RESUMO
Bacterial resistance to antimicrobial agents is a common problem observed in hospitals. We characterized a clinical isolate of Klebsiella pneumoniae (R-3455) which was resistant to high concentrations of broad spectrum beta-lactams, aminoglycosides, fluoroquinolones, chloramphenicol and tetracycline. Conjugation experiments showed that the multiresistance could be transferred to Escherichia coli J53-2 receptor strain. The transconjugant X-3455 was resistant to all antibacterials assayed in R-3455, except to fluoroquinolones. We found that both strains R-3455 and X-3455 produced a beta-lactamase which was sensitive to clavulanic acid. Southern hybridization and PCR analysis showed the presence of at least, a TEM type beta-lactamase gene in both strains.
Assuntos
Antibacterianos/farmacologia , Klebsiella pneumoniae/efeitos dos fármacos , Fatores R , Proteínas de Bactérias/genética , Sequência de Bases , Conjugação Genética , Resistência Microbiana a Medicamentos/genética , Genes Bacterianos , Klebsiella pneumoniae/genética , Dados de Sequência Molecular , beta-Lactamases/genética , beta-LactamasRESUMO
The importance and limitations of microscopic differential diagnosis of Entamoeba histolytica in asymptomatic carriers is reviewed. The possibility that some nonpathogenic strains of E. histolytica could be Entamoeba hartmanni is discussed. The imperative necessity to encourage research in epidemiology and diagnosis is emphasized. The need to know the prevalence in Mexico and distinguish E. histolytica from E. hartmanni is also discussed. This differential diagnosis is fundamental in the treatment of carriers and in epidemiological studies. Treatment must be directed only to acute and chronic patients and asymptomatic carries who prepare food. Appropriate drugs are still metronidazole and quinolines.
