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1.
Parasit Vectors ; 16(1): 357, 2023 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-37817278

RESUMO

BACKGROUND: Asymptomatic infection is the most common outcome of exposure to Leishmania parasites. In the Mediterranean region, where Leishmania infantum is endemic, studies on the prevalence of asymptomatic infection have often relied on serological testing in blood donors. In Spain, regional studies have shown seroprevalence in blood donors between 1 and 8%; in Portugal, values of 0 and 2% were suggested by two localized studies, in different populations. The purpose of this study was (i) to estimate the prevalence of asymptomatic Leishmania infection in blood donors in mainland Portugal, and (ii) to study the association between the detection of antibodies to Leishmania and sociodemographic factors, and also the knowledge, perceptions and practices (KPP) of the blood donors regarding leishmaniasis. METHODS: A cross-sectional study targeted the population of people who donated blood in mainland Portugal. Participants, distributed proportionally by municipality and aged between 18 and 65 years, were selected randomly in 347 blood collection points between February and June 2022, and completed a sociodemographic and a KPP questionnaire. Detection of anti-Leishmania antibodies in serum was performed using an ELISA commercial kit. Individual KPP scores were calculated by adding grades defined for each question. RESULTS: Globally, 201/3763 samples were positive. The estimated national true seroprevalence was 4.8% (95% CI 4.1-5.5%). The proportion of positive results was significantly different between NUTS (Nomenclature of Territorial Units for Statistics) regions. Models suggested that seropositivity was significantly higher in male sex, people older than 25 years, or residing in the Centro NUTS2 region, but not in dog owners nor people with lower KPP scores. Overall, 72.3% of participants had previously heard of leishmaniasis and, in multivariate analysis, a higher Knowledge score was associated with age 25-40 years, female sex, ownership of dogs, and higher education. CONCLUSIONS: Global estimated true seroprevalence (4.8%) was similar to previous regional studies in blood donors in neighboring Spain. Higher seroprevalence values in the NUTS2 Centro region were consistent with incidence data from humans and seroprevalence studies in dogs. On the other hand, the low values in the Alentejo and the high values in the northern subregions may be the result of geographical shifts in parasite circulation due to climate change and should prompt localized and integrated, vector, canine, and human research, following a One Health approach.


Assuntos
Doenças do Cão , Leishmania infantum , Leishmaniose Visceral , Leishmaniose , Humanos , Masculino , Animais , Cães , Feminino , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Portugal/epidemiologia , Doadores de Sangue , Infecções Assintomáticas/epidemiologia , Estudos Soroepidemiológicos , Prevalência , Estudos Transversais , Doenças do Cão/parasitologia , Leishmaniose/veterinária , Anticorpos Antiprotozoários , Leishmaniose Visceral/parasitologia
2.
Eur J Immunol ; 50(12): 2025-2040, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33084029

RESUMO

SARS-CoV-2 has emerged as a human pathogen, causing clinical signs, from fever to pneumonia-COVID-19-but may remain mild or asymptomatic. To understand the continuing spread of the virus, to detect those who are and were infected, and to follow the immune response longitudinally, reliable and robust assays for SARS-CoV-2 detection and immunological monitoring are needed. We quantified IgM, IgG, and IgA antibodies recognizing the SARS-CoV-2 receptor-binding domain (RBD) or the Spike (S) protein over a period of 6 months following COVID-19 onset. We report the detailed setup to monitor the humoral immune response from over 300 COVID-19 hospital patients and healthcare workers, 2500 University staff, and 198 post-COVID-19 volunteers. Anti-SARS-CoV-2 antibody responses follow a classic pattern with a rapid increase within the first three weeks after symptoms. Although titres reduce subsequently, the ability to detect anti-SARS-CoV-2 IgG antibodies remained robust with confirmed neutralization activity for up to 6 months in a large proportion of previously virus-positive screened subjects. Our work provides detailed information for the assays used, facilitating further and longitudinal analysis of protective immunity to SARS-CoV-2. Importantly, it highlights a continued level of circulating neutralising antibodies in most people with confirmed SARS-CoV-2.


Assuntos
Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/imunologia , COVID-19/imunologia , SARS-CoV-2/imunologia , Glicoproteína da Espícula de Coronavírus/imunologia , Adulto , Idoso , Idoso de 80 Anos ou mais , COVID-19/epidemiologia , Feminino , Voluntários Saudáveis , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Soroepidemiológicos , Fatores de Tempo
3.
Transfus Apher Sci ; 59(4): 102777, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32487512

RESUMO

Infection with the protozoan parasite Toxoplasma gondii is prevalent in animals and humans worldwide. The present study aimed to evaluate the prevalence of antibodies to T. gondii and associated risk factors among blood donors in Portugal. Serum samples were tested for the presence of anti-T. gondii immunoglobulin (Ig) G by a modified agglutination test (MAT). A written standardized questionnaire was used to collect sociodemographic and behavioural data from the blood donors. Out of 520 participants (median age: 39.5 years; interquartile range: 29.0-47.0), who attended blood collection sessions promoted by the Portuguese Institute for Blood and Transplantation (IPST), 198 (38.1%) were positive for anti-T. gondii IgG (95% confidence interval [CI]: 33.9-42.4%). Multiple logistic regression analysis revealed that ages of 46-55 years (odds ratio [OR] = 6.72; 95% CI = 3.40-13.28), and of 56-65 years (OR = 4.34; 95% CI = 1.73-10.86), having a lower education level (OR = 2.55; 95% CI = 1.45-4.49), living in the North (OR = 2.14; 95% CI = 1.25-3.65) and in the Centre regions (OR = 2.54; 95% CI = 1.36-4.76) of Portugal, and drinking water from untreated sources (OR = 2.46; 95% CI = 1.12-5.39) were risk factors for seropositivity to T. gondii. This study provides the first data on the seroprevalence of T. gondii in blood donors in Portugal, as well insights to sociodemographic and behavioural risk factors as the basis for future prevention programs.


Assuntos
Doadores de Sangue/estatística & dados numéricos , Toxoplasma/metabolismo , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Portugal , Estudos Soroepidemiológicos
4.
J Healthc Eng ; 2018: 6183468, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29707185

RESUMO

This work provides an overview and appraisal of the general evolution of IS/IT in haemovigilance, from which lessons can be learned for its future strategic management. An electronic survey was conducted among the members of the International Haemovigilance Network to compile information on the mechanisms implemented to gather, process, validate, and store these data, to monitor haemovigilance activity, and to produce analytical reports. Survey responses were analysed by means of descriptive statistics, and comments/observations were considered in the final discussion. The answers received from 23 haemovigilance organizations show a direct relationship between the number of collected notifications (i.e., communication of adverse effects and events) and the technical specifications of the haemovigilance system in use. Notably, IT is used in the notification reception of 17 of these systems, out of which 8 systems are exclusively based on Web solutions. Most assessments of the evolution of IS/IT tend to focus on the scalability and flexibility of data gathering and reporting, considering the ever-changing requirements of haemovigilance. Data validation is poorly implemented, and data reporting has not reached its full potential. Web-based solutions are seen as the most intuitive and flexible for a system-user interaction.


Assuntos
Segurança do Sangue/normas , Transfusão de Sangue/normas , Tecnologia da Informação/tendências , Coleta de Dados , Previsões , Humanos , Ciência da Informação , Cooperação Internacional , Internet , Inquéritos e Questionários
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