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1.
Oral Maxillofac Surg ; 21(2): 201-206, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28332068

RESUMO

PURPOSE: The aim of this study was to evaluate the effects of LED and low-intensity laser on the healing of rabbit dental alveoli. METHODS: A total of 18 rabbits were divided into three groups of six; all rabbits underwent surgical extraction of the first lower right premolar. Following surgery, group 1 received LED irradiation, group 2 received laser irradiation, and group 3 were untreated controls. The initial applications of LED and laser began 48 h after tooth extraction and were given perpendicularly to the dental alveoli. The three groups were clinically evaluated for 18 days in the postoperative period. At 90 days, the rabbits were euthanized and the mandibular fragments containing the healing alveoli were collected. These fragments initially underwent computed tomography scans and bone density was measured in Hounsfield units. Following this, the fragments were processed and stained with hematoxylin and eosin for histological and histomorphometric analysis. RESULTS: The results were compatible between the clinical, histological, and histomorphometric parameters evaluated. There were no statistical differences between the LED and laser groups. However, the difference of the average bone density between laser and control group was not statistically significant. CONCLUSIONS: It was found that photobiomodulation with LASER and LED presented effects. However, only the LED demonstrated a beneficial effect on the process of bone repair in the dental alveoli of rabbits.


Assuntos
Terapia com Luz de Baixa Intensidade/métodos , Alvéolo Dental/patologia , Cicatrização/fisiologia , Animais , Densidade Óssea/fisiologia , Coelhos
2.
Cell Rep ; 14(7): 1641-1654, 2016 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-26876168

RESUMO

It is known that internal physiological state, or interoception, influences CNS function and behavior. However, the neurons and mechanisms that integrate sensory information with internal physiological state remain largely unknown. Here, we identify C. elegans body cavity neurons called URX(L/R) as central homeostatic sensors that integrate fluctuations in oxygen availability with internal metabolic state. We show that depletion of internal body fat reserves increases the tonic activity of URX neurons, which influences the magnitude of the evoked sensory response to oxygen. These responses are integrated via intracellular cGMP and Ca(2+). The extent of neuronal activity thus reflects the balance between the perception of oxygen and available fat reserves. The URX homeostatic sensor ensures that neural signals that stimulate fat loss are only deployed when there are sufficient fat reserves to do so. Our results uncover an interoceptive neuroendocrine axis that relays internal state information to the nervous system.


Assuntos
Tecido Adiposo/efeitos dos fármacos , Caenorhabditis elegans/efeitos dos fármacos , Potenciais Somatossensoriais Evocados/efeitos dos fármacos , Homeostase/efeitos dos fármacos , Oxigênio/farmacologia , Células Receptoras Sensoriais/efeitos dos fármacos , Tecido Adiposo/metabolismo , Animais , Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Proteínas de Caenorhabditis elegans/antagonistas & inibidores , Proteínas de Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/metabolismo , Cálcio/metabolismo , Dióxido de Carbono/farmacologia , GMP Cíclico/metabolismo , Potenciais Somatossensoriais Evocados/genética , Subunidades alfa de Proteínas de Ligação ao GTP/genética , Subunidades alfa de Proteínas de Ligação ao GTP/metabolismo , Regulação da Expressão Gênica , Guanilato Ciclase/genética , Guanilato Ciclase/metabolismo , Homeostase/genética , Interocepção/efeitos dos fármacos , Canais Iônicos/genética , Canais Iônicos/metabolismo , Lipase/antagonistas & inibidores , Lipase/genética , Lipase/metabolismo , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Células Receptoras Sensoriais/metabolismo , Transdução de Sinais
3.
Biochem J ; 450(2): 407-15, 2013 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-23252380

RESUMO

The halophyte Mesembryanthemum crystallinum adapts to salt stress by salt uptake and switching from C3 photosynthesis to CAM (crassulacean acid metabolism). An important role in this process is played by transport proteins in the tonoplast of the central vacuole. In the present study we examine dynamic changes in the protein composition during salt-stress adaptation in microsomes from M. crystallinum leaves. Plants challenged with 400 mM NaCl accumulate salt by day 4 of treatment and malic acid only at day 12; a switching to CAM hence follows any initial steps of salt adaptation with a delay. Using a label-free and semiquantitative approach, we identified the most dramatic changes between the proteome of control plants and plants harvested after 12 days of the treatment; the abundance of 14 proteins was significantly affected. The proteomic data revealed that the majority of the subunits of V-ATPase (vacuolar H(+)-ATPase) holoenzyme. The salt treatment somewhat decreased the abundance of all subunits in the short term (4 days). Long-term adaptation, including the switching to CAM, goes together with a strong increase in the representation of all detectable subunits. Because this increase is subunit-specific, with the highest rise occurring for subunits E and c, the data suggest that long-term adaptation to salt stress correlates with a change in V-ATPase subunit stoichiometry and highlight the structural plasticity of this holoenzyme.


Assuntos
Mesembryanthemum/enzimologia , Folhas de Planta/química , Proteínas de Plantas/química , ATPases Vacuolares Próton-Translocadoras/química , Mesembryanthemum/metabolismo , Fotossíntese , Folhas de Planta/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Proteoma/metabolismo , Proteômica , Cloreto de Sódio/metabolismo , Cloreto de Sódio/farmacologia , ATPases Vacuolares Próton-Translocadoras/genética , ATPases Vacuolares Próton-Translocadoras/metabolismo , Vacúolos/enzimologia , Vacúolos/metabolismo
4.
Odontol. clín.-cient ; 10(4): 351-3356, Out.-Dez. 2011. ilus, tab
Artigo em Inglês | LILACS | ID: lil-755703

RESUMO

Objective: The aim of this investigation was to research the basic knowledge of oral cancer in a particular population belonging to the University Campus of Minas Gerais, where all individuals under investigation were highly exposed to information in general. Methods: The sample consisted of 260 individuals distributed amongst students, professors and administrative staff. The variables "risk factor", "protective factor", "oral cancer self-examination" and "initial signals on oral cancer" were categorized, scored and graded in order to obtain a better picture of the basic knowledge on oral cancer of that population. Results: The knowledge on oral cancer seems to increase with age and women had a better knowledge than men. Considering that this population was highly exposed to information available in the campus, the level of knowledge on oral cancer found was not at all satisfactory. Conclusion: It shows the need to invest in comprehensive and long-term educational programs about oral cancer, its causes and consequences, as well as how to prevent it.


Objetivo: O objetivo desta pesquisa foi avaliar os conhecimentos básicos do câncer bucal em uma população específica do Campus Universitário de Minas Gerais, onde todos os indivíduos sob investigação são mais expostos à informação em geral. Métodos: A amostra consistiu de 260 indivíduos distribuídos entre os estudantes, professores e pessoal administrativo. As variáveis "fator de risco", "fator protetor", "autoexame para câncer bucal" e "primeiros sinais de câncer bucal" foram categorizadas, pontuadas e classificadas, a fim de se obter uma melhor imagem do conhecimento sobre câncer bucal dessa população. Resultados: O conhecimento sobre câncer bucal parece aumentar com a idade, tendo as mulheres um melhor conhecimento que os homens. Considerando que essa população é mais exposta às informações disponíveis no campus, o nível de conhecimento sobre câncer bucal encontrado não foi de todo satisfatório. Conclusão: Os resultados mostram a necessidade de investimento a longo prazo em programas educativos mais abrangentes sobre o câncer bucal, suas causas e consequências bem como a forma de se evitá-lo.

5.
Proteomics ; 11(11): 2320-8, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21598388

RESUMO

Cardiomyopathies indistinctly affect atrial and ventricular cardiac compartments with alterations of their mechanical and/or electrical activity. To understand the main mechanisms involved in these pathological alterations, a detailed knowledge of the physiology of the healthy heart is critical. In the present work, we utilize multidimensional protein identification technology to characterize the murine left ventricle (LV), right ventricle (RV), and atria (A) proteomes, identifying thousands of distinct proteins. Moreover, using multidimensional algorithm protein map tool, relative abundances of proteins among the heart chambers were investigated. In sum, we found 16 and 55 proteins were more abundant in LV compared to RV and A, respectively; 47 and 60 proteins were more abundant in RV than LV and A, respectively; and, 81 and 74 proteins were more abundant in A than LV and RV, respectively. This detailed characterization of myocardial compartment proteome represents an important advancement in the knowledge of heart physiology, and may contribute to the identification of key features underlying the onset of cardiomyopathy.


Assuntos
Átrios do Coração/química , Ventrículos do Coração/química , Proteínas/química , Proteômica/métodos , Análise de Variância , Animais , Western Blotting , Átrios do Coração/metabolismo , Ventrículos do Coração/metabolismo , Masculino , Camundongos , Especificidade de Órgãos , Fragmentos de Peptídeos , Proteínas/classificação , Proteoma/química , Tripsina
6.
J Virol Methods ; 163(2): 195-204, 2010 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19778556

RESUMO

Modified vaccinia virus Ankara (MVA) is employed as a human vaccine vector for the high expression of heterologous genes and the lack of replication in mammalian cells. This study demonstrates that cells infected by recombinant viruses can be obtained by fluorescence-activated cell sorting. Recombinant viruses are generated by a swapping event between a red fluorescent protein gene in the acceptor virus and a plasmid cassette coding for both a green fluorescent marker and a transgene. To prevent the carry-over of parental virus, due to superinfection of the cells harbouring recombinant viruses, the sorting is performed on cells infected at low m.o.i. in the presence of a reversible inhibitor of viral particle release. Terminal dilution cloning is then used to isolate both green and marker-free recombinant viruses, which can be identified by whole-plate fluoroimaging. The differential visualization of all the viral types involved allows a stepwise monitoring of all recombinations and leads to a straightforward and efficient flow cytometry-based cell sorting purification protocol. As an example of the efficacy of this sorting procedure, the construction of rMVA's coding for the rat nuclear protein HMGB1 and H5N1 influenza A virus hemagglutinin is reported. The entire recombinant MVA production process is carried out in serum-free media employing primary chicken embryo fibroblasts (CEF), which are certified for the preparation of human vaccines. This rMVA production method is faster, simpler and more reliable than any other available procedure for obtaining safe vaccine stocks for human use.


Assuntos
Genes Virais , Recombinação Genética , Vacina Antivariólica , Vaccinia virus/crescimento & desenvolvimento , Vaccinia virus/isolamento & purificação , Animais , Técnicas de Cultura de Células/métodos , Células Cultivadas , Embrião de Galinha , Galinhas , Meios de Cultura Livres de Soro , Fibroblastos/virologia , Citometria de Fluxo/métodos , Proteínas de Fluorescência Verde/genética , Proteína HMGB1/genética , Virus da Influenza A Subtipo H5N1/genética , Proteínas Luminescentes/genética , Ratos , Coloração e Rotulagem/métodos , Vaccinia virus/genética , Proteína Vermelha Fluorescente
7.
Med. oral patol. oral cir. bucal (Internet) ; 11(2): E130-E131, mar.-abr. 2006. ilus
Artigo em En | IBECS | ID: ibc-045792

RESUMO

No disponible


Oral Myiasis is a rare pathology in humans and is associated with poor oral hygiene, alcoholism, senility, supuranting lesions, severe halitosis and others conditions. The treatment is a mechanical removal of the maggots one by one but a systemic treatment with Ivermectin, a semi-synthetic macrolide antibiotic, have been used for treatment for oral myiasis. We present a case report of a 32-year-old man indigent, alcohol-dependent with an extensive necrotic area and acute swelling in upper lip and fetid odor. The patient’s manegement included topic use of gencian violet, oral therapy with ivermectin (6 mg orally), surgical exploration to remove the larvae and necrotic tissue. After the complete larvae removal the swelling and the wounds were healing normally, the patients was referred to plastic surgery to repair tissue damage. The prevention of human myiasis is by education, but unfortunately in the developing countries some people live in low social condition, predisposing the occurrence of the infestation


Assuntos
Masculino , Adulto , Humanos , Doenças Labiais/parasitologia , Doenças Labiais/terapia
8.
Med Oral Patol Oral Cir Bucal ; 11(2): E130-1, 2006 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-16505789

RESUMO

Oral Myiasis is a rare pathology in humans and is associated with poor oral hygiene, alcoholism, senility, suppurating lesions, severe halitosis and others conditions. The treatment is a mechanical removal of the maggots one by one but a systemic treatment with Ivermectin, a semi-synthetic macrolide antibiotic, have been used for treatment for oral myiasis. We present a case report of a 32-year-old man indigent, alcohol-dependent with an extensive necrotic area and acute swelling in upper lip and fetid odor. The patient's management included topic use of gentian violet, oral therapy with ivermectin (6 mg orally), surgical exploration to remove the larvae and necrotic tissue. After the complete larvae removal the swelling and the wounds were healing normally, the patients was referred to plastic surgery to repair tissue damage. The prevention of human myiasis is by education, but unfortunately in the developing countries some people live in low social condition, predisposing the occurrence of the infestation.


Assuntos
Doenças Labiais/parasitologia , Doenças Labiais/terapia , Miíase/terapia , Adulto , Humanos , Masculino
9.
Vet Res ; 36(5-6): 735-46, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-16120249

RESUMO

Alpha1-acid glycoprotein (AGP) is an immunomodulatory protein expressed by hepatocytes in response to the systemic reaction that follows tissue damage caused by inflammation, infection or trauma. This paper presents the detection of bovine AGP (boAGP) in mammary secretions (colostrum and milk) and mammary gland tissue. Bovine AGP was detected by Western blotting in all the samples analysed, and could be quantified in colostrum at 162 (+/- 63.7) microg/mL and 114.5 (+/- 67.8) microg/mL during the first 12 h and 24 h respectively. In mature milk, the boAGP concentration clearly decreased and was no longer detectable using the Radial Immunodiffusion (RID) technique. The concentration of mature milk boAGP was therefore semi-quantified using an anion-exchange chromatographic procedure that allowed the concentration of the protein to be determined. The presence of AGP in bovine milk was confirmed by the internal sequence analysis performed following purification to homogeneity of the protein from milk. The concentration of AGP in bovine milk with low SCC (< 250,000) was very similar to that from bovine milk with high SCC (> 250,000). In order to investigate the origin of AGP in bovine milk, a search for mRNA was carried out in somatic cells and mammary gland tissue: mRNA expression of the boAGP gene was detected in mammary gland tissue, but not in somatic cells. Finally, the cDNA sequence of the boAGP was determined, and is hereby presented.


Assuntos
Colostro/química , Leite/química , Orosomucoide/análise , Sequência de Aminoácidos , Animais , Sequência de Bases , Bovinos , DNA , Feminino , Expressão Gênica , Glândulas Mamárias Animais/metabolismo , Mastite Bovina/metabolismo , Dados de Sequência Molecular , RNA Mensageiro , Homologia de Sequência de Aminoácidos
10.
Vet Immunol Immunopathol ; 107(1-2): 17-26, 2005 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-15982476

RESUMO

alpha1-Acid glycoprotein (AGP) is considered one of the major acute phase proteins in cats. In humans, AGP is a heavily glycosylated protein that undergoes several modifications of its glycan moiety during acute and chronic inflammatory pathologies. In this paper we present the feline AGPs (fAGP) glycan moiety modifications in the course of two prevalent feline diseases, the FIV (feline immunodeficiency virus) dependent feline acquired viral immunodeficiency and the feline leukemia virus (FeLV) associated lymphoma. The glycan moiety of fAGP was investigated by means of the binding of its oligosaccharides residues with specific lectins. Four lectins were used: Sambucus nigra agglutinin I and Maackia amurensis agglutinin lectins were used to detect sialic acid residues, Aleuria aurantia lectin was used to detect L-fucose residues and Concanavalin A was used to evaluate the degree of branching. It was found that fAGP undergoes several post-translational modifications of its glycan pattern: in particular the degree of sialylation is increased in FeLV-positive cats diagnosed with lymphoma, while FeLV-positive that did not presented any specific clinical signs cats do not present any increase of expression of sialic acid on the surface. Furthermore, FIV induced a modification of the glycan moiety of fAGP, which however varied widely among individuals. In order to determine the number and the position of oligosaccharide chains, the cDNA sequence of fAGP was also determined. The translation of the mature fAGP coding sequence gave rise to a sequence of 183 residues, with five potential N-glycosylation sites, but also with seven potential phosphorylation sites.


Assuntos
Síndrome de Imunodeficiência Adquirida Felina/metabolismo , Vírus da Imunodeficiência Felina , Vírus da Leucemia Felina , Leucemia Felina/metabolismo , Orosomucoide/química , Sequência de Aminoácidos , Animais , Sequência de Bases , Gatos , Clonagem Molecular , DNA Complementar/genética , Síndrome de Imunodeficiência Adquirida Felina/genética , Glicosilação , Lectinas/metabolismo , Leucemia Felina/genética , Dados de Sequência Molecular , Orosomucoide/genética , Orosomucoide/metabolismo , Polissacarídeos/química , Processamento de Proteína Pós-Traducional , Homologia de Sequência de Aminoácidos
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