Simulation results using a circular array surrounding two zones show the method to produce superior contrast to the least-squares approach, and superior planarity to the contrast maximization approach. Practical performance measurements obtained in an acoustically treated room verify the conclusions drawn under free-field conditions.

Acoustic contrast, planarity and robustness of sound zone methods using a circular loudspeaker array.

Since the mid 1990s, acoustics research has been undertaken relating to the sound zone problem-using loudspeakers to deliver a region of high sound pressure while simultaneously creating an area where the sound is suppressed-in order to facilitate independent listening within the same acoustic enclosure. The published solutions to the sound zone problem are derived from areas such as wave field synthesis and beamforming. However, the properties of such methods differ and performance tends to be compared against similar approaches. In this study, the suitability of energy focusing, energy cancelation, and synthesis approaches for sound zone reproduction is investigated. Anechoic simulations based on two zones surrounded by a circular array show each of the methods to have a characteristic performance, quantified in terms of acoustic contrast, array control effort and target sound field planarity. Regularization is shown to have a significant effect on the array effort and achieved acoustic contrast, particularly when mismatched conditions are considered between calculation of the source weights and their application to the system.

A simple technique to estimate the fully time-resolved x-ray diameter of a z pinch.

Occultations are routinely used to derive information about astronomical objects. Here an occultation scheme is used to derive a fully time-resolved estimate of the x-ray emitting diameter of a z pinch. By using different filtrations on the sensors, one could for example, distinguish the size of the K-line emitting region compared to the higher energy K-continuum emitting volume. Or with suitable apertures and detector arrays, the pinch diameter could be axially and temporally resolved.