Impact of the implementation of Identification-Situation-Background-Assessment-Recommendation (ISBAR) tool to improve quality and safety measure in a lithotripsy and endourological unit.

INTRODUCTION: A lack of professional communication and collaboration may be one of the main causes of medication errors. The objective was to evaluate the results of the implementation of ISBAR as a communication and safety tool in a Lithotripsy and Endourologic Unit of a tertiary public hospital. METHODS: A total of 457 patients were included in a retrospective study from 2014 to 2019. Patients were divided into two groups: group A (357 patients) in which an endourological procedure was performed before march of 2018 (without the implementation of ISBAR tool) and Group B (100 patients) with the implementation of ISBAR tool. The inclusion criteria were patients accepted for surgical intervention by anaesthesiology Department and operated in the period of the study. The variables analysed included number of procedures, global, intraoperative and postoperative complications rate, urinary infection or sepsis, NPR (FMEA), percentage of suspended surgical patients and hospital stay. RESULTS: The postoperative complications showed no significant differences between groups, but a trend to diminishing was seen in the complication in the group B. The sepsis reduced its incidence and it was close to significant difference. The operative time was shorter in group B 119,11min (114,63-123,59) vs 115,11min (109,63-121,67) p = 0,3. The reduction in the main postoperative complication (sepsis) explained the lower hospital stay for group B. The severe adverse events detected were reduced completely. CONCLUSIONS: ISBAR tool was an effective patient safety tool improving quality care. To provide safe patient care and improving quality is indispensable an effective communication flow.

HPV knowledge and vaccine acceptability: a survey-based study among parents of adolescents (KAPPAS study).

BACKGROUND: Human papillomavirus (HPV) infection is recognized as one of the major causes of infection-related cancer worldwide. In Spain, the HPV vaccination program started in 2007 and until 2022, it targeted 12-year-old girls. METHODS: This was a cross-sectional, multicenter survey-based research carried out at 24 pediatric offices to describe HPV knowledge and vaccine acceptability in parents of children aged between 9 and 14 years-old in Spain. Parents were randomly selected from the medical records following specific quotas to ensure representativeness. The survey included five sections that aim to collect information about sociodemographic characteristics, knowledge of HPV, knowledge and acceptability of vaccines in general, HPV vaccination knowledge and HPV vaccine acceptability. Each section was constituted by a number of close questions with different answer options. Specific scores were assigned to each possible answer to these questions. Based on these scores, four composite variables were created to assess HPV knowledge, HPV vaccine knowledge, HPV vaccine acceptability and vaccines knowledge and acceptability in general. A latent class analysis was performed to identify different group of respondents according to their HPV vaccine acceptability. RESULTS: A total of 1405 valid surveys were included, with 86.19% of the respondents being mothers. The mean score of HPV knowledge was 28.92 out of 40 (maximum value) (95% CI 28.70-29.20) and the mean score of HPV vaccine acceptability was 3.37 out of 5 (maximum value). One third of parents still need more information to take a final decision about HPV vaccination in their children. Parents perceived that females were more likely to become infected than males and tended to associate HPV infection mainly with cervical cancer, showing a. a lack of information about other HPV-related diseases affecting males. CONCLUSIONS: This study results highlight the need for future actions and educational initiatives to raise awareness of HPV consequences in both genders and to contribute to achieving the elimination of HPV-related diseases beyond cervical cancer.

Modelo de gestión de la condición crónica litiásica (Kaiser Permanente) / Healthcare management protocols for chronic stone disease (Kaiser Permanente)

INTRODUCCIÓN: La enfermedad litiásica (EL) presenta una evolución crónica en un alto porcentaje de pacientes. Teniendo en cuenta el alto coste sanitario asociado al tratamiento de esta patología, deberían adaptarse y utilizarse enfoques y estrategias de cronicidad de forma similar a otras enfermedades crónicas. Uno de los modelos aplicados para la gestión de estas enfermedades con importante repercusión en el consumo de recursos sanitarios es el modelo de Kaiser Permanente. MATERIAL Y MÉTODOS: Para el desarrollo de este proyecto de gestión de la enfermedad litiásica se realizó una implementación en tres fases diferentes: Fase 1: identificación de la población objetivo del programa y diseño del modelo de asignación de riesgo. Se consideraron como factores de riesgo, la clasificación del paciente en el modelo CRG (clasificación de grupos de riesgo o carga de morbilidad) como predictor de mayor consumo de recursos, factores de riesgo anatómicos, factores de riesgo litógenos y factores hereditarios asociados a la litiasis. Fase 2: clasificación de los pacientes según riesgo y aplicación de medidas específicas. Las medidas de intervención dependerán del nivel de riesgo asignado: bajo, intermedio o alto riesgo. Fase 3: análisis de indicadores y resultados. RESULTADOS: La aplicación del modelo permitió diseñar el algoritmo y dibujar la pirámide de Kaiser: El 59% de los pacientes se asignaron al grupo de bajo riesgo y el 41% correspondían a pacientes de riesgo alto (36,5%) o muy alto (4,5%). Los resultados preliminares obtenidos a dos años de seguimiento muestran una reducción de la recidiva litiásica global en un 42,2% cuando se comparó con un grupo control (seguimiento clásico). La adherencia global del grupo intervención fue de un 96,4% y la satisfacción de los pacientes incluidos en el programa fue de 9,93/10, superiores estadísticamente al grupo control. CONCLUSIÓN: El modelo de gestión poblacional basado en la pirámide de Kaiser Permanente es factible como modelo de gestión de la condición crónica litiásica. La implantación de este modelo ha demostrado de forma preliminar su eficiencia en pacientes crónicos

INTRODUCTION: Stone disease is a chronic condition in a high percentage of patients. Due to the high healthcare costs associated with the treatment of this pathology, chronicity approaches and strategies should be adapted and used in a similar way to other chronic diseases. One of the models applied for the management of these diseases with a significant impact on the consumption of health resources is the Kaiser Permanente model. MATERIAL AND METHODS: A chronic stone disease management project was developed and carried out in three different phases: Phase 1: identification of the target population of the program and design of the risk allocation model. The risk factors considered were CRG model (classification of risk groups or burden of morbidity) as a predictor of greater consumption of resources, anatomical risk factors, lithogenic risk factors, and hereditary factors associated with lithiasis. Phase 2: classification of patients according to risk and application of specific measures. The intervention measures will depend on the level of risk assigned: low, intermediate or high risk. Phase 3: analysis of indicators and results. RESULTS: An algorithm of risk allocation was designed, and a Kaiser pyramid drawn. A total of 59% of the patients were assigned to the low-risk group and 41% corresponded to high-risk (36.5%) or very high-risk patients (4.5%). Preliminary results obtained at two years of follow-up show a reduction in global stone recurrence by 42.2% when compared with a control group (classic follow-up). The overall adherence of the intervention group was 96.4% and the satisfaction of the patients included in the program was 9.93/10. CONCLUSIONS: A management model for chronic stone disease based on the Kaiser Permanente pyramid is feasible. The implantation of this model has preliminarily demonstrated its efficiency in chronic patients

[Healthcare management protocols for chronic stone disease (Kaiser Permanente).] / Modelo de gestión de la condición crónica litiásica (Kaiser Permanente).

INTRODUCTION: Stone disease is a chronic condition in a high percentage of patients. Duento the high healthcare costs associated with the treatment of this pathology, chronicity approaches and strategies should be adapted and used in a similar way to other chronic diseases. One of the models applied for the management of these diseases with a significant impact on the consumption of health resources is the Kaiser Permanente model. MATERIAL AND METHODS: A chronic stone disease management project was developed and carried out in three different phases: Phase 1: identification of the target population of the program and design of the risk allocation model. The risk factors considered were CRG model (classification of risk groups or burden of morbidity) as a predictor of greater consumption of resources, anatomical risk factors, lithogenic risk factors, and hereditary factors associated with lithiasis. Phase 2: classification of patients according to risk and application of specific measures. The intervention measures will depend on the level of risk assigned: low, intermediate or high risk. Phase 3: analysis of indicators and results. RESULTS: An algorithm of risk allocation was designed, and a Kaiser pyramid drawn. A total of 59% of the patients were assigned to the low-risk group and 41% corresponded to high-risk (36.5%) or very high-risk patients (4.5%). Preliminary results obtained at two years of follow-up show a reduction in global stone recurrence by 42.2% when compared with a control group (classic follow-up). The overall adherence of the intervention group was 96.4% and the satisfaction of the patients included in the program was 9.93/10. CONCLUSIONS: A management model for chronic stone disease based on the Kaiser Permanente pyramidis feasible. The implantation of this model has preliminarily demonstrated its efficiency in chronic patients.

INTRODUCCIÓN: La enfermedad litiásica (EL) presenta una evolución crónica en un alto porcentaje de pacientes. Teniendo en cuenta el alto coste sanitario asociado al tratamiento de esta patología,deberían adaptarse y utilizarse enfoques y estrategias de cronicidad de forma similar a otras enfermedades crónicas. Uno de los modelos aplicados para la gestión de estas enfermedades con importante repercusión en el consumo de recursos sanitarios es el modelo de Kaiser Permanente.MATERIAL Y MÉTODOS: Para el desarrollo de este proyecto de gestión de la enfermedad litiásica se realizó una implementación en tres fases diferentes: Fase 1: identificación de la población objetivo del programa y diseño del modelo de asignación de riesgo. Se consideraron como factores de riesgo, la clasificación del paciente en el modelo CRG (clasificación de grupos de riesgo o carga de morbilidad) como predictor de mayor consumo de recursos, factores de riesgo anatómicos, factores de riesgo litógenos y factores hereditarios asociados a la litiasis. Fase 2: clasificación de los pacientes según riesgo y aplicación de medidas específicas. Las medidas de intervención dependerán del nivel de riesgo asignado: bajo, intermedio o alto riesgo. Fase 3: análisis de indicadores y resultados.RESULTADOS: La aplicación del modelo permitió diseñar el algoritmo y dibujar la pirámide de Kaiser: El 59% de los pacientes se asignaron al grupo de bajo riesgo y el 41% correspondían a pacientes de riesgo alto (36,5%) o muy alto (4,5%). Los resultados preliminares obtenidos a dos años de seguimiento muestran una reducción de la recidiva litiásica global en un 42,2% cuando se comparó con un grupo control (seguimiento clásico). La adherencia global del grupo intervención fue de un 96,4% y la satisfacción de los pacientes incluidos en el programa fue de 9,93/10, superiores estadísticamente al grupo control.CONCLUSIÓN: El modelo de gestión poblacional basado en la pirámide de Kaiser Permanente es factible como modelo de gestión de la condición crónica litiásica. La implantación de este modelo ha demostrado de forma preliminar su eficiencia en pacientes crónicos.

Characterization of tomato endo-beta-1,4-glucanase Cel1 protein in fruit during ripening and after fungal infection.

The tomato (Lycopersicon esculentum Mill.) endo-beta-1,4-glucanase (EGase) Cel1 protein was characterized in fruit using specific antibodies. Two polypeptides ranging between 51 and 52 kDa were detected in the pericarp, and polypeptides ranging between 49 and 51 kDa were detected in locules. The polypeptides recognized by Cel1 antiserum in fruit are within the size range predicted for Cel1 protein and could be derived from heterogeneous glycosylation. Cel1 protein accumulation was examined throughout fruit ripening. Cel1 protein appears in the pericarp at the stage in which many ripening-related changes start, and remains present throughout fruit ripening. In locules, Cel1 protein is already present at the onset of fruit ripening and remains constant during fruit ripening. This pattern of expression supports a possible role for this EGase in the softening of pericarp tissue and in the liquefaction of locules that takes place during ripening. The accumulation of Cel1 protein was also analyzed after fungal infection. Cel1 protein and mRNA levels are down-regulated in pericarp after Botrytis cinerea infection but are not affected in locular tissue. The same behavior was observed when fruits were infected with Penicillium expansum, another fungal pathogen. Cel1 protein and mRNA levels do not respond to wounding. These results support the idea that the tomato Cel1 EGase responds to pathogen infection and supports a relationship between EGases, plant defense responses and fruit ripening.

Identification of a copper chaperone from tomato fruits infected with Botrytis cinerea by differential display.

Differential display was used to isolate tomato genes responding to fungal infection. Here we describe the isolation and characterization of a gene that is down-regulated in tomato fruits infected with the phytopathogen Botrytis cinerea. The cDNA identified encodes a protein that shares sequence similarity to the amino terminal region of CCH, a copper chaperone from Arabidopsis thaliana, that participates in intracellular copper homeostasis by delivering Cu to the secretory pathway. The fact that this newly characterized tomato gene, referred to as LeCCH (Lycopersicon esculentum copper chaperone), be differentially expressed after fungal infection, suggests an interesting relationship between copper homeostasis and plant defense responses. LeCCH contains the conserved metal-binding domain MXCXGC but interestingly, lacks the C-terminal extension present in previously described plant members of this copper chaperone family, that seems to be involved in metallochaperone intercellular transport. This fact indicates that LeCCH is a novel plant copper chaperone that could act locally at the infection site, affecting the copper homeostasis in this particular stress situation.